Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABEF79_RS12140 Genome accession   NZ_CP156810
Coordinates   2472277..2473398 (+) Length   373 a.a.
NCBI ID   WP_034585991.1    Uniprot ID   A0A0A3W5X9
Organism   Acinetobacter sp. ANC 7454     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2467277..2478398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF79_RS12125 (ABEF79_12125) - 2469255..2470361 (+) 1107 WP_347460765.1 acyltransferase -
  ABEF79_RS12130 (ABEF79_12130) - 2470397..2471083 (-) 687 WP_347453616.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABEF79_RS12135 (ABEF79_12135) pilT 2471207..2472244 (+) 1038 WP_034585994.1 type IV pilus twitching motility protein PilT Machinery gene
  ABEF79_RS12140 (ABEF79_12140) pilU 2472277..2473398 (+) 1122 WP_034585991.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABEF79_RS12145 (ABEF79_12145) fur 2473466..2473903 (-) 438 WP_034585989.1 ferric iron uptake transcriptional regulator -
  ABEF79_RS12150 (ABEF79_12150) bamE 2474016..2474414 (+) 399 WP_034585986.1 outer membrane protein assembly factor BamE -
  ABEF79_RS12155 (ABEF79_12155) - 2474524..2474850 (-) 327 WP_347460766.1 RnfH family protein -
  ABEF79_RS12160 (ABEF79_12160) - 2474850..2475929 (-) 1080 WP_347453613.1 hypothetical protein -
  ABEF79_RS12165 (ABEF79_12165) - 2476070..2476525 (-) 456 WP_347453612.1 bacteriohemerythrin -
  ABEF79_RS12170 (ABEF79_12170) - 2476778..2477641 (-) 864 WP_004679438.1 IS982 family transposase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 41557.71 Da        Isoelectric Point: 6.9356

>NTDB_id=1003010 ABEF79_RS12140 WP_034585991.1 2472277..2473398(+) (pilU) [Acinetobacter sp. ANC 7454]
MDFNGLLDYMVAQKASDLFITADVEPSIKVNGTIVPIGNVKLPGSAVGQILHSIMSEKQRKEFAETRECNFAITNASKSA
RFRVSAFQQRDQPGMVLRRIETVIPTMDDLKLPPILKELAMTKRGIIIFVGATGTGKSTSLASMIGYRNENSKGHIITIE
DPIEFVHQHKGCIITQREVGIDTDSFEVALKNTLRQAPDVILIGEIRSRETMDYAIAFAETGHLVFATLHANNANQAIDR
IIHFFEADRHSQLFMDLSLNLRAMVAQQLIPTIDGTGRRAAIEILINSPLVSDLIRKGDVHEIKDLMKRSRELGMQTFDQ
ALYDLYKAKQISYKDALKHADSPNDLRLQIKLSEEGGAHLMNSHSNITFDGQS

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=1003010 ABEF79_RS12140 WP_034585991.1 2472277..2473398(+) (pilU) [Acinetobacter sp. ANC 7454]
ATGGATTTTAACGGTTTACTTGATTACATGGTGGCACAAAAGGCATCGGACTTGTTCATTACTGCCGATGTTGAGCCATC
CATTAAAGTAAATGGCACAATTGTGCCGATTGGCAACGTGAAACTGCCGGGTTCTGCTGTAGGGCAAATTCTGCATTCGA
TCATGTCGGAAAAGCAGCGCAAAGAATTTGCTGAAACCCGTGAATGTAACTTTGCCATTACCAATGCCAGCAAATCGGCA
CGTTTCCGTGTCAGTGCTTTCCAGCAGCGCGACCAGCCAGGTATGGTGTTGCGTCGGATTGAAACCGTGATTCCGACCAT
GGATGATCTGAAGTTACCGCCGATTCTAAAAGAACTGGCAATGACCAAGCGCGGGATTATCATTTTCGTAGGGGCAACCG
GTACTGGTAAATCAACCTCATTGGCTTCGATGATTGGCTACCGGAATGAAAATTCCAAAGGCCACATCATTACCATTGAA
GACCCGATCGAATTCGTGCACCAGCATAAGGGCTGTATCATTACCCAGCGTGAGGTCGGCATCGATACCGATTCTTTCGA
AGTTGCGCTGAAAAACACCCTGCGTCAGGCACCCGATGTGATCCTGATCGGTGAGATCCGTTCCCGCGAGACCATGGACT
ATGCCATTGCCTTTGCAGAAACCGGTCACCTGGTGTTTGCTACCCTGCACGCCAATAACGCCAACCAGGCGATTGACCGG
ATCATTCACTTCTTTGAAGCGGATCGTCATAGCCAGCTGTTTATGGACTTGTCCCTGAACTTACGTGCCATGGTGGCCCA
ACAGCTGATTCCTACCATTGATGGTACCGGCCGACGTGCAGCAATTGAAATTCTGATCAACTCGCCACTGGTTTCAGATC
TGATCCGTAAAGGTGATGTACATGAGATCAAGGATTTGATGAAGCGTTCACGTGAACTCGGTATGCAGACTTTCGACCAG
GCATTGTATGACCTGTATAAGGCCAAACAGATCAGCTATAAAGATGCACTGAAACATGCCGACTCACCAAATGACTTGCG
TCTGCAAATCAAGCTTTCTGAGGAAGGCGGTGCCCATCTGATGAATTCACATAGCAACATCACATTTGATGGCCAGTCTT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0A3W5X9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Acinetobacter baylyi ADP1

73.28

100

0.743
  pilU Pseudomonas stutzeri DSM 10701

68.733

99.464

0.684
  pilU Vibrio cholerae strain A1552

55.676

99.196

0.552
  pilT Legionella pneumophila strain Lp02

44.643

90.08

0.402
  pilT Legionella pneumophila strain ERS1305867

44.643

90.08

0.402
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.064

92.761

0.399
  pilT Acinetobacter baumannii D1279779

42.566

91.957

0.391
  pilT Acinetobacter nosocomialis M2

42.566

91.957

0.391
  pilT Acinetobacter baumannii strain A118

42.566

91.957

0.391
  pilT Pseudomonas aeruginosa PAK

41.04

92.761

0.381
  pilT Neisseria gonorrhoeae MS11

40.936

91.689

0.375
  pilT Neisseria meningitidis 8013

40.936

91.689

0.375
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

39.886

94.102

0.375
  pilT Vibrio cholerae strain A1552

39.886

94.102

0.375
  pilT Acinetobacter baylyi ADP1

40.816

91.957

0.375
  pilT Pseudomonas stutzeri DSM 10701

40.462

92.761

0.375

Multiple sequence alignment