Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABFV38_RS18190 Genome accession   NZ_CP157375
Coordinates   3890677..3891657 (-) Length   326 a.a.
NCBI ID   WP_348957706.1    Uniprot ID   A0AAU7FVE3
Organism   Enterobacter cloacae complex sp. Mu1197     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3885677..3896657
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFV38_RS18160 (ABFV38_18160) - 3886553..3887050 (+) 498 WP_348957702.1 SprT family zinc-dependent metalloprotease -
  ABFV38_RS18165 (ABFV38_18165) endA 3887145..3887852 (+) 708 WP_021242074.1 deoxyribonuclease I -
  ABFV38_RS18170 (ABFV38_18170) rsmE 3887904..3888635 (+) 732 WP_021242075.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  ABFV38_RS18175 (ABFV38_18175) gshB 3888655..3889602 (+) 948 WP_348957703.1 glutathione synthase -
  ABFV38_RS18180 (ABFV38_18180) - 3889690..3890250 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  ABFV38_RS18185 (ABFV38_18185) ruvX 3890250..3890666 (+) 417 WP_235402081.1 Holliday junction resolvase RuvX -
  ABFV38_RS18190 (ABFV38_18190) pilT 3890677..3891657 (-) 981 WP_348957706.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFV38_RS18195 (ABFV38_18195) - 3891675..3892376 (+) 702 WP_045909932.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFV38_RS18200 (ABFV38_18200) - 3892398..3892964 (+) 567 WP_063622418.1 YggT family protein -
  ABFV38_RS18205 (ABFV38_18205) yggU 3892961..3893257 (+) 297 WP_045909934.1 DUF167 family protein YggU -
  ABFV38_RS18210 (ABFV38_18210) - 3893261..3893854 (+) 594 WP_025912468.1 XTP/dITP diphosphatase -
  ABFV38_RS18215 (ABFV38_18215) hemW 3893847..3894989 (+) 1143 WP_348957707.1 radical SAM family heme chaperone HemW -
  ABFV38_RS18220 (ABFV38_18220) - 3895053..3895415 (+) 363 WP_348957708.1 endonuclease domain-containing protein -
  ABFV38_RS18225 (ABFV38_18225) - 3895491..3896207 (-) 717 WP_348957710.1 DUF2884 domain-containing protein -
  ABFV38_RS18230 (ABFV38_18230) - 3896264..3896590 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35413.40 Da        Isoelectric Point: 6.4968

>NTDB_id=1006390 ABFV38_RS18190 WP_348957706.1 3890677..3891657(-) (pilT) [Enterobacter cloacae complex sp. Mu1197]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPPPDVGALLKAWLNDEQQGIWWASGQVDFAATVAGGQRLRG
SAFKQMRGVSVTLRLLPRTCPQLASLGAPRAIPELLTNESGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFADALCSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=1006390 ABFV38_RS18190 WP_348957706.1 3890677..3891657(-) (pilT) [Enterobacter cloacae complex sp. Mu1197]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCCCG
CTGGCGCAGAACGGGCCGCCTTGAACCGGCACCGTTTCCTCCTCCCGATGTCGGGGCGTTGTTAAAAGCGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCCAGCGGGCAGGTGGATTTTGCCGCGACGGTGGCAGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGTGTCTCTGTGACGCTGCGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTTCACTGGG
TGCGCCGAGGGCCATCCCGGAACTGTTAACCAATGAGTCCGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGTAAAT
CCACGACGCTGGCGGCGATGGTTGATTTTCTCAACCACCACACTGACGGCCATATCCTCACGCTCGAAGATCCGGTGGAA
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATCGGTCAGCACAGCCCGTCATTTGCCGACGCGCTGTG
CAGCGCCTTACGCCAGGATCCTGACGTGATTTTGCTCGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTAACGG
CAGCAGAGACCGGGCATCTGGTGCTGGCGACCCTGCATACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGTGCGGTGCTGGCGCAGAAACTGCTTCC
CGATCTTCAGGGCGGGCGCGTCGCGCTGTATGAACTGCTGGTGAATACCGCCGCGGCGGCGAATCTCATTCGTGAAGGGA
AAACATGGCAACTTCCCGGCATCATTCAAACCGGTCAGCAGGCAGGCATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain ERS1305867

49.068

98.773

0.485
  pilT Legionella pneumophila strain Lp02

49.068

98.773

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.099

98.773

0.436
  pilU Pseudomonas stutzeri DSM 10701

39.394

100

0.399
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39
  pilU Acinetobacter baylyi ADP1

36.646

98.773

0.362

Multiple sequence alignment