Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA/cglA/cilD   Type   Machinery gene
Locus tag   R8586_RS10360 Genome accession   NZ_AP026922
Coordinates   1964659..1965600 (-) Length   313 a.a.
NCBI ID   WP_000249571.1    Uniprot ID   A0A0B7M3Q7
Organism   Streptococcus pneumoniae strain PZ900700038     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1959659..1970600
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8586_RS10320 - 1959809..1960610 (+) 802 WP_128887635.1 IS5 family transposase -
  R8586_RS10325 (PC0038_20290) - 1960761..1961237 (+) 477 WP_000777686.1 GNAT family N-acetyltransferase -
  R8586_RS10330 (PC0038_20300) comGG/cglG 1961911..1962303 (-) 393 WP_001818966.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8586_RS10335 (PC0038_20310) comGF/cglF 1962281..1962742 (-) 462 WP_000250537.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8586_RS10340 (PC0038_20320) comGE/cglE 1962705..1963007 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8586_RS10345 (PC0038_20330) comGD/cglD 1962970..1963374 (-) 405 WP_000588017.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8586_RS10350 (PC0038_20340) comGC/cglC 1963367..1963693 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  R8586_RS10355 (PC0038_20350) comGB/cglB 1963695..1964711 (-) 1017 WP_001844019.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8586_RS10360 (PC0038_20360) comGA/cglA/cilD 1964659..1965600 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  R8586_RS10365 (PC0038_20370) - 1965676..1966041 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8586_RS10370 (PC0038_20380) - 1966192..1967250 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  R8586_RS10375 (PC0038_20390) nagA 1967413..1968564 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -
  R8586_RS10380 (PC0038_20400) - 1968717..1970534 (-) 1818 WP_001220841.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 313 a.a.        Molecular weight: 35591.51 Da        Isoelectric Point: 6.0083

>NTDB_id=98146 R8586_RS10360 WP_000249571.1 1964659..1965600(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700038]
MVQEIAQEIIRSARKKGTQDIYFVPKLDAYELHMRVGDERCKIGSYDFEKFAAVISHFKFVAGMNVGEKRRSQLGSCDYA
YDQKMASLRLSTVGDYRGHESLVIRLLHDEEQDLHFWFQDIEELGKQYRQRGLYLFAGPVGSGKTTLMHELSKSLFKGQQ
VMSIEDPVEIKQDDMLQLQLNEAIGLTYENLIKLSLRHRPDLLIIGEIRDSETARAVVRASLTGATVFSTIHAKSIRGVY
ERLLELGVSEEELAVVLQGVCYQRLIGGGGIVDFANRDYQEHQAAKWNEQIDQLLKDGHITSLQAETEKISYS

Nucleotide


Download         Length: 942 bp        

>NTDB_id=98146 R8586_RS10360 WP_000249571.1 1964659..1965600(-) (comGA/cglA/cilD) [Streptococcus pneumoniae strain PZ900700038]
ATGGTTCAAGAAATTGCACAAGAAATCATTCGTTCAGCTCGGAAAAAAGGGACGCAGGATATTTATTTTGTCCCTAAGTT
AGACGCCTATGAGCTTCATATGAGGGTAGGAGACGAGCGCTGTAAAATTGGTAGCTATGATTTTGAAAAGTTTGCAGCCG
TTATCAGTCACTTTAAGTTTGTGGCGGGTATGAATGTGGGAGAAAAAAGACGTAGTCAACTGGGTTCCTGTGATTATGCC
TATGACCAGAAGATGGCGTCTCTACGTTTATCTACTGTAGGCGATTATCGGGGGCATGAGAGTTTGGTTATCCGTTTGTT
GCACGATGAGGAGCAGGACTTGCATTTTTGGTTTCAGGATATTGAAGAATTAGGCAAGCAGTACAGGCAACGGGGACTCT
ATCTTTTTGCTGGCCCGGTTGGGAGTGGTAAGACGACCTTGATGCATGAATTGTCCAAGTCACTCTTTAAAGGACAGCAA
GTTATGTCCATCGAAGATCCTGTCGAAATCAAGCAGGACGACATGCTTCAGTTGCAGTTGAACGAAGCAATCGGCCTAAC
CTATGAAAATCTAATCAAACTTTCCTTGCGTCATCGACCAGATCTCTTGATTATCGGAGAAATTCGTGACAGCGAGACGG
CGCGTGCAGTGGTCAGAGCTAGTTTGACAGGTGCGACAGTCTTTTCAACCATTCACGCCAAGAGTATCCGAGGTGTTTAT
GAGCGTCTGCTGGAGTTGGGTGTGAGTGAAGAAGAATTGGCAGTTGTTCTGCAAGGAGTCTGCTACCAGAGATTAATCGG
GGGAGGAGGAATCGTTGACTTTGCAAACAGAGATTATCAAGAACACCAAGCAGCCAAGTGGAATGAGCAAATTGACCAAC
TTCTTAAAGATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0B7M3Q7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae D39

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae R6

99.042

100

0.99
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

99.042

100

0.99
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

96.805

100

0.968
  comYA Streptococcus gordonii str. Challis substr. CH1

77.742

99.042

0.77
  comYA Streptococcus mutans UA159

65.916

99.361

0.655
  comYA Streptococcus mutans UA140

65.916

99.361

0.655
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

62.581

99.042

0.62
  comGA Lactococcus lactis subsp. cremoris KW2

54.808

99.681

0.546
  comGA Latilactobacillus sakei subsp. sakei 23K

42.642

84.665

0.361

Multiple sequence alignment