Detailed information of component protein
Summary
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | QU43_RS38280 |
| Coordinate (Strand) | 376995..377945 (-) |
| NCBI ID | WP_006494417.1 |
| RefSeq | NC_011000 |
| Uniprot ID | B4E6S1_BURCJ, A0A364GT47_9BURK, A0A144RZ74_9BURK |
| KEGG ID | bcj:BCAL0349 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| OmpA | PF00691.22 | 1.1e-22 | 214..308 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-11 | MHRTIQVRRAR |
| Transmembrane helix | 12-34 | LSAAVSPALAALFAAGLVASGAA |
| Outside | 35-316 | FAQNTGATVTPVGNGTVATTALPQANPNAGGASGTVVHGTAGTLTPPPANAAPGQVVVGGKVPDEATKAAVLQKLRDTYGATNVVDQIEVGDVATPPNWSANVQKLLGAQLKQISKGQLKINGTQIEMKGEVHNEAQRQQLASDMANTLNPTYTIKNGLRVSASEQGVLDQTLANRTIEFETGSATLTPQGKLILDQMAAALAKMQNRTVDIIGHTDNSGNRTSNIALSQARADAVKGYLITKSIPPQQMTTTGVGPDQPIAPNDTADGRARNRRIEFRVGQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| SP(Sec/SPI) | 0.8246 | pos: 36-37. AFA-QN. | MHRTIQVRRARLSAAVSPALAALFAAGLVASGAAFA |
Protein sequence: 317 a.a.
>T6CP011387 NC_011000:c377945-376995 [Burkholderia cenocepacia J2315] [TagL]
MHRTIQVRRARLSAAVSPALAALFAAGLVASGAAFAQNTGATVTPVGNGTVATTALPQANPNAGGASGTVVHGTAGTLTP
PPANAAPGQVVVGGKVPDEATKAAVLQKLRDTYGATNVVDQIEVGDVATPPNWSANVQKLLGAQLKQISKGQLKINGTQI
EMKGEVHNEAQRQQLASDMANTLNPTYTIKNGLRVSASEQGVLDQTLANRTIEFETGSATLTPQGKLILDQMAAALAKMQ
NRTVDIIGHTDNSGNRTSNIALSQARADAVKGYLITKSIPPQQMTTTGVGPDQPIAPNDTADGRARNRRIEFRVGQ*
MHRTIQVRRARLSAAVSPALAALFAAGLVASGAAFAQNTGATVTPVGNGTVATTALPQANPNAGGASGTVVHGTAGTLTP
PPANAAPGQVVVGGKVPDEATKAAVLQKLRDTYGATNVVDQIEVGDVATPPNWSANVQKLLGAQLKQISKGQLKINGTQI
EMKGEVHNEAQRQQLASDMANTLNPTYTIKNGLRVSASEQGVLDQTLANRTIEFETGSATLTPQGKLILDQMAAALAKMQ
NRTVDIIGHTDNSGNRTSNIALSQARADAVKGYLITKSIPPQQMTTTGVGPDQPIAPNDTADGRARNRRIEFRVGQ*
Nucleotide sequence: 951 bp
>T6CP011387 NC_011000:c377945-376995 [Burkholderia cenocepacia J2315] [TagL]
ATGCATCGCACGATTCAGGTCCGGCGCGCGCGCTTGTCGGCCGCCGTCTCGCCCGCCCTCGCCGCGCTGTTCGCGGCCGG
CCTCGTCGCGAGCGGCGCGGCCTTCGCGCAGAACACCGGCGCGACCGTCACGCCGGTCGGCAACGGCACGGTCGCGACGA
CCGCGCTGCCGCAGGCCAACCCCAACGCCGGCGGCGCGTCCGGCACGGTGGTGCACGGCACCGCCGGCACGCTCACGCCG
CCGCCCGCGAACGCGGCGCCCGGCCAGGTGGTGGTCGGCGGCAAGGTGCCCGACGAGGCGACCAAGGCCGCCGTGCTGCA
GAAGCTGCGCGACACCTACGGCGCGACGAACGTCGTCGACCAGATCGAGGTGGGCGACGTCGCGACGCCGCCGAACTGGA
GCGCGAACGTGCAGAAACTGCTCGGCGCGCAGCTCAAGCAGATCAGCAAGGGCCAGTTGAAGATCAACGGCACGCAGATC
GAGATGAAGGGCGAGGTGCACAACGAGGCGCAGCGCCAGCAGCTCGCGAGCGACATGGCGAACACGCTGAACCCGACGTA
CACGATCAAGAACGGGCTGCGCGTGTCGGCGTCCGAGCAGGGCGTGCTCGACCAGACGCTCGCGAACCGGACGATCGAGT
TCGAGACCGGCAGCGCGACGCTGACGCCGCAGGGCAAGTTGATCCTCGACCAGATGGCGGCCGCGCTCGCGAAGATGCAG
AACCGCACGGTCGACATCATCGGCCACACCGACAACTCGGGGAACCGGACGTCGAACATCGCGCTGAGCCAGGCGCGCGC
GGATGCGGTGAAGGGCTACCTGATCACGAAGAGCATTCCGCCGCAGCAGATGACGACGACGGGCGTCGGGCCGGATCAGC
CGATCGCGCCGAACGATACGGCCGACGGGCGGGCGCGGAACCGGCGGATCGAATTCCGCGTCGGGCAGTAA
ATGCATCGCACGATTCAGGTCCGGCGCGCGCGCTTGTCGGCCGCCGTCTCGCCCGCCCTCGCCGCGCTGTTCGCGGCCGG
CCTCGTCGCGAGCGGCGCGGCCTTCGCGCAGAACACCGGCGCGACCGTCACGCCGGTCGGCAACGGCACGGTCGCGACGA
CCGCGCTGCCGCAGGCCAACCCCAACGCCGGCGGCGCGTCCGGCACGGTGGTGCACGGCACCGCCGGCACGCTCACGCCG
CCGCCCGCGAACGCGGCGCCCGGCCAGGTGGTGGTCGGCGGCAAGGTGCCCGACGAGGCGACCAAGGCCGCCGTGCTGCA
GAAGCTGCGCGACACCTACGGCGCGACGAACGTCGTCGACCAGATCGAGGTGGGCGACGTCGCGACGCCGCCGAACTGGA
GCGCGAACGTGCAGAAACTGCTCGGCGCGCAGCTCAAGCAGATCAGCAAGGGCCAGTTGAAGATCAACGGCACGCAGATC
GAGATGAAGGGCGAGGTGCACAACGAGGCGCAGCGCCAGCAGCTCGCGAGCGACATGGCGAACACGCTGAACCCGACGTA
CACGATCAAGAACGGGCTGCGCGTGTCGGCGTCCGAGCAGGGCGTGCTCGACCAGACGCTCGCGAACCGGACGATCGAGT
TCGAGACCGGCAGCGCGACGCTGACGCCGCAGGGCAAGTTGATCCTCGACCAGATGGCGGCCGCGCTCGCGAAGATGCAG
AACCGCACGGTCGACATCATCGGCCACACCGACAACTCGGGGAACCGGACGTCGAACATCGCGCTGAGCCAGGCGCGCGC
GGATGCGGTGAAGGGCTACCTGATCACGAAGAGCATTCCGCCGCAGCAGATGACGACGACGGGCGTCGGGCCGGATCAGC
CGATCGCGCCGAACGATACGGCCGACGGGCGGGCGCGGAACCGGCGGATCGAATTCCGCGTCGGGCAGTAA