Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
| T6SS ID | T6SS00292  |
| T6SS type | Type i4b |
| Strain | Burkholderia cenocepacia J2315 |
| Replicon (RefSeq) | chromosome I (NC_011000) |
| Location | 363015..382880 |
| Function | anti-eukaryotic; effector translocation; virulence |
| Comment | Resistance to Dictyostelium predation; virulence in rat model of chronic lung infection; induction of cytoskeletal rearrangements in cultured macrophages; inflammasome activation. |
| References | [1] O'Grady EP et al (2009) Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia. BMC Genomics. 0.722916667. [PudMed:19761612] [2] Aubert D et al (2010) BcsKC is an essential protein for the type VI secretion system activity in Burkholderia cenocepacia that forms an outer membrane complex with BcsLB. J Biol Chem. 285(46):35988-98. [PudMed:20729192] [3] Rosales-Reyes R et al (2012) The suhB gene of Burkholderia cenocepacia is required for protein secretion, biofilm formation, motility and polymyxin B resistance. Microbiology. 158(Pt 9):2315-24. [PudMed:22767545] [4] Khodai-Kalaki M et al (2013) Characterization of the AtsR hybrid sensor kinase phosphorelay pathway and identification of its response regulator in Burkholderia cenocepacia. J Biol Chem. 288(42):30473-84. [PudMed:24014026] [5] Tolman JS et al (2012) Global changes in gene expression by the opportunistic pathogen Burkholderia cenocepacia in response to internalization by murine macrophages. BMC Genomics. 0.585416667. [PudMed:22321740] [6] Aubert DF et al (2013) The Burkholderia cenocepacia sensor kinase hybrid AtsR is a global regulator modulating quorum-sensing signalling. Environ Microbiol. 15(2):372-85. [PudMed:22830644] [7] Flannagan RS et al (2012) Burkholderia cenocepacia disrupts host cell actin cytoskeleton by inactivating Rac and Cdc42. Cell Microbiol. 14(2):239-54. [PudMed:22023324] [8] Rosales-Reyes R et al (2012) Burkholderia cenocepacia Type VI Secretion System Mediates Escape of Type II Secreted Proteins into the Cytoplasm of Infected Macrophages. PLoS One. 7(7):e41726. [PudMed:22848580] [9] Xu H et al (2014) Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome. Nature. 513(7517):237-41. [PudMed:24919149] |
Experimental investigation has been performed.T6SS components and genome coordinates
| Component | TagL | TssA | TssB | TssC | TssD | TssE | TssF | TssG | TssH | TssJ | TssK | TssL | TssM |
| Number | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
| Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
|---|---|---|---|---|---|---|---|
| QU43_RS38180 | 358266..359024 | + | 759 | WP_006477108.1 | cytochrome c1 | ||
| QU43_RS38185 | 359119..359730 | + | 612 | WP_006400565.1 | glutathione S-transferase N-terminal domain-containing protein | ||
| QU43_RS38190 | 359802..360323 | + | 522 | WP_006485328.1 | ClpXP protease specificity-enhancing factor | ||
| QU43_RS38200 | 360772..360975 | + | 204 | WP_006485326.1 | hypothetical protein | ||
| QU43_RS38205 | 361085..361885 | - | 801 | WP_006485337.1 | transporter substrate-binding domain-containing protein | ||
| QU43_RS38210 | 362167..362484 | + | 318 | WP_006485338.1 | hypothetical protein | ||
| QU43_RS38215 | 362566..362901 | + | 336 | WP_006485334.1 | hypothetical protein | ||
| T6CP002953 | TssL | QU43_RS38220 | 363015..363797 | - | 783 | WP_006491869.1 | type IVB secretion system protein IcmH/DotU |
| T6CP002954 | TssK | QU43_RS38225 (tssK) | 363794..365140 | - | 1347 | WP_006477098.1 | type VI secretion system baseplate subunit TssK |
| T6CP002955 | TssJ | QU43_RS38230 (tssJ) | 365246..365845 | - | 600 | WP_006485335.1 | type VI secretion system lipoprotein TssJ |
| QU43_RS38235 | 366232..366858 | + | 627 | WP_012492309.1 | hypothetical protein | ||
| T6CP002956 | TssB | QU43_RS38240 (tssB) | 366905..367420 | + | 516 | WP_006484395.1 | type VI secretion system contractile sheath small subunit |
| T6CP002957 | TssC | QU43_RS38245 (tssC) | 367436..368926 | + | 1491 | WP_006484397.1 | type VI secretion system contractile sheath large subunit |
| T6CP002958 | TssD | QU43_RS38250 | 368997..369500 | + | 504 | WP_006477093.1 | type VI secretion system tube protein Hcp |
| T6CP002959 | TssE | QU43_RS38255 (tssE) | 369563..370048 | + | 486 | WP_006477092.1 | type VI secretion system baseplate subunit TssE |
| T6CP002960 | TssF | QU43_RS38260 (tssF) | 370125..371960 | + | 1836 | WP_006484396.1 | type VI secretion system baseplate subunit TssF |
| T6CP002961 | TssG | QU43_RS38265 (tssG) | 371924..373024 | + | 1101 | WP_006484394.1 | type VI secretion system baseplate subunit TssG |
| T6CP002962 | TssH | QU43_RS38270 (tssH) | 373066..375735 | + | 2670 | WP_006484392.1 | type VI secretion system ATPase TssH |
| T6CP002963 | TssA | QU43_RS38275 (tssA) | 375780..376901 | + | 1122 | WP_006484398.1 | type VI secretion system protein TssA |
| T6CP011387 | TagL | QU43_RS38280 | 376995..377945 | - | 951 | WP_006494417.1 | OmpA family protein |
| QU43_RS38285 (tagF) | 377950..378939 | - | 990 | WP_006483243.1 | type VI secretion system-associated protein TagF | ||
| T6CP002964 | TssM | QU43_RS38290 (tssM) | 378936..382880 | - | 3945 | WP_012492310.1 | type VI secretion system membrane subunit TssM |
| QU43_RS38295 | 383182..384027 | - | 846 | WP_006488977.1 | M15 family metallopeptidase | ||
| QU43_RS38300 | 384149..385141 | + | 993 | WP_012492311.1 | hypothetical protein | ||
| QU43_RS38305 | 385353..386381 | + | 1029 | WP_006481924.1 | transporter | ||
| QU43_RS73250 | 386409..386561 | - | 153 | WP_006481917.1 | DUF1427 family protein | ||
| QU43_RS38310 | 386586..387563 | - | 978 | WP_012492312.1 | zinc-binding alcohol dehydrogenase family protein |
Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.| Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
|---|
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Effector ID | Locus tag (Gene) | Classification | Cognate immunity protein | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|---|---|---|---|---|---|---|
| EFF00353 |
QU43_RS38250 | - | - | Burkholderia cenocepacia J2315 | chromosome (NC_011000) | 368997..369500 (+) | 206558749 |
Experimental investigation has been performed. Bioinformatics investigation has been performed.| Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
|---|
Experimental investigation has been performed.| Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
|---|