Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   ABFB52_RS24900 Genome accession   NZ_CP155585
Coordinates   5525259..5525681 (-) Length   140 a.a.
NCBI ID   WP_085747737.1    Uniprot ID   -
Organism   Pseudomonas sp. 9.2(2024)     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5520259..5530681
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFB52_RS24885 (ABFB52_24885) - 5521190..5524141 (+) 2952 WP_129972819.1 DUF748 domain-containing protein -
  ABFB52_RS24890 (ABFB52_24890) - 5524141..5524437 (+) 297 WP_085747738.1 DUF2845 domain-containing protein -
  ABFB52_RS24895 (ABFB52_24895) - 5524605..5524961 (-) 357 WP_011335977.1 BON domain-containing protein -
  ABFB52_RS24900 (ABFB52_24900) pilA/pilAI 5525259..5525681 (-) 423 WP_085747737.1 pilin Machinery gene
  ABFB52_RS24905 (ABFB52_24905) pilB 5525908..5527608 (+) 1701 WP_085747736.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFB52_RS24910 (ABFB52_24910) pilC 5527611..5528828 (+) 1218 WP_129972818.1 type II secretion system F family protein Machinery gene
  ABFB52_RS24915 (ABFB52_24915) pilD 5528830..5529702 (+) 873 WP_102686305.1 A24 family peptidase Machinery gene
  ABFB52_RS24920 (ABFB52_24920) coaE 5529699..5530322 (+) 624 WP_007959586.1 dephospho-CoA kinase -
  ABFB52_RS24925 (ABFB52_24925) yacG 5530319..5530519 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 14089.24 Da        Isoelectric Point: 8.4918

>NTDB_id=997963 ABFB52_RS24900 WP_085747737.1 5525259..5525681(-) (pilA/pilAI) [Pseudomonas sp. 9.2(2024)]
MKKQQGFTLIELLIVVAIIGILATVALPQYSKYQARSKVTAGLAEISAMKVPFEDIINQGTNPTLAAVKGDAGTTTSNCA
LTASGTAADGAGTIVCTLANAPAPVVGKTITLTRTAANGWSCATTVAPDYSPKGCTASGT

Nucleotide


Download         Length: 423 bp        

>NTDB_id=997963 ABFB52_RS24900 WP_085747737.1 5525259..5525681(-) (pilA/pilAI) [Pseudomonas sp. 9.2(2024)]
ATGAAGAAACAACAAGGTTTCACTCTGATCGAGCTGCTGATCGTTGTGGCGATCATCGGTATTCTCGCGACTGTGGCGCT
GCCGCAGTACTCCAAATATCAGGCGCGGTCGAAGGTGACGGCGGGGCTGGCCGAAATCTCGGCGATGAAAGTGCCGTTCG
AAGACATCATCAATCAAGGGACGAACCCCACGCTGGCTGCCGTTAAAGGCGATGCGGGCACTACCACCTCCAACTGTGCA
CTGACCGCCTCGGGGACAGCTGCAGATGGAGCGGGCACCATCGTTTGTACGCTGGCCAATGCGCCGGCACCGGTGGTGGG
CAAAACCATCACGCTCACACGAACCGCTGCCAACGGCTGGTCGTGTGCAACGACCGTTGCGCCGGACTACTCCCCTAAAG
GCTGTACAGCAAGCGGTACCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-136)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

51.095

97.857

0.5
  pilA Acinetobacter baumannii strain A118

46.763

99.286

0.464
  pilA Vibrio cholerae strain A1552

41.935

100

0.464
  pilA Vibrio cholerae C6706

41.935

100

0.464
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

41.935

100

0.464
  comP Acinetobacter baylyi ADP1

42.105

100

0.457
  pilA Pseudomonas aeruginosa PAK

39.61

100

0.436
  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.704

96.429

0.421
  pilA2 Legionella pneumophila str. Paris

42.143

100

0.421
  pilA2 Legionella pneumophila strain ERS1305867

41.429

100

0.414
  pilA Vibrio parahaemolyticus RIMD 2210633

42.963

96.429

0.414
  pilA/pilA1 Eikenella corrodens VA1

35.172

100

0.364

Multiple sequence alignment