Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   QUD34_RS11215 Genome accession   NZ_AP027079
Coordinates   2436714..2437871 (-) Length   385 a.a.
NCBI ID   WP_375380022.1    Uniprot ID   -
Organism   Geothrix oryzae strain Red222     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2431714..2442871
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUD34_RS11190 (GETHOR_21690) - 2431886..2432425 (+) 540 WP_286353787.1 type II secretion system protein -
  QUD34_RS11195 (GETHOR_21700) - 2432530..2433036 (+) 507 WP_286353788.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QUD34_RS11200 (GETHOR_21710) - 2432990..2434879 (-) 1890 WP_286353789.1 O-antigen ligase family protein -
  QUD34_RS11205 (GETHOR_21720) - 2434964..2435395 (-) 432 WP_286353790.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  QUD34_RS11210 (GETHOR_21730) - 2435441..2436643 (-) 1203 WP_286353791.1 type II secretion system F family protein -
  QUD34_RS11215 (GETHOR_21740) pilT 2436714..2437871 (-) 1158 WP_375380022.1 type IV pilus twitching motility protein PilT Machinery gene
  QUD34_RS11220 (GETHOR_21750) pilB 2437907..2439745 (-) 1839 WP_286353793.1 type IV-A pilus assembly ATPase PilB -
  QUD34_RS11225 (GETHOR_21760) - 2439825..2441783 (+) 1959 WP_286353794.1 hypothetical protein -

Sequence

Protein


Download         Length: 385 a.a.        Molecular weight: 42308.38 Da        Isoelectric Point: 7.5663

>NTDB_id=99474 QUD34_RS11215 WP_375380022.1 2436714..2437871(-) (pilT) [Geothrix oryzae strain Red222]
MAPLQQLLKTMVEYGGTDLHITTDSAPQIRIDGRMVPLKLPPLDASQTRWLCYGVMTDQQKHRLEEDLEVDFSFGLQGVA
RFRANVFNQRGATAGVFRTIPENIRSFDQLGLPPSVQALCDKPRGLVLVTGVTGSGKSTTLAAMVDKINAEEPVHILTIE
DPVEYVHKHRRALVNQREIHADTHSFKKALRSALRQDPDVVLVGELRDLETIESALTIAETGHLTFATLHTNSTVQTINR
IIDVFPSHQQPQVRAQLSLVLEGVICQSLIPKAGGKGRALALEIMIPNAAIRNLIREDKIHQIYGTMQAGQTKYGMQTFN
QSLSDLVLKKEITQEAAFDYSSNTDELRELINRGVGVGTAGGRTATPASPVNPALGGRNPNIKYT

Nucleotide


Download         Length: 1158 bp        

>NTDB_id=99474 QUD34_RS11215 WP_375380022.1 2436714..2437871(-) (pilT) [Geothrix oryzae strain Red222]
GTGGCCCCGTTGCAGCAGCTCCTCAAGACCATGGTGGAGTACGGGGGAACGGACCTCCACATCACCACGGATTCCGCGCC
CCAGATCCGCATCGACGGGCGCATGGTCCCCCTCAAGCTGCCGCCCCTGGACGCTTCCCAGACCCGTTGGCTCTGCTACG
GGGTCATGACCGACCAGCAGAAGCATCGCCTGGAGGAGGATCTGGAGGTGGACTTCTCGTTCGGCCTCCAGGGCGTGGCC
CGGTTCCGCGCCAATGTCTTCAACCAGCGCGGCGCCACGGCGGGCGTGTTCCGCACCATCCCCGAGAACATCCGCAGCTT
CGATCAGCTGGGCTTACCACCCTCGGTGCAGGCCCTCTGCGACAAGCCCCGGGGCCTGGTTCTGGTCACGGGTGTGACCG
GATCCGGCAAGTCCACGACTCTGGCCGCCATGGTGGACAAGATCAACGCCGAGGAGCCGGTCCACATCCTCACCATCGAG
GACCCGGTGGAATATGTCCACAAGCACCGCCGGGCCCTGGTGAACCAGCGCGAGATCCATGCGGACACCCACAGCTTCAA
GAAAGCGCTGCGATCAGCCCTCCGCCAGGATCCCGATGTGGTGCTGGTGGGCGAGCTGCGGGATCTGGAGACCATCGAGT
CGGCCCTCACCATCGCCGAGACGGGCCACCTCACCTTCGCCACCCTGCATACCAACAGCACGGTGCAGACCATCAACCGC
ATCATCGATGTCTTCCCGTCCCACCAGCAGCCGCAGGTGCGGGCCCAGCTTTCGCTGGTGCTGGAAGGCGTCATTTGTCA
GAGCCTCATTCCCAAGGCCGGCGGCAAGGGGCGCGCCCTGGCATTGGAGATCATGATCCCCAACGCCGCCATCCGGAACC
TCATCCGCGAGGACAAGATCCACCAGATCTACGGCACCATGCAGGCCGGCCAGACGAAGTACGGCATGCAGACCTTCAAC
CAGAGCCTGTCCGACCTGGTGCTGAAGAAGGAGATCACGCAGGAAGCCGCCTTCGACTACTCCTCCAACACCGACGAACT
GCGCGAGCTCATCAACCGGGGCGTGGGCGTCGGCACCGCCGGCGGCCGGACCGCCACCCCGGCCTCGCCCGTCAATCCGG
CCCTTGGGGGGCGGAACCCCAACATCAAGTACACCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Legionella pneumophila strain Lp02

54.412

88.312

0.481
  pilT Legionella pneumophila strain ERS1305867

54.412

88.312

0.481
  pilT Pseudomonas aeruginosa PAK

53.593

86.753

0.465
  pilT Acinetobacter baumannii D1279779

52.994

86.753

0.46
  pilT Acinetobacter baumannii strain A118

52.994

86.753

0.46
  pilT Acinetobacter nosocomialis M2

52.994

86.753

0.46
  pilT Acinetobacter baylyi ADP1

53.988

84.675

0.457
  pilT Pseudomonas stutzeri DSM 10701

52.395

86.753

0.455
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

46.9

96.364

0.452
  pilT Neisseria gonorrhoeae MS11

50.147

88.571

0.444
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.454

84.675

0.444
  pilT Vibrio cholerae strain A1552

52.454

84.675

0.444
  pilT Neisseria meningitidis 8013

50.904

86.234

0.439
  pilU Pseudomonas stutzeri DSM 10701

41.88

91.169

0.382
  pilU Vibrio cholerae strain A1552

39.665

92.987

0.369

Multiple sequence alignment