Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ABFU02_RS18115 Genome accession   NZ_CP155974
Coordinates   4206285..4207418 (+) Length   377 a.a.
NCBI ID   WP_115000054.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain 0915     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4201285..4212418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU02_RS18090 (ABFU02_18080) - 4201461..4202660 (-) 1200 WP_228958104.1 cystathionine gamma-synthase -
  ABFU02_RS18095 (ABFU02_18085) - 4202754..4204124 (-) 1371 WP_228958105.1 pyridoxal-phosphate dependent enzyme -
  ABFU02_RS18100 (ABFU02_18090) - 4204356..4204574 (-) 219 WP_019237985.1 YdcH family protein -
  ABFU02_RS18105 (ABFU02_18095) - 4204916..4205806 (-) 891 WP_228958106.1 hypothetical protein -
  ABFU02_RS18110 (ABFU02_18100) - 4205803..4206186 (-) 384 WP_011035838.1 DUF4398 domain-containing protein -
  ABFU02_RS18115 (ABFU02_18105) pilU 4206285..4207418 (+) 1134 WP_115000054.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU02_RS18120 (ABFU02_18110) - 4207681..4208589 (-) 909 WP_228957920.1 transposase -
  ABFU02_RS18125 (ABFU02_18115) maiA 4209156..4209818 (-) 663 WP_228876943.1 maleylacetoacetate isomerase -
  ABFU02_RS18130 (ABFU02_18120) - 4209862..4210851 (-) 990 WP_228957919.1 fumarylacetoacetate hydrolase family protein -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41809.14 Da        Isoelectric Point: 6.1401

>NTDB_id=1000559 ABFU02_RS18115 WP_115000054.1 4206285..4207418(+) (pilU) [Xanthomonas campestris pv. raphani strain 0915]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERELELNMAIALPDAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLVVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHAFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGLDGRRRPATEVLLNTPMIRDLLRRGQVHEVKQAMEESLEEGMQTFDQCL
FRMVKTGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYESASAPKISHGFI

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=1000559 ABFU02_RS18115 WP_115000054.1 4206285..4207418(+) (pilU) [Xanthomonas campestris pv. raphani strain 0915]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTCTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTTTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCTTACTCAT
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGAGCTGGAACTCAACATGGCCATCGCCCTGCCCGACGCCGGGCGC
TTCCGCGTGAACGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTGATCCGTGCCATCCGCAGCCGGATCCCGAGCATCGA
GGAGCTCAACCTGCCGCAGGTGCTCAAGGACGTCATCATGACCCCGCGCGGGCTGGTGCTGGTGGTCGGTTCGACCGGCT
CCGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACGACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAGTACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAGGTGGGCCTGGACACGCACGCCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGACGCCGAAACCATGGAGGCGG
CGATCGCCTTCGCCGAAACCGGTCACCTGTGCCTGGCGACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAAGTGCGCATAAGAACGTGCTGATGAACCTGGCGCTGAATCTGCGCGCAGTGGTGTCCCAGCG
CCTGGTCAAGGGCCTGGACGGGCGCCGCCGCCCGGCCACCGAGGTGCTGCTCAACACGCCGATGATCCGCGACCTGCTAC
GTCGCGGGCAGGTGCACGAGGTCAAGCAGGCGATGGAGGAGTCGCTGGAAGAAGGCATGCAGACCTTCGACCAGTGCCTG
TTCCGGATGGTCAAGACCGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCGGCCGATTCGCGCGATGGCCTGGCGCTGAA
ATTCCGTCTTTCCGAAGGCGGCTCCGGCGAGCACGATCCGTACGCCGACTACGAGTCGGCCTCGGCGCCGAAGATCAGCC
ATGGGTTTATTTAG

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(81-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.368

95.225

0.499
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.764

100

0.454
  pilT Pseudomonas aeruginosa PAK

42.899

89.655

0.385
  pilT Acinetobacter baumannii strain A118

41.42

89.655

0.371
  pilT Acinetobacter nosocomialis M2

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.42

89.655

0.371
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Pseudomonas stutzeri DSM 10701

41.124

89.655

0.369
  pilT Acinetobacter baylyi ADP1

40.882

90.186

0.369
  pilT Vibrio cholerae strain A1552

40.841

88.329

0.361
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

40.841

88.329

0.361
  pilT Neisseria gonorrhoeae MS11

39.193

92.042

0.361
  pilT Neisseria meningitidis 8013

39.193

92.042

0.361

Multiple sequence alignment