Detailed information of component protein
Summary
| Component ID |
T6CP002958 |
| Component protein type |
TssD |
| T6SS ID (Type) |
T6SS00292  (Type i4b) |
| Strain |
Burkholderia cenocepacia J2315 |
| Replicon |
chromosome I |
| Sequence |
Protein sequence (168 a.a.); Nucleotide sequence (504 bp) |
| Reference |
[1] Rosales-Reyes R, Aubert DF, Tolman JS, et al. Burkholderia cenocepacia type VI secretion system mediates escape of type II secreted proteins into the cytoplasm of infected macrophages. PLoS One. 2012;7(7):e41726. doi: 10.1371/journal.pone.0041726. Epub 2012 Jul 25. PMID: 22848580[2] Tolman JS, Valvano MA. Global changes in gene expression by the opportunistic pathogen Burkholderia cenocepacia in response to internalization by murine macrophages. BMC Genomics. 2012 Feb 9;13:63. doi: 10.1186/1471-2164-13-63. PMID: 22321740[3] Flannagan RS, JaumouillĂ© V, Huynh KK, et al. Burkholderia cenocepacia disrupts host cell actin cytoskeleton by inactivating Rac and Cdc42. Cell Microbiol. 2012 Feb;14(2):239-54. doi: 10.1111/j.1462-5822.2011.01715.x. Epub 2011 Nov 15. PMID: 22023324[4] Aubert D, MacDonald DK, Valvano MA. BcsKC is an essential protein for the type VI secretion system activity in Burkholderia cenocepacia that forms an outer membrane complex with BcsLB. J Biol Chem. 2010 Nov 12;285(46):35988-98. doi: 10.1074/jbc.M110.120402. Epub 2010 Aug 20. PMID: 20729192[5] Rosales-Reyes R, SaldĂas MS, Aubert DF, et al. The suhB gene of Burkholderia cenocepacia is required for protein secretion, biofilm formation, motility and polymyxin B resistance. Microbiology (Reading). 2012 Sep;158(Pt 9):2315-2324. doi: 10.1099/mic.0.060988-0. Epub 2012 Jul 5. PMID: 22767545[6] Aubert DF, O'Grady EP, Hamad MA, et al. The Burkholderia cenocepacia sensor kinase hybrid AtsR is a global regulator modulating quorum-sensing signalling. Environ Microbiol. 2013 Feb;15(2):372-85. doi: 10.1111/j.1462-2920.2012.02828.x. Epub 2012 Jul 26. PMID: 22830644[7] Khodai-Kalaki M, Aubert DF, Valvano MA. Characterization of the AtsR hybrid sensor kinase phosphorelay pathway and identification of its response regulator in Burkholderia cenocepacia. J Biol Chem. 2013 Oct 18;288(42):30473-30484. doi: 10.1074/jbc.M113.489914. Epub 2013 Sep 6. PMID: 24014026[8] Xu H, Yang J, Gao W, et al. Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome. Nature. 2014 Sep 11;513(7517):237-41. doi: 10.1038/nature13449. Epub 2014 Jun 11. PMID: 24919149[9] O'Grady EP, Viteri DF, Malott RJ, et al. Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia. BMC Genomics. 2009 Sep 17;10:441. doi: 10.1186/1471-2164-10-441. PMID: 19761612 |
Experimental investigation has been performed on this T6SS
External database links
| Locus tag (Gene) |
QU43_RS38250 |
| Coordinate (Strand) |
368997..369500 (+) |
| NCBI ID |
WP_006477093.1 |
| RefSeq |
NC_011000 |
| Uniprot ID |
J7JDR9_BURCE, A0A0B1YQS5_9BURK, A0A0U4DCL3_BURCE, A0A4S5CPF0_9BURK, A0A6J5EX57_9BURK, A0A084D9C9_9BURK, A0A2S9KZ71_9BURK, A0A1K1S9E0_9BURK, A0A4V8GCX1_9BURK, A0A117VGM3_9BURK, A0A1B4F605_9BURK, A0A103LBA0_9BURK, A0A2A4CEA2_9BURK, A0A6P2GAQ5_9BURK, A0A1X1P7H9_9BURK, A0A2A9LW14_9BURK, A0A228PYE3_9BURK, A0A228HBG8_9BURK, A0A228I0N3_9BURK, A0A228J4P0_9BURK, A0A228K684_9BURK, A0A228RK00_9BURK, A0A228QKE8_9BURK, A0A4R4BX44_9BURK, A0A495YLN9_9BURK, B4E6R5_BURCJ, A0A364GSY9_9BURK, A0A3N8GU54_9BURK, A0A3P0S1M1_9BURK, A0A3N8D151_9BURK, A0A3P0NX81_9BURK, A0A3P0MQB7_9BURK, A0A3N8C350_9BURK, A0A3N7ZH20_9BURK, A0A3N7ZEL0_9BURK, A0A2U9SGD4_9BURK, A0A365QNQ2_9BURK, A0A7C9FHE2_9BURK, A0A081V0J2_BURCE, A0A0H2XRS0_BURCA, Q0BIT1_BURCM, A2VTE0_9BURK, B1FDD0_9BURK, B1T015_9BURK, B1YS99_BURA4, B1JUN9_BURCC, Q39K54_BURL3, A0A0G3YXS0_9BURK, A0A1B4HQA6_9BURK, A0A6P2PVE7_9BURK, A0A1B4BP34_9BURK, A0A087NNS3_BURPY, A0A1D7ZEA5_9BURK, A0A0M1IMJ7_9BURK |
| KEGG ID |
bcon:NL30_20125, bct:GEM_3042, bcn:Bcen_2637, bcm:Bcenmc03_0447, bdf:WI26_01920, bstl:BBJ41_12130, bur:Bcep18194_A3561, bam:Bamb_0383, bced:DM42_1364, bcj:BCAL0343, bpyr:ABD05_07980, bmec:WJ16_01965, bac:BamMC406_0404 |
| PDB ID |
- |
Pfam domain hit(s)
| Domain |
Pfam ID |
E-value |
Aligned region |
| T6SS_HCP |
PF05638.14 |
3.1e-37
|
10..138 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction |
Region |
Sequence |
| Outside |
1-167 |
MLHMHLQFGSPAVKGESADKDHQGWIELKSWDHSIVQPRSATASTAGGHTMTRCEHGDMIFTKEIDSSSPLLYQHASGGTTFDEVTVHFSRADGEGKRVQYLEVKLKYVIISSIAPSVREEGLPLETFSLKYAAVQWKQTQQKIGGNQGGNTQGAWSLTKNDKTYAV |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction |
Probability |
Cleavage site |
Signal peptide sequence |
| Other |
0.9447 |
- |
- |
Protein sequence: 168 a.a.
>T6CP002958 NC_011000:368997-369500 [Burkholderia cenocepacia J2315] [TssD]
MLHMHLQFGSPAVKGESADKDHQGWIELKSWDHSIVQPRSATASTAGGHTMTRCEHGDMIFTKEIDSSSPLLYQHASGGT
TFDEVTVHFSRADGEGKRVQYLEVKLKYVIISSIAPSVREEGLPLETFSLKYAAVQWKQTQQKIGGNQGGNTQGAWSLTK
NDKTYAV*
Nucleotide sequence: 504 bp
>T6CP002958 NC_011000:368997-369500 [Burkholderia cenocepacia J2315] [TssD]
ATGTTACATATGCACTTGCAGTTTGGTAGTCCCGCGGTGAAGGGCGAATCCGCGGACAAGGACCATCAGGGCTGGATCGA
ACTGAAATCGTGGGATCACTCGATCGTTCAGCCGCGTTCGGCAACCGCATCGACCGCTGGCGGTCACACGATGACGCGTT
GCGAGCACGGCGACATGATCTTCACGAAGGAAATCGATTCGTCGAGCCCGCTGCTGTACCAGCACGCTTCGGGCGGTACC
ACGTTCGACGAAGTGACGGTCCATTTCTCGCGCGCGGACGGTGAAGGCAAGCGCGTGCAGTATCTGGAAGTCAAGCTCAA
ATACGTGATCATCTCGAGCATCGCCCCGAGCGTCCGCGAGGAAGGTCTGCCCCTCGAGACGTTCTCGCTGAAGTACGCAG
CCGTGCAGTGGAAGCAAACCCAGCAGAAGATCGGCGGCAACCAGGGCGGCAACACGCAAGGCGCCTGGAGCCTGACGAAG
AACGACAAGACCTACGCGGTCTAA