Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ABFB52_RS24905 Genome accession   NZ_CP155585
Coordinates   5525908..5527608 (+) Length   566 a.a.
NCBI ID   WP_085747736.1    Uniprot ID   -
Organism   Pseudomonas sp. 9.2(2024)     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5520908..5532608
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFB52_RS24885 (ABFB52_24885) - 5521190..5524141 (+) 2952 WP_129972819.1 DUF748 domain-containing protein -
  ABFB52_RS24890 (ABFB52_24890) - 5524141..5524437 (+) 297 WP_085747738.1 DUF2845 domain-containing protein -
  ABFB52_RS24895 (ABFB52_24895) - 5524605..5524961 (-) 357 WP_011335977.1 BON domain-containing protein -
  ABFB52_RS24900 (ABFB52_24900) pilA/pilAI 5525259..5525681 (-) 423 WP_085747737.1 pilin Machinery gene
  ABFB52_RS24905 (ABFB52_24905) pilB 5525908..5527608 (+) 1701 WP_085747736.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ABFB52_RS24910 (ABFB52_24910) pilC 5527611..5528828 (+) 1218 WP_129972818.1 type II secretion system F family protein Machinery gene
  ABFB52_RS24915 (ABFB52_24915) pilD 5528830..5529702 (+) 873 WP_102686305.1 A24 family peptidase Machinery gene
  ABFB52_RS24920 (ABFB52_24920) coaE 5529699..5530322 (+) 624 WP_007959586.1 dephospho-CoA kinase -
  ABFB52_RS24925 (ABFB52_24925) yacG 5530319..5530519 (+) 201 WP_011335983.1 DNA gyrase inhibitor YacG -
  ABFB52_RS24930 (ABFB52_24930) - 5530526..5530741 (-) 216 WP_129972817.1 hypothetical protein -
  ABFB52_RS24935 (ABFB52_24935) - 5530807..5531496 (-) 690 WP_129972816.1 energy-coupling factor ABC transporter permease -
  ABFB52_RS24940 (ABFB52_24940) - 5531493..5531960 (-) 468 WP_085689765.1 FAD/FMN-containing dehydrogenase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62818.81 Da        Isoelectric Point: 5.9468

>NTDB_id=997964 ABFB52_RS24905 WP_085747736.1 5525908..5527608(+) (pilB) [Pseudomonas sp. 9.2(2024)]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHATGLEEMADVDLDG
LDIESADDSRQDTLGGIDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVMRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPAGCDHCNGGYKGRVGIYEVVKNTPELQRLIMAEGNSLEIDTQMRRDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=997964 ABFB52_RS24905 WP_085747736.1 5525908..5527608(+) (pilB) [Pseudomonas sp. 9.2(2024)]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAACAACTGGTCCAGGCCGAATTGCTCACGGAAAAGAGTGCCCAGCAGGC
CTGGCAGCAGGCGCAGCGCAATCGCCTGTCGCTGGTCAGTTACCTGGTGCAGAACAAACTGGTGAAGAGCTGGCAGGTCG
CCGAAATAGCCTCGGAGCATTTCGGCATGGCATTTCTCGACCTCAACTGTCTCGACAAGGAAACCCAGCCCAAGGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCACCACGCCCTGCCCCTCTGGCGGCGCGGCAACAAACTGTTCGTGGGGATTTCCGA
CCCGAGCAATCACCAGGCGATCAATGACATTCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAAGACGACA
AACTCACCGACGCCATTGAAAAATTCTTCGACACCCACGCCACCGGCCTGGAGGAGATGGCCGATGTCGACCTCGACGGG
CTGGACATCGAGTCGGCCGACGACAGCAGACAGGACACCCTCGGCGGAATCGACGCCGACGACGCTCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTGGACGCGATCAAGAGCGGCTCGTCCGACCTGCACTTCGAGCCTTACGAAAAGAACTACCGGG
TGCGGGTGCGCACCGACGGCATTCTGCGGGAGGTGGCCAAGCCGCCGATTCAGCTCGCCGGGCGCATTGCCGCGCGCCTG
AAGGTCATGGCCAGCCTCGACATTTCAGAACGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGTCTGTCGAAGAGCAA
GTCGATCGATTTTCGGGTCAACACCCTGCCCACGCTCTGGGGCGAGAAAGTGGTGATGCGGATCCTCGACCCCTCCAGCG
CCCAGATCGGCATCGATGCCCTCGGCTACGAGCCGGAACAGAAAGACCTGTACATGGCCGCCCTCAAGCAGCCCCAAGGC
ATGATTCTGGTAACCGGCCCCACGGGGTCCGGCAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAATACCGTCGA
CATCAACATCTCCACCGCCGAAGATCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCACGCCAAG
GGTTGGACTTTGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTC
GAAACCGCCGAAATCGCGATCAAGGCCGCCCAGACCGGTCACCTCGTGCTGTCCACCCTGCACACCAACAGCGCAGCAGA
GACCCTCACCCGCCTGCACAACATGGGCATCCCCGGCTTCAACATCGCGACCTCGGTGAGCCTGATCATTGCCCAGCGTC
TGGCGCGAAAACTCTGCAGCCACTGCAAGAAACCCATCGAGATCCCCCGCGAAACCCTGATCAAGGAAGGTTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTATGAGCCCGCCGGTTGCGATCACTGCAACGGCGGGTACAAGGGTCGCGTGGGGAT
TTATGAAGTGGTGAAGAACACCCCCGAACTGCAACGGCTGATCATGGCCGAGGGCAACTCACTGGAAATCGATACCCAGA
TGCGCCGGGACGGCTTCAACGACCTGCGCACCTCGGGCCTGATCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.053

99.823

0.58
  pilB Acinetobacter baumannii D1279779

57.979

99.647

0.578
  pilB Legionella pneumophila strain ERS1305867

56.537

100

0.565
  pilB Vibrio cholerae strain A1552

51.146

100

0.512
  pilB Vibrio campbellii strain DS40M4

48.438

100

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

49.554

99.117

0.491
  pilF Neisseria gonorrhoeae MS11

49.201

99.47

0.489
  pilF Thermus thermophilus HB27

37.097

98.587

0.366
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.826

93.286

0.362

Multiple sequence alignment