Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   AAHB66_RS19475 Genome accession   NZ_CP152474
Coordinates   4075948..4076925 (-) Length   325 a.a.
NCBI ID   WP_347114140.1    Uniprot ID   -
Organism   Leclercia sp. S52     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4070948..4081925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAHB66_RS19445 (AAHB66_19450) endA 4071533..4072240 (+) 708 WP_333853927.1 deoxyribonuclease I -
  AAHB66_RS19450 (AAHB66_19455) rsmE 4072354..4073085 (+) 732 WP_347114138.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  AAHB66_RS19455 (AAHB66_19460) gshB 4073105..4074052 (+) 948 WP_333853932.1 glutathione synthase -
  AAHB66_RS19460 (AAHB66_19465) - 4074144..4074704 (+) 561 WP_142487043.1 YqgE/AlgH family protein -
  AAHB66_RS19465 (AAHB66_19470) ruvX 4074704..4075120 (+) 417 WP_180209603.1 Holliday junction resolvase RuvX -
  AAHB66_RS19470 (AAHB66_19475) - 4075159..4075848 (-) 690 WP_347114139.1 IclR family transcriptional regulator -
  AAHB66_RS19475 (AAHB66_19480) pilT 4075948..4076925 (-) 978 WP_347114140.1 type IV pilus twitching motility protein PilT Machinery gene
  AAHB66_RS19480 (AAHB66_19485) - 4076943..4077644 (+) 702 WP_347114141.1 YggS family pyridoxal phosphate-dependent enzyme -
  AAHB66_RS19485 (AAHB66_19490) - 4077666..4078232 (+) 567 WP_032613937.1 YggT family protein -
  AAHB66_RS19490 (AAHB66_19495) yggU 4078229..4078525 (+) 297 WP_333853941.1 DUF167 family protein YggU -
  AAHB66_RS19495 (AAHB66_19500) - 4078529..4079122 (+) 594 WP_347114143.1 XTP/dITP diphosphatase -
  AAHB66_RS19500 (AAHB66_19505) hemW 4079115..4080257 (+) 1143 WP_347114144.1 radical SAM family heme chaperone HemW -
  AAHB66_RS19505 (AAHB66_19510) - 4080321..4080659 (+) 339 WP_347114145.1 endonuclease domain-containing protein -
  AAHB66_RS19510 (AAHB66_19515) - 4080780..4081499 (-) 720 WP_347114146.1 DUF2884 domain-containing protein -
  AAHB66_RS19515 (AAHB66_19520) - 4081552..4081878 (-) 327 WP_142487055.1 YggL family protein -

Sequence

Protein


Download         Length: 325 a.a.        Molecular weight: 35606.86 Da        Isoelectric Point: 7.2150

>NTDB_id=989602 AAHB66_RS19475 WP_347114140.1 4075948..4076925(-) (pilT) [Leclercia sp. S52]
MDMEEIVALSVKHNVSDLHLCSNAPPRWRRRGKIEFAPFPGPDVVKLLKSWLSDEQQGAWCAQGQVDFAVSLEALRLRGS
AFAHTQGCSLALRLLPERCPQLRTLGTPRAIPELLTSDNGLILVTGATGSGKSTTLAAMVDFLNHHSDGHILTLEDPVEF
IHQSKRCLVQQREIGLHCPSFADALRAALREDPDVLLLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDAF
PAEEKEPVRKQLAGSLRAVLAQKLERDNQQGRVALFELLVNTPAAANLIREGKTFQLPGVIQTGLQAGMLNFDQSRAERR
AQGRL

Nucleotide


Download         Length: 978 bp        

>NTDB_id=989602 AAHB66_RS19475 WP_347114140.1 4075948..4076925(-) (pilT) [Leclercia sp. S52]
ATGGATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTAACGCACCACCGCG
CTGGCGGCGACGAGGGAAGATAGAATTCGCCCCGTTTCCCGGGCCGGATGTGGTGAAGCTGCTCAAAAGCTGGCTCAGCG
ACGAACAGCAGGGAGCATGGTGCGCCCAGGGGCAGGTGGATTTTGCCGTCAGCCTGGAGGCACTCCGGCTGCGGGGCAGC
GCCTTCGCCCATACGCAAGGGTGCTCGCTGGCGCTCCGGCTGCTGCCGGAACGGTGCCCGCAGCTGCGCACCCTGGGCAC
GCCGCGGGCGATCCCCGAGCTGCTGACCAGCGATAACGGCCTGATCCTGGTGACCGGGGCCACCGGCAGCGGCAAGTCCA
CCACCCTGGCGGCGATGGTCGACTTTCTCAATCACCACAGCGACGGGCATATTCTGACCCTGGAAGATCCGGTTGAGTTT
ATCCATCAGAGCAAGCGTTGTCTGGTGCAGCAGCGGGAGATTGGCCTGCATTGTCCCTCTTTTGCCGATGCGCTGCGGGC
GGCGCTGCGTGAAGATCCCGACGTGCTTCTGTTAGGCGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTCACCGCCG
CAGAGACCGGACATCTGGTGCTGGCGACCCTGCATACCCGGGGTGCCGCCCAGGCGATAGAGCGGCTGGTGGATGCCTTC
CCGGCCGAGGAGAAAGAGCCGGTACGTAAGCAGCTGGCCGGCAGCCTGCGCGCGGTGCTGGCGCAAAAGCTCGAAAGGGA
TAACCAGCAGGGACGCGTCGCGCTGTTTGAGCTGCTGGTTAATACCCCTGCGGCGGCCAATTTGATCCGTGAAGGCAAAA
CCTTTCAACTGCCCGGGGTGATCCAGACCGGCCTGCAGGCGGGAATGCTAAACTTCGATCAGAGCCGGGCTGAGCGCAGG
GCGCAGGGGCGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(64-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.624

100

0.489
  pilT Vibrio cholerae strain A1552

48.624

100

0.489
  pilT Legionella pneumophila strain Lp02

48.447

99.077

0.48
  pilT Legionella pneumophila strain ERS1305867

48.447

99.077

0.48
  pilT Acinetobacter baylyi ADP1

46.483

100

0.468
  pilT Neisseria meningitidis 8013

47.188

98.462

0.465
  pilT Pseudomonas stutzeri DSM 10701

47.634

97.538

0.465
  pilT Acinetobacter baumannii D1279779

47.022

98.154

0.462
  pilT Acinetobacter baumannii strain A118

47.022

98.154

0.462
  pilT Acinetobacter nosocomialis M2

47.022

98.154

0.462
  pilT Neisseria gonorrhoeae MS11

46.875

98.462

0.462
  pilT Pseudomonas aeruginosa PAK

47.003

97.538

0.458
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

44.34

97.846

0.434
  pilU Vibrio cholerae strain A1552

40.252

97.846

0.394
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.388

Multiple sequence alignment