Detailed information of component protein
Summary
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | QU43_RS38230 |
| Coordinate (Strand) | 365246..365845 (-) |
| NCBI ID | WP_006485335.1 |
| RefSeq | NC_011000 |
| Uniprot ID | UPI00017B90FD |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 8.1e-30 | 50..168 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-4 | MIRY |
| Transmembrane helix | 5-27 | ALPLLACALLAGCAAAPLLGSAA |
| Outside | 28-199 | SAVMSAAGIGKPEVPDAQKPPRNIGLTLAAAPNLNAATDNKPLALVVRLYALKDPTSFQQAPFDAFTDPAKEKTALGADLLNVREITLIPGQRYTATEKVSREAQAFGIVALFRDPALQRWKLTFDPVKSEKSGIIIGLHNCAMTVTDGTVIAPQQGAPSQPLNMLSSVSCG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.8408 | pos: 16-17. LAG-CA. | MIRYALPLLACALLAG |
Protein sequence: 200 a.a.
>T6CP002955 NC_011000:c365845-365246 [Burkholderia cenocepacia J2315] [TssJ]
MIRYALPLLACALLAGCAAAPLLGSAASAVMSAAGIGKPEVPDAQKPPRNIGLTLAAAPNLNAATDNKPLALVVRLYALK
DPTSFQQAPFDAFTDPAKEKTALGADLLNVREITLIPGQRYTATEKVSREAQAFGIVALFRDPALQRWKLTFDPVKSEKS
GIIIGLHNCAMTVTDGTVIAPQQGAPSQPLNMLSSVSCG*
MIRYALPLLACALLAGCAAAPLLGSAASAVMSAAGIGKPEVPDAQKPPRNIGLTLAAAPNLNAATDNKPLALVVRLYALK
DPTSFQQAPFDAFTDPAKEKTALGADLLNVREITLIPGQRYTATEKVSREAQAFGIVALFRDPALQRWKLTFDPVKSEKS
GIIIGLHNCAMTVTDGTVIAPQQGAPSQPLNMLSSVSCG*
Nucleotide sequence: 600 bp
>T6CP002955 NC_011000:c365845-365246 [Burkholderia cenocepacia J2315] [TssJ]
ATGATTCGCTACGCGCTGCCGCTGCTTGCGTGCGCCCTTCTGGCCGGATGCGCGGCCGCTCCGCTGCTCGGCTCGGCCGC
GAGCGCCGTGATGTCCGCGGCCGGCATCGGCAAGCCCGAGGTGCCCGACGCGCAGAAGCCGCCGCGCAACATAGGCCTCA
CGCTGGCCGCCGCACCGAACCTGAATGCCGCGACCGACAACAAGCCGCTTGCGCTCGTCGTGCGCCTCTATGCGCTGAAA
GACCCGACCTCGTTCCAGCAAGCGCCGTTCGACGCATTTACCGACCCGGCCAAGGAAAAAACCGCACTCGGCGCCGATCT
GCTGAACGTGCGTGAAATCACGCTGATTCCGGGCCAGCGCTATACGGCAACCGAGAAGGTATCGCGCGAAGCGCAGGCAT
TCGGCATTGTCGCGCTGTTCCGCGATCCCGCACTGCAACGCTGGAAACTGACGTTCGATCCGGTGAAGTCGGAGAAATCC
GGCATAATCATCGGCCTGCATAATTGCGCGATGACGGTCACCGACGGCACGGTCATCGCACCGCAACAGGGTGCGCCTTC
GCAACCGCTGAATATGCTGTCTTCGGTCTCCTGCGGTTAA
ATGATTCGCTACGCGCTGCCGCTGCTTGCGTGCGCCCTTCTGGCCGGATGCGCGGCCGCTCCGCTGCTCGGCTCGGCCGC
GAGCGCCGTGATGTCCGCGGCCGGCATCGGCAAGCCCGAGGTGCCCGACGCGCAGAAGCCGCCGCGCAACATAGGCCTCA
CGCTGGCCGCCGCACCGAACCTGAATGCCGCGACCGACAACAAGCCGCTTGCGCTCGTCGTGCGCCTCTATGCGCTGAAA
GACCCGACCTCGTTCCAGCAAGCGCCGTTCGACGCATTTACCGACCCGGCCAAGGAAAAAACCGCACTCGGCGCCGATCT
GCTGAACGTGCGTGAAATCACGCTGATTCCGGGCCAGCGCTATACGGCAACCGAGAAGGTATCGCGCGAAGCGCAGGCAT
TCGGCATTGTCGCGCTGTTCCGCGATCCCGCACTGCAACGCTGGAAACTGACGTTCGATCCGGTGAAGTCGGAGAAATCC
GGCATAATCATCGGCCTGCATAATTGCGCGATGACGGTCACCGACGGCACGGTCATCGCACCGCAACAGGGTGCGCCTTC
GCAACCGCTGAATATGCTGTCTTCGGTCTCCTGCGGTTAA