Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ABFU76_RS05160 Genome accession   NZ_CP155979
Coordinates   1231609..1232016 (+) Length   135 a.a.
NCBI ID   WP_228442818.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain CFBP 6580     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1226609..1237016
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU76_RS05140 (ABFU76_05130) - 1228040..1228295 (-) 256 Protein_997 SymE family type I addiction module toxin -
  ABFU76_RS05145 (ABFU76_05135) coaE 1228489..1229112 (-) 624 WP_225042504.1 dephospho-CoA kinase -
  ABFU76_RS05150 (ABFU76_05140) - 1229126..1229989 (-) 864 WP_040940797.1 A24 family peptidase -
  ABFU76_RS05155 (ABFU76_05145) pilC 1229996..1231258 (-) 1263 WP_228442817.1 type II secretion system F family protein Machinery gene
  ABFU76_RS05160 (ABFU76_05150) pilA/pilAI 1231609..1232016 (+) 408 WP_228442818.1 pilin Machinery gene
  ABFU76_RS05165 (ABFU76_05155) - 1232149..1232535 (+) 387 WP_373046337.1 pilin -
  ABFU76_RS05170 (ABFU76_05160) - 1232594..1234414 (+) 1821 WP_323539564.1 hypothetical protein -
  ABFU76_RS05175 (ABFU76_05165) - 1234523..1235281 (+) 759 WP_228442820.1 class I SAM-dependent methyltransferase -
  ABFU76_RS05180 (ABFU76_05170) - 1235379..1236464 (+) 1086 WP_407463960.1 glycosyltransferase family 9 protein -

Sequence

Protein


Download         Length: 135 a.a.        Molecular weight: 13751.97 Da        Isoelectric Point: 8.8610

>NTDB_id=1000631 ABFU76_RS05160 WP_228442818.1 1231609..1232016(+) (pilA/pilAI) [Xanthomonas campestris pv. raphani strain CFBP 6580]
MKKQQGFTLIELMIVVAIIAILAAIALPMYQDYVAKAQATAGLAEITPGKTQFEVLVNEGSTPTLAGIGLNSPTSRCSSV
VVAATSITCTLRGNASKITGKTLALSRDATTGVWTCQTGGSGGGLDAKYKPAGCS

Nucleotide


Download         Length: 408 bp        

>NTDB_id=1000631 ABFU76_RS05160 WP_228442818.1 1231609..1232016(+) (pilA/pilAI) [Xanthomonas campestris pv. raphani strain CFBP 6580]
ATGAAGAAGCAGCAAGGCTTTACGCTGATCGAACTGATGATCGTGGTCGCGATCATCGCCATCCTGGCCGCCATCGCGCT
GCCGATGTATCAGGACTATGTTGCCAAGGCACAGGCGACTGCTGGCCTGGCCGAAATCACCCCCGGCAAGACGCAGTTCG
AAGTTCTTGTGAATGAAGGCTCTACCCCTACCCTGGCTGGAATTGGTTTGAACAGCCCAACCTCGCGTTGCAGCAGCGTT
GTAGTAGCCGCAACCAGCATCACTTGCACTTTGAGAGGAAATGCATCCAAGATTACTGGCAAAACCCTTGCTCTTAGTCG
GGACGCTACAACAGGTGTTTGGACCTGCCAAACTGGCGGTTCTGGCGGCGGCTTGGATGCCAAGTACAAGCCCGCCGGTT
GCAGCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-134)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

54.286

100

0.563
  pilA Acinetobacter baumannii strain A118

46.528

100

0.496
  pilA/pilAII Pseudomonas stutzeri DSM 10701

46.377

100

0.474
  pilA2 Legionella pneumophila strain ERS1305867

44.286

100

0.459
  pilA2 Legionella pneumophila str. Paris

43.571

100

0.452
  pilA Pseudomonas aeruginosa PAK

38.065

100

0.437
  pilA Ralstonia pseudosolanacearum GMI1000

35.758

100

0.437
  pilA Vibrio cholerae strain A1552

40.136

100

0.437
  pilA Vibrio cholerae C6706

40.136

100

0.437
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

40.136

100

0.437
  pilA/pilA1 Eikenella corrodens VA1

36.667

100

0.407
  pilE Neisseria gonorrhoeae MS11

35.484

100

0.407
  comP Acinetobacter baylyi ADP1

36.184

100

0.407
  pilA Vibrio parahaemolyticus RIMD 2210633

44.628

89.63

0.4
  pilE Neisseria gonorrhoeae strain FA1090

35.294

100

0.4

Multiple sequence alignment