Detailed information of component protein
Summary
Experimental investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | QU43_RS38220 |
| Coordinate (Strand) | 363015..363797 (-) |
| NCBI ID | WP_006491869.1 |
| RefSeq | NC_011000 |
| Uniprot ID | B4E6Q9_BURCJ, A0A364GSZ5_9BURK, A0A1D2PJ49_9BURK |
| KEGG ID | bcj:BCAL0337 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DotU | PF09850.11 | 8.6e-58 | 31..229 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-208 | MSYAPSLFGDNAPAPLHTPASTDSAFQARSLLDLLYDGFFMLFLLKNGREPDSASEFSTRIQEFLSDFERGAKKLNIAAEDVYGAKFAFCAAIDEMVLSSQFKIRADWERRPLQLVLFGEQLAGEKFFQYLEEGRAQGAARLQSLEVFHMCLLLGFQGKYLLEGPEKLAYLTARLGDEIAHMKGKRAPFAPHWPLPDQIAHRLKREVP |
| Transmembrane helix | 209-231 | VWAIGTVFALVALLGYLGLNTYL |
| Inside | 232-260 | KDKTLQALAPYSQVIKVGPESANLTISLP |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9848 | - | - |
Protein sequence: 261 a.a.
>T6CP002953 NC_011000:c363797-363015 [Burkholderia cenocepacia J2315] [TssL]
MSYAPSLFGDNAPAPLHTPASTDSAFQARSLLDLLYDGFFMLFLLKNGREPDSASEFSTRIQEFLSDFERGAKKLNIAAE
DVYGAKFAFCAAIDEMVLSSQFKIRADWERRPLQLVLFGEQLAGEKFFQYLEEGRAQGAARLQSLEVFHMCLLLGFQGKY
LLEGPEKLAYLTARLGDEIAHMKGKRAPFAPHWPLPDQIAHRLKREVPVWAIGTVFALVALLGYLGLNTYLKDKTLQALA
PYSQVIKVGPESANLTISLP*
MSYAPSLFGDNAPAPLHTPASTDSAFQARSLLDLLYDGFFMLFLLKNGREPDSASEFSTRIQEFLSDFERGAKKLNIAAE
DVYGAKFAFCAAIDEMVLSSQFKIRADWERRPLQLVLFGEQLAGEKFFQYLEEGRAQGAARLQSLEVFHMCLLLGFQGKY
LLEGPEKLAYLTARLGDEIAHMKGKRAPFAPHWPLPDQIAHRLKREVPVWAIGTVFALVALLGYLGLNTYLKDKTLQALA
PYSQVIKVGPESANLTISLP*
Nucleotide sequence: 783 bp
>T6CP002953 NC_011000:c363797-363015 [Burkholderia cenocepacia J2315] [TssL]
ATGAGCTACGCGCCTTCCTTGTTCGGCGACAACGCGCCGGCACCGCTGCATACCCCGGCGTCGACGGACTCCGCGTTCCA
GGCCCGTTCGCTGCTCGATTTGCTGTACGACGGGTTCTTCATGCTGTTCCTGCTCAAGAACGGACGGGAGCCGGACAGCG
CCAGCGAGTTCAGCACGCGGATCCAGGAATTCCTGTCGGATTTCGAGCGCGGCGCGAAGAAGCTGAATATCGCCGCCGAG
GACGTGTACGGCGCGAAGTTCGCGTTCTGCGCGGCCATCGACGAGATGGTGCTGTCGTCGCAGTTCAAGATCCGCGCGGA
CTGGGAACGCCGGCCGCTGCAGCTCGTGTTGTTCGGCGAGCAGCTCGCCGGCGAGAAGTTCTTCCAGTATCTCGAGGAAG
GTCGTGCGCAGGGTGCGGCACGGCTGCAGTCGCTCGAGGTGTTCCATATGTGCCTGCTGCTCGGGTTCCAGGGCAAGTAC
CTGCTCGAAGGGCCGGAAAAGCTCGCGTACCTGACCGCGCGGCTGGGCGACGAGATCGCGCACATGAAGGGCAAGCGCGC
GCCGTTCGCGCCGCACTGGCCGCTGCCGGACCAGATCGCGCACCGCTTGAAGCGCGAGGTGCCGGTGTGGGCGATCGGGA
CCGTGTTCGCGCTGGTGGCGCTGCTCGGGTACCTCGGGCTCAACACGTACCTGAAGGACAAGACGCTGCAAGCGCTCGCG
CCGTATTCGCAGGTGATCAAGGTCGGGCCGGAGTCGGCCAACCTGACGATCTCGTTGCCGTAA
ATGAGCTACGCGCCTTCCTTGTTCGGCGACAACGCGCCGGCACCGCTGCATACCCCGGCGTCGACGGACTCCGCGTTCCA
GGCCCGTTCGCTGCTCGATTTGCTGTACGACGGGTTCTTCATGCTGTTCCTGCTCAAGAACGGACGGGAGCCGGACAGCG
CCAGCGAGTTCAGCACGCGGATCCAGGAATTCCTGTCGGATTTCGAGCGCGGCGCGAAGAAGCTGAATATCGCCGCCGAG
GACGTGTACGGCGCGAAGTTCGCGTTCTGCGCGGCCATCGACGAGATGGTGCTGTCGTCGCAGTTCAAGATCCGCGCGGA
CTGGGAACGCCGGCCGCTGCAGCTCGTGTTGTTCGGCGAGCAGCTCGCCGGCGAGAAGTTCTTCCAGTATCTCGAGGAAG
GTCGTGCGCAGGGTGCGGCACGGCTGCAGTCGCTCGAGGTGTTCCATATGTGCCTGCTGCTCGGGTTCCAGGGCAAGTAC
CTGCTCGAAGGGCCGGAAAAGCTCGCGTACCTGACCGCGCGGCTGGGCGACGAGATCGCGCACATGAAGGGCAAGCGCGC
GCCGTTCGCGCCGCACTGGCCGCTGCCGGACCAGATCGCGCACCGCTTGAAGCGCGAGGTGCCGGTGTGGGCGATCGGGA
CCGTGTTCGCGCTGGTGGCGCTGCTCGGGTACCTCGGGCTCAACACGTACCTGAAGGACAAGACGCTGCAAGCGCTCGCG
CCGTATTCGCAGGTGATCAAGGTCGGGCCGGAGTCGGCCAACCTGACGATCTCGTTGCCGTAA