Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABDB91_RS10655 Genome accession   NZ_CP156660
Coordinates   2215910..2216980 (-) Length   356 a.a.
NCBI ID   WP_347487663.1    Uniprot ID   -
Organism   Desulfoscipio sp. XC116     
Function   mediate the depolymerization of the type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2210910..2221980
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABDB91_RS10625 (ABDB91_10625) pilO 2211708..2212241 (-) 534 WP_347487651.1 type 4a pilus biogenesis protein PilO -
  ABDB91_RS10630 (ABDB91_10630) - 2212279..2212992 (-) 714 WP_347487653.1 hypothetical protein -
  ABDB91_RS10635 (ABDB91_10635) pilM 2212996..2214273 (-) 1278 WP_347487656.1 type IV pilus assembly protein PilM -
  ABDB91_RS10640 (ABDB91_10640) - 2214336..2214473 (-) 138 WP_347487658.1 sigma factor-like helix-turn-helix DNA-binding protein -
  ABDB91_RS10645 (ABDB91_10645) - 2214460..2214663 (-) 204 WP_347487659.1 hypothetical protein -
  ABDB91_RS10650 (ABDB91_10650) - 2214668..2215876 (-) 1209 WP_347487662.1 type II secretion system F family protein -
  ABDB91_RS10655 (ABDB91_10655) pilT 2215910..2216980 (-) 1071 WP_347487663.1 type IV pilus twitching motility protein PilT Machinery gene
  ABDB91_RS10660 (ABDB91_10660) - 2217144..2218814 (-) 1671 WP_347487665.1 ATPase, T2SS/T4P/T4SS family -
  ABDB91_RS10665 (ABDB91_10665) aroB 2219196..2220287 (-) 1092 WP_347487667.1 3-dehydroquinate synthase -
  ABDB91_RS10670 (ABDB91_10670) - 2220284..2220805 (-) 522 WP_347487668.1 shikimate kinase -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 39512.65 Da        Isoelectric Point: 6.8387

>NTDB_id=1001912 ABDB91_RS10655 WP_347487663.1 2215910..2216980(-) (pilT) [Desulfoscipio sp. XC116]
MDLNDILLQTVAMNGSDLHLAAGIPPVVRINGELLRLSMPRLMPQDIEALVFPLLEPEQRNQLEQNRELDFSYSVPGLSR
FRGNIMWQRGSLGVNFRVVAMQIPLLDELGLPGVVRDLIMLPRGLVLVTGPTGSGKSTTLAALINNINEQRGLNIVTVED
PIEYLHSHKQSIVKQREVGNDTHSYAAALRHVLRHDPDVILIGEMRDTDSIAIALTAAETGHLVFSTLHTQTAALAVHRI
VDVFPEGMRNQIRQQLADSLQGVLAQQLIPRQNGRGRVAAVEILLTNPAVRNLIREAKEHQLYTVMQTGRGAGMQTMDQS
LAELCLAGIISRENALERSVDKVELERALRKRNYML

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=1001912 ABDB91_RS10655 WP_347487663.1 2215910..2216980(-) (pilT) [Desulfoscipio sp. XC116]
ATGGATTTAAATGATATATTGTTGCAAACAGTAGCCATGAACGGTTCAGACTTGCATTTGGCGGCCGGCATACCTCCGGT
AGTGCGGATCAACGGGGAATTGCTCAGGCTTTCCATGCCCCGTCTGATGCCGCAAGATATTGAAGCGTTAGTCTTTCCCT
TGCTTGAGCCCGAGCAAAGGAACCAACTGGAACAAAACCGGGAGCTGGATTTTTCTTATTCGGTGCCCGGCCTCAGTCGC
TTTAGAGGCAACATCATGTGGCAGCGCGGTTCTCTGGGGGTTAATTTTCGGGTGGTAGCCATGCAAATACCTCTTTTGGA
TGAACTGGGCCTGCCGGGGGTAGTGCGGGATTTGATAATGCTGCCTCGCGGATTGGTGCTGGTTACCGGGCCTACCGGCA
GCGGCAAATCCACCACTCTGGCGGCGCTGATCAATAATATTAATGAACAGCGCGGTTTAAACATTGTTACGGTGGAGGAT
CCTATCGAATATTTGCACAGTCATAAGCAATCAATAGTTAAGCAGCGCGAGGTGGGCAATGACACTCATTCTTACGCCGC
CGCTTTAAGACATGTGCTGCGGCATGATCCCGATGTTATTCTAATCGGTGAAATGCGGGACACCGATAGTATTGCTATCG
CGCTGACCGCTGCGGAAACCGGGCACCTGGTATTCTCTACCCTGCATACCCAGACGGCGGCTTTGGCCGTGCACCGCATA
GTGGATGTATTTCCCGAAGGCATGCGTAATCAGATCAGGCAGCAGCTGGCCGATTCTTTACAGGGAGTGCTTGCTCAGCA
ACTAATACCGCGCCAAAATGGCCGGGGGAGGGTGGCTGCGGTTGAAATATTGCTTACGAACCCGGCGGTGCGGAATTTAA
TTCGCGAGGCTAAGGAACATCAGCTATACACTGTCATGCAAACCGGGCGCGGTGCGGGTATGCAAACTATGGACCAGTCC
CTGGCCGAATTATGCCTGGCGGGTATCATATCAAGGGAAAATGCTCTGGAACGGTCCGTGGATAAAGTAGAGTTGGAAAG
AGCGCTGCGCAAGCGGAATTATATGTTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(113-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.144

97.472

0.489
  pilT Acinetobacter baylyi ADP1

50

96.067

0.48
  pilT Neisseria meningitidis 8013

49.706

95.506

0.475
  pilT Neisseria gonorrhoeae MS11

49.412

95.506

0.472
  pilT Legionella pneumophila strain Lp02

48.397

96.348

0.466
  pilT Legionella pneumophila strain ERS1305867

48.397

96.348

0.466
  pilT Acinetobacter nosocomialis M2

49.552

94.101

0.466
  pilT Acinetobacter baumannii strain A118

49.552

94.101

0.466
  pilT Acinetobacter baumannii D1279779

49.552

94.101

0.466
  pilT Pseudomonas aeruginosa PAK

48.673

95.225

0.463
  pilT Vibrio cholerae strain A1552

48.788

92.697

0.452
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.788

92.697

0.452
  pilT Pseudomonas stutzeri DSM 10701

47.198

95.225

0.449
  pilU Vibrio cholerae strain A1552

42.56

94.382

0.402
  pilU Pseudomonas stutzeri DSM 10701

40.476

94.382

0.382
  pilU Acinetobacter baylyi ADP1

38.824

95.506

0.371

Multiple sequence alignment