Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABHI22_RS02680 Genome accession   NZ_CP156909
Coordinates   574373..575506 (-) Length   377 a.a.
NCBI ID   WP_003484093.1    Uniprot ID   A0AAI7ZI10
Organism   Xanthomonas citri pv. mangiferaeindicae strain CFBP 9184     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 573735..607855 574373..575506 within 0


Gene organization within MGE regions


Location: 573735..607855
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABHI22_RS02680 (ABHI22_02675) pilU 574373..575506 (-) 1134 WP_003484093.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABHI22_RS02685 (ABHI22_02680) - 575605..575988 (+) 384 WP_005914753.1 DUF4398 domain-containing protein -
  ABHI22_RS02690 (ABHI22_02685) - 575985..576875 (+) 891 WP_003484096.1 hypothetical protein -
  ABHI22_RS02695 (ABHI22_02690) - 577227..577445 (+) 219 WP_003484103.1 YdcH family protein -
  ABHI22_RS02700 (ABHI22_02695) - 577684..579054 (+) 1371 WP_003484107.1 pyridoxal-phosphate dependent enzyme -
  ABHI22_RS02705 (ABHI22_02700) - 579154..580341 (+) 1188 WP_003484109.1 cystathionine gamma-synthase -
  ABHI22_RS02710 (ABHI22_02705) - 580714..583830 (+) 3117 WP_003484113.1 glycosyltransferase -
  ABHI22_RS02715 (ABHI22_02710) - 583913..584707 (+) 795 WP_003484116.1 ABC transporter permease -
  ABHI22_RS02720 (ABHI22_02715) - 584704..585990 (+) 1287 WP_033482371.1 ABC transporter ATP-binding protein -
  ABHI22_RS02725 (ABHI22_02720) - 586043..588190 (+) 2148 WP_003484120.1 methyltransferase domain-containing protein -
  ABHI22_RS02730 (ABHI22_02725) - 588190..590706 (+) 2517 WP_003484122.1 glycosyltransferase family 1 protein -
  ABHI22_RS02735 (ABHI22_02730) gmd 590818..591858 (+) 1041 WP_003484125.1 GDP-mannose 4,6-dehydratase -
  ABHI22_RS02740 (ABHI22_02735) - 591836..592828 (+) 993 WP_078538893.1 NAD-dependent epimerase/dehydratase family protein -
  ABHI22_RS02745 (ABHI22_02740) - 592860..593938 (-) 1079 Protein_542 IS3 family transposase -
  ABHI22_RS02750 (ABHI22_02745) - 594167..596137 (-) 1971 WP_386265615.1 hypothetical protein -
  ABHI22_RS02755 (ABHI22_02750) - 596187..596591 (-) 405 WP_010340217.1 GtrA family protein -
  ABHI22_RS02760 (ABHI22_02755) - 596588..597886 (-) 1299 WP_003484136.1 NAD(P)/FAD-dependent oxidoreductase -
  ABHI22_RS02765 (ABHI22_02760) - 597909..598853 (-) 945 WP_033482372.1 NAD(P)-dependent oxidoreductase -
  ABHI22_RS02770 (ABHI22_02765) - 598855..599583 (-) 729 WP_003484140.1 SDR family oxidoreductase -
  ABHI22_RS02775 (ABHI22_02770) - 599586..600887 (-) 1302 WP_003484142.1 FAD-binding oxidoreductase -
  ABHI22_RS02780 (ABHI22_02775) - 600887..602275 (-) 1389 WP_003484144.1 UbiA family prenyltransferase -
  ABHI22_RS02785 (ABHI22_02780) - 602378..603364 (-) 987 WP_003484146.1 lysylphosphatidylglycerol synthase transmembrane domain-containing protein -
  ABHI22_RS02790 (ABHI22_02785) - 603380..604324 (-) 945 WP_003484149.1 electron transfer flavoprotein subunit alpha/FixB family protein -
  ABHI22_RS02795 (ABHI22_02790) - 604324..605070 (-) 747 WP_003484150.1 electron transfer flavoprotein subunit beta/FixA family protein -
  ABHI22_RS02800 (ABHI22_02795) rfbB 605306..606361 (+) 1056 WP_033482373.1 dTDP-glucose 4,6-dehydratase -
  ABHI22_RS02805 (ABHI22_02800) rfbA 606414..607301 (+) 888 WP_003484154.1 glucose-1-phosphate thymidylyltransferase RfbA -
  ABHI22_RS02810 (ABHI22_02805) rfbC 607298..607855 (+) 558 WP_033482374.1 dTDP-4-dehydrorhamnose 3,5-epimerase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41763.12 Da        Isoelectric Point: 6.1401

>NTDB_id=1003159 ABHI22_RS02680 WP_003484093.1 574373..575506(-) (pilU) [Xanthomonas citri pv. mangiferaeindicae strain CFBP 9184]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVISQRLVKGVDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYEAAAAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1003159 ABHI22_RS02680 WP_003484093.1 574373..575506(-) (pilU) [Xanthomonas citri pv. mangiferaeindicae strain CFBP 9184]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCGT
TGATGGACGAAGGCCAGGTACCGCAGTTCGAGCGCGAGCTCGAGCTCAACATGGCCATCGCCCTGCCGGACGCCGGCCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAGGTGGGCATGGTGATTCGCGCCATTCGCAGCCGGATTCCCAGCATCGA
AGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTGGGTTCGACCGGCT
CGGGCAAGTCGACCTCGCTGGCGTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATATCTGCACAAACACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACCCACGCCTTCCAGAG
CGCGCTGAAGAACGCCATGCGCGAGGCGCCGGACGTGATTCTGATCGGCGAGATCCTGGACGCCGAAACCATGGAGGCGG
CGATCGCTTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATC
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGTTGATGAACCTGGCGCTGAACCTGCGCGCGGTCATTTCCCAGCG
TTTGGTCAAGGGCGTGGACGGCCGTCGTCGACCGGCCACCGAAGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTGC
GCCGCGGCCAAGTGCACGAGGTCAAGCAGGCGATGGAAGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGGATGGTGAAGTCGGGCGAAATCGAGCAGGAAGAAGCGCTGCGCGCTGCCGACTCGCGCGACGGCCTGGCCCTGAA
GTTCCGCCTTTCCGAAGGCGGCTCCGGCGAGCACGACCCGTACGCGGACTACGAAGCGGCCGCGGCACCGAAGATCAGCC
ACGGGTTCATCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.089

95.225

0.496
  pilU Vibrio cholerae strain A1552

49.444

95.491

0.472
  pilU Acinetobacter baylyi ADP1

49.718

93.899

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.241

100

0.448
  pilT Pseudomonas aeruginosa PAK

43.195

89.655

0.387
  pilT Acinetobacter baumannii strain A118

41.716

89.655

0.374
  pilT Acinetobacter nosocomialis M2

41.716

89.655

0.374
  pilT Acinetobacter baumannii D1279779

41.716

89.655

0.374
  pilT Pseudomonas stutzeri DSM 10701

41.916

88.594

0.371
  pilT Acinetobacter baylyi ADP1

41.176

90.186

0.371
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Vibrio cholerae strain A1552

41.441

88.329

0.366
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

41.441

88.329

0.366
  pilT Neisseria gonorrhoeae MS11

39.481

92.042

0.363
  pilT Neisseria meningitidis 8013

39.481

92.042

0.363

Multiple sequence alignment