Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABEF83_RS11565 Genome accession   NZ_CP156768
Coordinates   2385526..2386563 (+) Length   345 a.a.
NCBI ID   WP_034585994.1    Uniprot ID   -
Organism   Acinetobacter thermotolerans strain ANC 7974     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2380526..2391563
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABEF83_RS11555 (ABEF83_11555) - 2383574..2384680 (+) 1107 WP_347453617.1 acyltransferase -
  ABEF83_RS11560 (ABEF83_11560) - 2384716..2385402 (-) 687 WP_347453616.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABEF83_RS11565 (ABEF83_11565) pilT 2385526..2386563 (+) 1038 WP_034585994.1 type IV pilus twitching motility protein PilT Machinery gene
  ABEF83_RS11570 (ABEF83_11570) pilU 2386596..2387717 (+) 1122 WP_034585991.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABEF83_RS11575 (ABEF83_11575) fur 2387784..2388221 (-) 438 WP_347453615.1 ferric iron uptake transcriptional regulator -
  ABEF83_RS11580 (ABEF83_11580) - 2388334..2388732 (+) 399 WP_034585986.1 outer membrane protein assembly factor BamE -
  ABEF83_RS11585 (ABEF83_11585) - 2388842..2389168 (-) 327 WP_347453614.1 RnfH family protein -
  ABEF83_RS11590 (ABEF83_11590) - 2389168..2390247 (-) 1080 WP_347453613.1 hypothetical protein -
  ABEF83_RS11595 (ABEF83_11595) - 2390388..2390843 (-) 456 WP_347453612.1 bacteriohemerythrin -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38238.85 Da        Isoelectric Point: 6.5056

>NTDB_id=1002556 ABEF83_RS11565 WP_034585994.1 2385526..2386563(+) (pilT) [Acinetobacter thermotolerans strain ANC 7974]
MDITELLAFSAKNGASDLHLSAGMPPLIRVDGEVRRINLPALEHKEVHKLVYDIMNDKQRRDYEENLETDFSFEVPGVAR
FRVNAFNQNRGAGAVFRTIPSKVLTIEDLGMGQIFKDICEYPRGLVLVTGPTGSGKSTTLAAMLDYINDTRYDHILTVED
PIEFVHQSKKCLINQREVHRDTHGFNEALRSALREDPDIILVGEMRDLETIRLALTAAETGHLVFGTLHTTSAAKTIDRV
IDVFPAEEKDMVRAMLSESLQAVISQTLLKKNGGGRVAAHEIMIGIPAIRNLIRENKVAQMYSAIQTGANYGMTTLDQSL
KTLVSKGIISPQVARTAAKQPESFL

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1002556 ABEF83_RS11565 WP_034585994.1 2385526..2386563(+) (pilT) [Acinetobacter thermotolerans strain ANC 7974]
ATGGACATTACAGAATTACTGGCGTTTTCTGCTAAGAATGGCGCGTCGGATTTACACTTGTCTGCGGGGATGCCACCACT
GATTCGTGTGGATGGTGAAGTACGTCGTATCAACCTGCCTGCTTTAGAACACAAAGAGGTTCACAAGCTGGTTTATGACA
TCATGAACGATAAACAGCGCCGTGACTATGAAGAAAACCTAGAAACTGACTTTTCCTTTGAAGTTCCTGGGGTTGCGCGC
TTCCGTGTGAACGCATTTAACCAGAACCGCGGTGCAGGCGCGGTATTCCGTACCATTCCATCAAAAGTTTTAACAATTGA
AGATCTTGGTATGGGCCAGATTTTTAAAGATATCTGCGAATATCCTCGTGGACTGGTACTCGTAACAGGTCCTACCGGTT
CTGGTAAATCAACCACATTGGCTGCGATGCTGGATTACATTAACGATACACGTTATGACCACATCCTGACCGTCGAAGAC
CCGATCGAATTTGTGCACCAATCCAAGAAGTGTCTGATCAACCAGCGTGAAGTGCACCGTGATACGCATGGCTTCAACGA
AGCGCTGCGTTCGGCACTGCGTGAAGACCCCGATATTATCCTGGTGGGTGAGATGCGTGACCTAGAAACCATCCGTCTGG
CACTGACTGCAGCGGAAACAGGTCACCTTGTATTTGGTACCCTGCACACCACCTCAGCAGCAAAAACCATCGACCGTGTG
ATCGACGTCTTCCCTGCTGAAGAAAAAGACATGGTCCGTGCCATGCTGTCAGAATCACTGCAAGCCGTAATTTCGCAAAC
CCTGCTCAAGAAAAACGGCGGTGGCCGTGTGGCAGCACATGAAATCATGATCGGTATTCCTGCGATCCGTAACCTGATCC
GTGAAAACAAGGTTGCACAGATGTACTCTGCAATCCAGACCGGTGCCAACTACGGCATGACCACGCTGGATCAAAGCCTG
AAAACGCTGGTGTCCAAAGGTATTATCAGTCCTCAAGTGGCACGTACTGCAGCGAAACAGCCTGAAAGCTTCCTATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

95.072

100

0.951
  pilT Acinetobacter baumannii D1279779

94.783

100

0.948
  pilT Acinetobacter baumannii strain A118

94.783

100

0.948
  pilT Acinetobacter baylyi ADP1

87.5

99.71

0.872
  pilT Pseudomonas aeruginosa PAK

84.593

99.71

0.843
  pilT Pseudomonas stutzeri DSM 10701

84.302

99.71

0.841
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Neisseria meningitidis 8013

68.986

100

0.69
  pilT Neisseria gonorrhoeae MS11

68.406

100

0.684
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.88

96.232

0.672
  pilT Vibrio cholerae strain A1552

69.88

96.232

0.672
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

52.174

100

0.522
  pilU Vibrio cholerae strain A1552

41.593

98.261

0.409
  pilU Pseudomonas stutzeri DSM 10701

41.088

95.942

0.394
  pilU Acinetobacter baylyi ADP1

38.439

100

0.386

Multiple sequence alignment