Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABDB91_RS10540 Genome accession   NZ_CP156660
Coordinates   2195087..2196151 (-) Length   354 a.a.
NCBI ID   WP_347487617.1    Uniprot ID   -
Organism   Desulfoscipio sp. XC116     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2190087..2201151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABDB91_RS10520 (ABDB91_10520) - 2191165..2191380 (-) 216 WP_347487610.1 hypothetical protein -
  ABDB91_RS10525 (ABDB91_10525) - 2191662..2191925 (+) 264 WP_347487612.1 DUF3243 domain-containing protein -
  ABDB91_RS10530 (ABDB91_10530) - 2192565..2193320 (-) 756 WP_347487614.1 prepilin peptidase -
  ABDB91_RS10535 (ABDB91_10535) - 2193457..2194854 (-) 1398 WP_347487616.1 TldD/PmbA family protein -
  ABDB91_RS10540 (ABDB91_10540) pilT 2195087..2196151 (-) 1065 WP_347487617.1 type IV pilus twitching motility protein PilT Machinery gene
  ABDB91_RS10545 (ABDB91_10545) - 2196437..2197693 (+) 1257 WP_347487619.1 hypothetical protein -
  ABDB91_RS10550 (ABDB91_10550) - 2197946..2200021 (+) 2076 WP_347487621.1 hypothetical protein -
  ABDB91_RS10555 (ABDB91_10555) - 2200006..2200449 (+) 444 WP_347487622.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  ABDB91_RS10560 (ABDB91_10560) - 2200451..2201050 (+) 600 WP_347487624.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 39270.38 Da        Isoelectric Point: 7.7792

>NTDB_id=1001911 ABDB91_RS10540 WP_347487617.1 2195087..2196151(-) (pilT) [Desulfoscipio sp. XC116]
MHINNLLKQALDRGASDLHIIAGVAPLLRINGELTSLKFPPLTADNTRELARQILNKEKLNVLEQLGEVDLSAEYQGLGY
FRVNVYYQRGTVGIACRVLSNRIPTIEELKLPDSIKALARYTRGLVIITGPTGSGKSTTLAAMINLINEETLCHVITLED
PIEYRHDNKKSIINQREIGRDSKSFPGALRAALRQDPDVILVGEMRDLETISIAITAAETGHLVLTTLHTMDAPQTVERI
IDVFPPNQQQQIRVQLANTLVGVVSQRLLRNSNGQGRIPAVEVLVCTPAVRNLIREKKIHQIYSYMQTGGKFGMQTMDSH
LLELHGGGLVNTKDVIEHAVDLEAMLRCLSCQQR

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=1001911 ABDB91_RS10540 WP_347487617.1 2195087..2196151(-) (pilT) [Desulfoscipio sp. XC116]
ATGCACATCAACAATTTACTTAAACAAGCGCTGGATCGGGGTGCCTCTGACTTGCATATTATAGCGGGAGTTGCACCTTT
ATTAAGAATAAATGGTGAACTGACTTCATTAAAATTTCCACCGTTAACAGCTGATAATACCCGGGAATTAGCCAGGCAAA
TACTTAACAAGGAAAAACTAAACGTGCTGGAACAGCTTGGTGAGGTGGATTTGTCCGCTGAATATCAGGGTTTGGGGTAT
TTTCGGGTAAATGTTTATTACCAACGAGGCACGGTGGGTATTGCCTGTCGGGTGCTCAGCAACCGAATTCCCACTATTGA
GGAACTAAAATTACCCGATTCCATAAAGGCCCTGGCCCGGTATACACGGGGATTGGTCATTATTACAGGGCCGACGGGCA
GCGGTAAATCTACCACACTGGCCGCAATGATTAACCTTATTAATGAAGAGACGCTGTGCCACGTGATCACGCTGGAAGAT
CCGATTGAATACCGGCACGATAACAAGAAAAGTATTATTAACCAAAGGGAAATCGGCAGGGACAGCAAATCTTTCCCCGG
TGCGCTGCGGGCGGCGCTGCGGCAGGATCCCGATGTTATACTGGTGGGCGAGATGCGGGATCTGGAAACCATATCTATTG
CTATAACCGCCGCTGAAACGGGCCATCTGGTCTTGACTACTCTGCATACTATGGACGCTCCCCAAACGGTGGAAAGAATC
ATTGACGTATTTCCCCCCAATCAGCAGCAGCAGATAAGAGTCCAGCTGGCTAATACATTGGTGGGGGTGGTCTCCCAGCG
CCTTTTGCGGAATAGTAACGGGCAGGGACGGATACCTGCCGTGGAAGTGCTGGTATGTACCCCGGCGGTGCGTAATTTGA
TTCGGGAGAAAAAGATTCATCAAATTTACTCTTATATGCAAACCGGCGGCAAGTTCGGCATGCAGACCATGGACAGCCAT
CTATTAGAACTGCACGGCGGAGGTCTGGTCAATACCAAGGACGTCATAGAGCATGCTGTGGACTTGGAAGCTATGTTGCG
CTGCTTAAGTTGTCAACAGAGGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(5-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

51.009

98.023

0.5
  pilT Vibrio cholerae strain A1552

48.657

94.633

0.46
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.657

94.633

0.46
  pilT Neisseria meningitidis 8013

47.11

97.74

0.46
  pilT Neisseria gonorrhoeae MS11

46.821

97.74

0.458
  pilT Legionella pneumophila strain Lp02

47.214

96.328

0.455
  pilT Legionella pneumophila strain ERS1305867

47.214

96.328

0.455
  pilT Acinetobacter nosocomialis M2

46.243

97.74

0.452
  pilT Acinetobacter baumannii D1279779

46.243

97.74

0.452
  pilT Acinetobacter baumannii strain A118

46.243

97.74

0.452
  pilT Pseudomonas aeruginosa PAK

46.608

95.763

0.446
  pilT Acinetobacter baylyi ADP1

47.734

93.503

0.446
  pilT Pseudomonas stutzeri DSM 10701

45.723

95.763

0.438
  pilU Pseudomonas stutzeri DSM 10701

40.857

98.87

0.404
  pilU Vibrio cholerae strain A1552

39.886

99.153

0.395
  pilU Acinetobacter baylyi ADP1

41.176

96.045

0.395

Multiple sequence alignment