Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABLB98_RS03730 Genome accession   NZ_CP157435
Coordinates   770558..771595 (-) Length   345 a.a.
NCBI ID   WP_178883219.1    Uniprot ID   A0A7Y8U8Q9
Organism   Acinetobacter sp. XH1639     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 765558..776595
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABLB98_RS03700 (ABLB98_03695) - 766212..766667 (+) 456 WP_004663432.1 bacteriohemerythrin -
  ABLB98_RS03705 (ABLB98_03700) - 766916..767998 (+) 1083 WP_348883160.1 hypothetical protein -
  ABLB98_RS03710 (ABLB98_03705) - 767998..768318 (+) 321 WP_087548040.1 RnfH family protein -
  ABLB98_RS03715 (ABLB98_03710) - 768372..768770 (-) 399 WP_004663429.1 outer membrane protein assembly factor BamE -
  ABLB98_RS03720 (ABLB98_03715) fur 768882..769319 (+) 438 WP_004663428.1 ferric iron uptake transcriptional regulator -
  ABLB98_RS03725 (ABLB98_03720) pilU 769415..770533 (-) 1119 WP_005158228.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABLB98_RS03730 (ABLB98_03725) pilT 770558..771595 (-) 1038 WP_178883219.1 type IV pilus twitching motility protein PilT Machinery gene
  ABLB98_RS03735 (ABLB98_03730) - 771719..772396 (+) 678 WP_256415503.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABLB98_RS03740 (ABLB98_03735) - 772509..776105 (-) 3597 WP_348883161.1 AAA family ATPase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38297.90 Da        Isoelectric Point: 6.5054

>NTDB_id=1006838 ABLB98_RS03730 WP_178883219.1 770558..771595(-) (pilT) [Acinetobacter sp. XH1639]
MDITELLAFAVKNGASDLHLSSGMPPMIRVDGEVRRINLPALEHKDVHRLVYDIMNDKQRRDYEEDLETDFSFEVPNIAR
FRVNAFNQNRGAGAVFRTIPSKVLTMEDLGLGSIFKEICDYPRGIVLVTGPTGSGKSTTLAAMIDYINENRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNQALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLVRENKVAQMYSAIQTGANYGMTTLDQSL
KALVSKGVISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1006838 ABLB98_RS03730 WP_178883219.1 770558..771595(-) (pilT) [Acinetobacter sp. XH1639]
ATGGATATTACAGAATTACTAGCATTTGCAGTTAAAAACGGTGCTTCAGACTTACATCTTTCATCAGGTATGCCACCAAT
GATTCGTGTAGATGGTGAAGTCCGTCGTATTAATTTACCAGCACTAGAACATAAAGATGTACACCGCTTAGTGTATGACA
TCATGAATGATAAACAACGTCGTGATTATGAAGAAGATCTAGAAACTGACTTTTCATTTGAAGTGCCTAATATTGCACGT
TTCCGTGTGAACGCATTTAACCAAAACCGTGGTGCTGGCGCGGTATTCCGTACCATTCCATCTAAAGTTTTAACCATGGA
AGACCTTGGTTTAGGTTCAATTTTTAAAGAAATTTGTGATTATCCACGTGGTATCGTTTTGGTTACAGGACCTACGGGTT
CTGGTAAATCAACGACGCTCGCTGCGATGATTGATTATATTAATGAAAACCGTTATGACCATATTTTAACAGTTGAAGAC
CCGATTGAATTTGTACACCAATCTAAAAAATGTTTGATTAACCAACGTGAAGTGCATCGTGATACTCATGGCTTTAACCA
AGCACTTCGTTCTGCACTTCGTGAAGACCCTGACATTATTCTTGTCGGTGAGATGCGTGACTTAGAAACCATTCGACTTG
CGTTAACTGCGGCTGAAACAGGTCACTTGGTTTTTGGTACACTGCATACGACCTCTGCAGCGAAAACAATTGACCGTGTC
ATCGACGTATTCCCTGCCGAAGAAAAAGATATGGTTCGTGCCATGCTTTCTGAATCACTACAAGCCGTTATTTCACAAAC
ATTATTGAAGAAAAATGGCGGTGGTCGTGTTGCAGCACATGAAATCATGATTGGAATTCCAGCAATTCGTAACCTTGTAC
GTGAAAATAAAGTAGCTCAGATGTATTCTGCCATCCAAACGGGTGCAAACTACGGTATGACTACACTTGACCAAAGTCTT
AAGGCCTTAGTTTCAAAAGGCGTTATTAGCCCTCAAGTTGCCCGTACTGCAGCAAAACAACCTGAGTCATTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A7Y8U8Q9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

96.232

100

0.962
  pilT Acinetobacter baumannii D1279779

95.942

100

0.959
  pilT Acinetobacter baumannii strain A118

95.942

100

0.959
  pilT Acinetobacter baylyi ADP1

86.337

99.71

0.861
  pilT Pseudomonas stutzeri DSM 10701

82.558

99.71

0.823
  pilT Pseudomonas aeruginosa PAK

82.267

99.71

0.82
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Neisseria meningitidis 8013

68.696

100

0.687
  pilT Neisseria gonorrhoeae MS11

68.116

100

0.681
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.181

96.232

0.675
  pilT Vibrio cholerae strain A1552

70.181

96.232

0.675
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.725

100

0.507
  pilU Vibrio cholerae strain A1552

41.298

98.261

0.406
  pilU Pseudomonas stutzeri DSM 10701

40.785

95.942

0.391
  pilU Acinetobacter baylyi ADP1

38.728

100

0.388

Multiple sequence alignment