Detailed information of component protein


Summary

Component ID T6CP002961
Component protein type TssG
T6SS ID (Type) T6SS00292 experimental (Type i4b)
Strain Burkholderia cenocepacia J2315
Replicon chromosome I
Sequence Protein sequence (367 a.a.); Nucleotide sequence (1101 bp)
Reference
[1] Rosales-Reyes R, Aubert DF, Tolman JS, et al. Burkholderia cenocepacia type VI secretion system mediates escape of type II secreted proteins into the cytoplasm of infected macrophages. PLoS One. 2012;7(7):e41726. doi: 10.1371/journal.pone.0041726. Epub 2012 Jul 25. PMID: 22848580
[2] Tolman JS, Valvano MA. Global changes in gene expression by the opportunistic pathogen Burkholderia cenocepacia in response to internalization by murine macrophages. BMC Genomics. 2012 Feb 9;13:63. doi: 10.1186/1471-2164-13-63. PMID: 22321740
[3] Flannagan RS, Jaumouillé V, Huynh KK, et al. Burkholderia cenocepacia disrupts host cell actin cytoskeleton by inactivating Rac and Cdc42. Cell Microbiol. 2012 Feb;14(2):239-54. doi: 10.1111/j.1462-5822.2011.01715.x. Epub 2011 Nov 15. PMID: 22023324
[4] Aubert D, MacDonald DK, Valvano MA. BcsKC is an essential protein for the type VI secretion system activity in Burkholderia cenocepacia that forms an outer membrane complex with BcsLB. J Biol Chem. 2010 Nov 12;285(46):35988-98. doi: 10.1074/jbc.M110.120402. Epub 2010 Aug 20. PMID: 20729192
[5] Rosales-Reyes R, SaldĂ­as MS, Aubert DF, et al. The suhB gene of Burkholderia cenocepacia is required for protein secretion, biofilm formation, motility and polymyxin B resistance. Microbiology (Reading). 2012 Sep;158(Pt 9):2315-2324. doi: 10.1099/mic.0.060988-0. Epub 2012 Jul 5. PMID: 22767545
[6] Aubert DF, O'Grady EP, Hamad MA, et al. The Burkholderia cenocepacia sensor kinase hybrid AtsR is a global regulator modulating quorum-sensing signalling. Environ Microbiol. 2013 Feb;15(2):372-85. doi: 10.1111/j.1462-2920.2012.02828.x. Epub 2012 Jul 26. PMID: 22830644
[7] Khodai-Kalaki M, Aubert DF, Valvano MA. Characterization of the AtsR hybrid sensor kinase phosphorelay pathway and identification of its response regulator in Burkholderia cenocepacia. J Biol Chem. 2013 Oct 18;288(42):30473-30484. doi: 10.1074/jbc.M113.489914. Epub 2013 Sep 6. PMID: 24014026
[8] Xu H, Yang J, Gao W, et al. Innate immune sensing of bacterial modifications of Rho GTPases by the Pyrin inflammasome. Nature. 2014 Sep 11;513(7517):237-41. doi: 10.1038/nature13449. Epub 2014 Jun 11. PMID: 24919149
[9] O'Grady EP, Viteri DF, Malott RJ, et al. Reciprocal regulation by the CepIR and CciIR quorum sensing systems in Burkholderia cenocepacia. BMC Genomics. 2009 Sep 17;10:441. doi: 10.1186/1471-2164-10-441. PMID: 19761612
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) QU43_RS38265
Coordinate (Strand) 371924..373024 (+)
NCBI ID WP_006484394.1
RefSeq NC_011000
Uniprot ID B4E6R8_BURCJ, A0A1V6KPS4_9BURK
KEGG ID bcj:BCAL0346
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssG PF06996.13 6.1e-90 19..346
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-366 MQAPNRRIDPGVVDALLDEPHRFEFFQAVRVLEGLFARQASDAPGAWRQGDVVAQRIAFRNTLSLGFPPSEIEGARSFDDDGASLSSDEQRGAALAAGELGRVELTPAFFGLLGGQGALPLHYTEQIVAREHLRRDHAARAFFDVFSNRATALFYAAWKKYRLPFHYELDRDERYLPLLLAIAGVPSDEVRDSLAAGAGGVLDEAVAGYALAARHRPMSAAYLQRTLSDYFRVPVKIDQFVGKWYDVPPDQLSVLGEVNAVLGATALVGERVWQRDMRARIVVGPLSKRDYEAFLPGGAQAVALERMLTLLAGVTLEYEVKLVLKRTEVGASVLGAGSRLGWDAFLCTRDAAEDRSDARYELHVIH


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9826 - -


  Protein sequence: 367 a.a.    

>T6CP002961 NC_011000:371924-373024 [Burkholderia cenocepacia J2315] [TssG]
MQAPNRRIDPGVVDALLDEPHRFEFFQAVRVLEGLFARQASDAPGAWRQGDVVAQRIAFRNTLSLGFPPSEIEGARSFDD
DGASLSSDEQRGAALAAGELGRVELTPAFFGLLGGQGALPLHYTEQIVAREHLRRDHAARAFFDVFSNRATALFYAAWKK
YRLPFHYELDRDERYLPLLLAIAGVPSDEVRDSLAAGAGGVLDEAVAGYALAARHRPMSAAYLQRTLSDYFRVPVKIDQF
VGKWYDVPPDQLSVLGEVNAVLGATALVGERVWQRDMRARIVVGPLSKRDYEAFLPGGAQAVALERMLTLLAGVTLEYEV
KLVLKRTEVGASVLGAGSRLGWDAFLCTRDAAEDRSDARYELHVIH*

  Nucleotide sequence: 1101 bp    

>T6CP002961 NC_011000:371924-373024 [Burkholderia cenocepacia J2315] [TssG]
ATGCAAGCCCCGAACCGGCGAATCGATCCTGGCGTAGTCGACGCGCTGCTCGACGAGCCGCACCGCTTCGAGTTCTTCCA
GGCGGTGCGCGTGCTCGAGGGGCTGTTCGCGCGGCAGGCGTCGGATGCGCCCGGCGCATGGCGGCAGGGCGACGTGGTCG
CGCAGCGCATCGCATTCCGCAACACGCTGTCGCTCGGCTTTCCGCCGAGCGAGATCGAAGGCGCGCGCTCGTTCGACGAC
GACGGCGCGTCGCTGAGTTCGGACGAGCAGCGCGGCGCCGCGCTGGCCGCCGGCGAGCTCGGCCGCGTCGAGCTGACGCC
CGCGTTCTTCGGGCTGCTCGGCGGGCAAGGCGCGCTGCCGCTGCACTACACCGAGCAGATCGTCGCGCGCGAGCATCTGC
GGCGCGACCATGCGGCGCGCGCGTTCTTCGACGTGTTCTCGAACCGCGCGACCGCGCTGTTCTACGCGGCGTGGAAGAAA
TACCGGCTGCCGTTCCACTACGAGCTCGACCGCGACGAGCGCTACCTGCCGCTGTTGCTCGCGATCGCGGGCGTGCCGAG
CGACGAGGTGCGCGACAGCCTCGCGGCCGGCGCGGGCGGTGTGCTCGACGAGGCCGTGGCCGGCTACGCGCTCGCCGCGC
GGCACCGGCCGATGTCGGCCGCCTACCTGCAGCGCACGCTGTCCGATTACTTCCGCGTGCCGGTGAAGATCGACCAGTTC
GTCGGCAAGTGGTACGACGTGCCGCCCGATCAGCTGAGCGTGCTCGGTGAAGTCAACGCGGTGCTCGGCGCGACGGCGCT
CGTCGGCGAGCGCGTGTGGCAGCGCGACATGCGCGCGCGAATCGTCGTCGGCCCGCTGTCCAAGCGCGACTACGAGGCGT
TCCTGCCCGGCGGCGCGCAGGCCGTCGCGCTCGAGCGGATGCTGACGCTGCTCGCCGGCGTCACGCTCGAATACGAGGTG
AAGCTCGTGCTGAAGCGCACCGAGGTCGGCGCGAGCGTGCTCGGCGCGGGCTCGCGGCTCGGCTGGGATGCGTTCCTGTG
CACGCGCGACGCGGCCGAGGACCGTTCGGACGCCCGCTACGAACTGCACGTGATTCACTGA