Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABFU35_RS06815 Genome accession   NZ_CP155951
Coordinates   1612070..1613107 (+) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. campestris strain bglFP 6813     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1607070..1618107
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU35_RS06785 (ABFU35_06785) - 1607508..1608047 (-) 540 WP_019237453.1 hypothetical protein -
  ABFU35_RS06790 (ABFU35_06790) - 1608101..1608409 (-) 309 WP_019237454.1 DUF3861 family protein -
  ABFU35_RS06795 (ABFU35_06795) - 1608502..1609092 (-) 591 WP_029628896.1 M23 family metallopeptidase -
  ABFU35_RS06800 (ABFU35_06800) - 1609236..1609658 (-) 423 WP_011037886.1 HU family DNA-binding protein -
  ABFU35_RS06805 (ABFU35_06805) proC 1610198..1611058 (-) 861 WP_050912119.1 pyrroline-5-carboxylate reductase -
  ABFU35_RS06810 (ABFU35_06810) - 1611099..1611782 (-) 684 WP_050912120.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFU35_RS06815 (ABFU35_06815) pilT 1612070..1613107 (+) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFU35_RS06820 (ABFU35_06820) pilU 1613230..1614360 (+) 1131 WP_075287180.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU35_RS06825 (ABFU35_06825) - 1614640..1615314 (+) 675 WP_014508435.1 YitT family protein -
  ABFU35_RS06830 (ABFU35_06830) - 1615328..1616227 (-) 900 WP_019237456.1 DUF72 domain-containing protein -
  ABFU35_RS06835 (ABFU35_06835) - 1616450..1617712 (-) 1263 WP_075288043.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=1000150 ABFU35_RS06815 WP_003489919.1 1612070..1613107(+) (pilT) [Xanthomonas campestris pv. campestris strain bglFP 6813]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1000150 ABFU35_RS06815 WP_003489919.1 1612070..1613107(+) (pilT) [Xanthomonas campestris pv. campestris strain bglFP 6813]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCCTTGGACCATAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCGCGT
TTCCGCGTCAATGCGTTCAATCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCGTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTTCGCCAGTTGATCGACCAACCGCAGGGTCTGATACTGGTGACCGGCCCGACCGGTT
CGGGCAAGTCGACCACGCTTGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAT
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAATCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCGCTGCGTTCGGCCTTGCGCGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGATCTGGAAACCATCCGCCTGG
CGCTCACCGCCGCGGAAACCGGCCATCTGGTGTTCGGCACCTTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGTGCGGTGATTTCGCAGGC
ATTGCTCAAGAAAGTGGGCGGCGGGCGTACCGCAGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment