Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABFU60_RS06610 Genome accession   NZ_CP155949
Coordinates   1576401..1577447 (+) Length   348 a.a.
NCBI ID   WP_348882332.1    Uniprot ID   -
Organism   Xanthomonas campestris pv. raphani strain bglFP 6815     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1571401..1582447
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFU60_RS06580 (ABFU60_06575) - 1571840..1572379 (-) 540 WP_070690342.1 hypothetical protein -
  ABFU60_RS06585 (ABFU60_06580) - 1572433..1572741 (-) 309 WP_016944107.1 DUF3861 domain-containing protein -
  ABFU60_RS06590 (ABFU60_06585) - 1572834..1573424 (-) 591 WP_274299438.1 M23 family metallopeptidase -
  ABFU60_RS06595 (ABFU60_06590) - 1573568..1573990 (-) 423 WP_011037886.1 HU family DNA-binding protein -
  ABFU60_RS06600 (ABFU60_06595) proC 1574530..1575390 (-) 861 WP_014508437.1 pyrroline-5-carboxylate reductase -
  ABFU60_RS06605 (ABFU60_06600) - 1575431..1576114 (-) 684 WP_274299439.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABFU60_RS06610 (ABFU60_06605) pilT 1576401..1577447 (+) 1047 WP_348882332.1 type IV pilus twitching motility protein PilT Machinery gene
  ABFU60_RS06615 (ABFU60_06610) pilU 1577570..1578700 (+) 1131 WP_005990431.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABFU60_RS06620 (ABFU60_06615) - 1578980..1579654 (+) 675 WP_014508435.1 YitT family protein -
  ABFU60_RS06625 (ABFU60_06620) - 1579668..1580567 (-) 900 WP_274299440.1 DUF72 domain-containing protein -
  ABFU60_RS06630 (ABFU60_06625) - 1580790..1582052 (-) 1263 WP_274299441.1 hypothetical protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38898.57 Da        Isoelectric Point: 6.6652

>NTDB_id=1000114 ABFU60_RS06610 WP_348882332.1 1576401..1577447(+) (pilT) [Xanthomonas campestris pv. raphani strain bglFP 6815]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGPTGSGKSTTLAGMIDYINKNEYGHILT
VEDPIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTI
DRIIDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLD
QHLQDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=1000114 ABFU60_RS06610 WP_348882332.1 1576401..1577447(+) (pilT) [Xanthomonas campestris pv. raphani strain bglFP 6815]
ATGGATATCGCTGAACTATTGGCGTTTTCTGTCAAGAACAAGGCATCGGACCTGCACCTGTCTGCAGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGCCGCATCAATATTCCGGCCTTGGACCATAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCGCGT
TTCCGCGTCAATGCGTTCAATCAGAACCGTGGCGCCGGTGCGGTGTTCCGTACCATTCCCTCCGAAGTGCTGACGCTGGA
AGACCTGGGCTGCCCGCCGATCTTTCGCCAGTTGATCGACCAGCCGCAGGGTTTGATCCTGGTGACCGGCCCGACCGGCC
CGACCGGGTCGGGCAAGTCGACCACGCTTGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACC
GTCGAGGATCCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGG
CTTCAACGAGGCGCTGCGTTCGGCCTTGCGTGAAGACCCGGACATCATCCTGGTCGGCGAGTTGCGCGATCTGGAAACCA
TCCGCCTGGCACTGACCGCCGCGGAAACCGGCCATCTGGTGTTCGGCACCTTGCACACCAGCTCGGCGGCCAAGACCATC
GACCGCATCATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAATCGCTGCGCGCGGTGAT
TTCGCAGGCATTGCTCAAGAAAGTGGGCGGCGGGCGTACCGCAGCATGGGAAATCATGGTCGGCACCCCGGCCATCCGCA
ACCTGATCCGCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGCATGCAGACGCTGGAC
CAGCATCTGCAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCGCGCGAGTACGCCAAGGACAAGCGGATATT
CGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.251

99.713

0.79
  pilT Acinetobacter baumannii strain A118

79.251

99.713

0.79
  pilT Acinetobacter nosocomialis M2

79.251

99.713

0.79
  pilT Pseudomonas stutzeri DSM 10701

78.674

99.713

0.784
  pilT Acinetobacter baylyi ADP1

78.098

99.713

0.779
  pilT Pseudomonas aeruginosa PAK

78.363

98.276

0.77
  pilT Legionella pneumophila strain Lp02

74.64

99.713

0.744
  pilT Legionella pneumophila strain ERS1305867

74.64

99.713

0.744
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.482

95.402

0.672
  pilT Vibrio cholerae strain A1552

70.482

95.402

0.672
  pilT Neisseria meningitidis 8013

65.407

98.851

0.647
  pilT Neisseria gonorrhoeae MS11

65.116

98.851

0.644
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.425

100

0.494
  pilU Pseudomonas stutzeri DSM 10701

40.351

98.276

0.397
  pilU Acinetobacter baylyi ADP1

39.942

98.563

0.394
  pilU Vibrio cholerae strain A1552

40.237

97.126

0.391

Multiple sequence alignment