Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAHF05_RS18695 Genome accession   NZ_CP154353
Coordinates   3851337..3852335 (-) Length   332 a.a.
NCBI ID   WP_129323754.1    Uniprot ID   -
Organism   Enterobacter adelaidei strain ECC3473     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3846337..3857335
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHF05_RS18665 (AAHF05_18640) - 3847229..3847726 (+) 498 WP_129323751.1 SprT family zinc-dependent metalloprotease -
  AAHF05_RS18670 (AAHF05_18645) endA 3847821..3848528 (+) 708 WP_028018378.1 deoxyribonuclease I -
  AAHF05_RS18675 (AAHF05_18650) rsmE 3848581..3849312 (+) 732 WP_129323752.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AAHF05_RS18680 (AAHF05_18655) gshB 3849332..3850279 (+) 948 WP_129323753.1 glutathione synthase -
  AAHF05_RS18685 (AAHF05_18660) - 3850367..3850927 (+) 561 WP_028018381.1 YqgE/AlgH family protein -
  AAHF05_RS18690 (AAHF05_18665) ruvX 3850927..3851343 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  AAHF05_RS18695 (AAHF05_18670) pilT 3851337..3852335 (-) 999 WP_129323754.1 type IV pilus twitching motility protein PilT Machinery gene
  AAHF05_RS18700 (AAHF05_18675) - 3852353..3853054 (+) 702 WP_129323755.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAHF05_RS18705 (AAHF05_18680) - 3853076..3853642 (+) 567 WP_028018384.1 YggT family protein -
  AAHF05_RS18710 (AAHF05_18685) yggU 3853639..3853935 (+) 297 WP_129323756.1 DUF167 family protein YggU -
  AAHF05_RS18715 (AAHF05_18690) - 3853939..3854532 (+) 594 WP_129323757.1 XTP/dITP diphosphatase -
  AAHF05_RS18720 (AAHF05_18695) hemW 3854525..3855667 (+) 1143 WP_128347278.1 radical SAM family heme chaperone HemW -
  AAHF05_RS18725 (AAHF05_18700) - 3855725..3855973 (+) 249 WP_332614626.1 hypothetical protein -
  AAHF05_RS18730 (AAHF05_18705) - 3855859..3856575 (-) 717 WP_008499762.1 DUF2884 domain-containing protein -
  AAHF05_RS18735 (AAHF05_18710) - 3856632..3856958 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 332 a.a.        Molecular weight: 36326.46 Da        Isoelectric Point: 6.2454

>NTDB_id=993044 AAHF05_RS18695 WP_129323754.1 3851337..3852335(-) (pilT) [Enterobacter adelaidei strain ECC3473]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVESLLKAWLNDEQQGAWWANGQVDFAVTIAGGQRLRG
SAFKQMKGVSVTLRLLPRSCPQLFSLGVPRAVPELLTNDNGLILVTGATGSGKSTTLAAMVDFLNRNTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDIILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQTGMQNFDQSLAER
RAQGRLQAPCAQ

Nucleotide


Download         Length: 999 bp        

>NTDB_id=993044 AAHF05_RS18695 WP_129323754.1 3851337..3852335(-) (pilT) [Enterobacter adelaidei strain ECC3473]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCCCG
ATGGCGCAGATTAGGCCGTCTTGAACCGGCACCGTTTCCGCCCCCTGATGTCGAGTCACTGCTAAAAGCGTGGCTCAACG
ATGAACAGCAAGGAGCATGGTGGGCAAACGGACAAGTGGATTTTGCCGTAACCATTGCAGGAGGCCAGCGTTTGCGCGGC
AGTGCCTTTAAACAGATGAAGGGGGTCTCTGTGACGCTACGGCTGTTGCCGCGAAGCTGTCCGCAACTCTTTTCGCTGGG
GGTACCACGGGCTGTGCCGGAACTGCTAACGAACGACAACGGATTGATTCTGGTCACGGGGGCGACCGGCAGCGGCAAAT
CCACCACGCTGGCGGCGATGGTCGATTTTCTCAACCGCAATACTGACGGCCATATCCTGACGCTCGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGCTGCCTGATACAGCAGCGAGAGATTGGCCAGCACAGTCCGTCATTCGCCGAGGCGCTACG
TAGCGCGTTACGCGAGGATCCTGACATTATTCTGCTGGGGGAACTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCTGAAACCGGGCATCTGGTGCTGGCAACATTGCATACGCGCGGTGCATCCCAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCTCAGGAAAAAGATCCGGTGCGTAATCAACTGGCCGGCAGCCTGCGGGCGGTACTGGCGCAAAAGCTACTCCC
TGATTTACAGGGTGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCGCTGCCGCGGCGAATCTCATTCGTGAAGGAA
AAACATGGCAACTGCCCGGCATCATTCAAACCGGTCAACAAACGGGGATGCAGAACTTTGACCAGAGCCTGGCAGAGAGG
CGGGCACAGGGGCGACTTCAGGCTCCCTGTGCTCAGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.955

100

0.494
  pilT Vibrio cholerae strain A1552

48.955

100

0.494
  pilT Legionella pneumophila strain Lp02

50

96.988

0.485
  pilT Legionella pneumophila strain ERS1305867

50

96.988

0.485
  pilT Pseudomonas stutzeri DSM 10701

47.463

100

0.479
  pilT Acinetobacter baumannii D1279779

47.463

100

0.479
  pilT Acinetobacter baumannii strain A118

47.463

100

0.479
  pilT Acinetobacter baylyi ADP1

47.164

100

0.476
  pilT Neisseria gonorrhoeae MS11

46.866

100

0.473
  pilT Neisseria meningitidis 8013

47.147

100

0.473
  pilT Acinetobacter nosocomialis M2

48.75

96.386

0.47
  pilT Pseudomonas aeruginosa PAK

47.401

98.494

0.467
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.168

96.988

0.419
  pilU Pseudomonas stutzeri DSM 10701

39.091

99.398

0.389
  pilU Vibrio cholerae strain A1552

38.82

96.988

0.377

Multiple sequence alignment