Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABFY19_RS05885 Genome accession   NZ_CP156677
Coordinates   1273337..1274395 (+) Length   352 a.a.
NCBI ID   WP_418792321.1    Uniprot ID   -
Organism   Phosphitispora sp. TUW77     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1268337..1279395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFY19_RS05865 (ABFY19_05830) - 1269338..1269823 (+) 486 WP_418792544.1 YqeG family HAD IIIA-type phosphatase -
  ABFY19_RS05870 (ABFY19_05835) - 1270022..1270891 (+) 870 WP_418792318.1 shikimate dehydrogenase -
  ABFY19_RS05875 (ABFY19_05840) - 1270895..1271386 (+) 492 WP_418792319.1 MerR family transcriptional regulator -
  ABFY19_RS05880 (ABFY19_05845) gspE 1271568..1273274 (+) 1707 WP_418792320.1 type II secretion system ATPase GspE -
  ABFY19_RS05885 (ABFY19_05850) pilT 1273337..1274395 (+) 1059 WP_418792321.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFY19_RS05890 (ABFY19_05855) - 1274410..1275627 (+) 1218 WP_418792322.1 type II secretion system F family protein -
  ABFY19_RS05895 (ABFY19_05860) - 1275706..1276056 (+) 351 WP_418792323.1 competence type IV pilus major pilin ComGC -
  ABFY19_RS05900 (ABFY19_05865) - 1276164..1276910 (+) 747 WP_418792324.1 prepilin peptidase -
  ABFY19_RS05905 (ABFY19_05870) - 1276958..1277359 (+) 402 WP_418792325.1 type IV pilus modification PilV family protein -
  ABFY19_RS05910 (ABFY19_05875) - 1277368..1277883 (+) 516 WP_418792326.1 PulJ/GspJ family protein -
  ABFY19_RS05915 (ABFY19_05880) - 1277900..1279147 (+) 1248 WP_418792327.1 DUF7305 domain-containing protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 38846.66 Da        Isoelectric Point: 6.4214

>NTDB_id=1002042 ABFY19_RS05885 WP_418792321.1 1273337..1274395(+) (pilT) [Phosphitispora sp. TUW77]
MRIDDLLKNAVEMGASDLHITTNASPVLRINGSTNFISGEKISREEVSELAEQIMGEKQKEVFEQRGEVDFSYAIFGFGR
FRVNIFKQRGSVAISIRIVPPTILTPEELGLPEVVKSLARKPNGLVLVTGPTGSGKSTTLAAMIDLINKEEKGHIITLED
PIEFLHTHRNCIVNQREVSIDTETFASGLRSALRQDPDVILVGEMRDLETISTAITAAETGHLVLATLHTSNAVQTVDRI
IDVFPPHQQNQVRVQLALILEGIIAQTLLPRADGKGRAAAVEVLVATTAVRNLIREGKTHQLPTIIQSSLKLGMQTMDNS
LQDLYMKGIISYDEARKHTLDQENFARLVKKF

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=1002042 ABFY19_RS05885 WP_418792321.1 1273337..1274395(+) (pilT) [Phosphitispora sp. TUW77]
ATGCGTATTGATGACTTGTTGAAAAACGCAGTTGAGATGGGGGCTTCAGACCTTCATATTACTACCAATGCTTCGCCTGT
ACTGAGAATAAACGGAAGCACTAATTTTATATCAGGTGAAAAAATAAGCAGGGAAGAAGTATCTGAGCTTGCCGAACAAA
TTATGGGAGAAAAACAAAAAGAAGTTTTTGAACAGCGGGGGGAAGTTGACTTTTCGTATGCCATTTTTGGTTTTGGCCGT
TTCCGGGTCAATATTTTTAAACAACGGGGTTCTGTTGCCATCTCTATTAGGATTGTACCTCCAACAATACTTACTCCTGA
GGAACTCGGACTGCCTGAAGTGGTCAAGTCATTAGCCAGGAAGCCCAATGGACTGGTGCTCGTAACCGGGCCTACCGGAA
GCGGTAAATCTACGACCTTGGCAGCTATGATTGACCTTATTAATAAAGAGGAAAAGGGTCATATTATTACACTTGAGGAC
CCGATTGAGTTTTTACATACCCACCGGAACTGTATTGTAAATCAGAGAGAAGTCAGTATTGATACGGAAACATTTGCATC
AGGCCTGCGGTCTGCGCTGAGGCAAGACCCTGATGTGATACTGGTAGGTGAGATGAGGGACCTGGAAACTATATCAACCG
CGATTACGGCTGCGGAAACAGGCCACCTTGTTTTGGCAACCCTGCACACCAGCAATGCGGTGCAGACAGTTGACCGGATT
ATAGATGTATTCCCACCGCACCAGCAAAACCAGGTTAGGGTCCAGTTGGCATTAATACTGGAAGGTATAATTGCCCAGAC
CTTGCTGCCAAGGGCTGATGGCAAAGGACGGGCAGCGGCGGTTGAGGTGCTTGTGGCCACCACTGCTGTCCGCAATCTGA
TCAGGGAAGGCAAGACACACCAGTTGCCTACAATTATCCAATCAAGTCTGAAGCTGGGTATGCAGACAATGGATAATTCA
CTCCAGGATTTGTATATGAAAGGGATTATCAGTTATGATGAAGCCCGTAAACACACACTGGATCAAGAAAACTTTGCCAG
ATTGGTGAAGAAATTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(7-269)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.585

98.58

0.509
  pilT Pseudomonas aeruginosa PAK

50.435

98.011

0.494
  pilT Pseudomonas stutzeri DSM 10701

50.145

98.011

0.491
  pilT Vibrio cholerae strain A1552

49.568

98.58

0.489
  pilT Neisseria meningitidis 8013

49.568

98.58

0.489
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.568

98.58

0.489
  pilT Acinetobacter baylyi ADP1

49.855

98.011

0.489
  pilT Neisseria gonorrhoeae MS11

49.28

98.58

0.486
  pilT Legionella pneumophila strain Lp02

48.986

98.011

0.48
  pilT Legionella pneumophila strain ERS1305867

48.986

98.011

0.48
  pilT Acinetobacter nosocomialis M2

48.696

98.011

0.477
  pilT Acinetobacter baumannii D1279779

48.696

98.011

0.477
  pilT Acinetobacter baumannii strain A118

48.696

98.011

0.477
  pilU Pseudomonas stutzeri DSM 10701

47.727

100

0.477
  pilU Acinetobacter baylyi ADP1

44.16

99.716

0.44
  pilU Vibrio cholerae strain A1552

43.875

99.716

0.438

Multiple sequence alignment