Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGB/cglB   Type   Machinery gene
Locus tag   R8586_RS10355 Genome accession   NZ_AP026922
Coordinates   1963695..1964711 (-) Length   338 a.a.
NCBI ID   WP_001844019.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain PZ900700038     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1958695..1969711
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  R8586_RS10320 - 1959809..1960610 (+) 802 WP_128887635.1 IS5 family transposase -
  R8586_RS10325 (PC0038_20290) - 1960761..1961237 (+) 477 WP_000777686.1 GNAT family N-acetyltransferase -
  R8586_RS10330 (PC0038_20300) comGG/cglG 1961911..1962303 (-) 393 WP_001818966.1 competence type IV pilus minor pilin ComGG Machinery gene
  R8586_RS10335 (PC0038_20310) comGF/cglF 1962281..1962742 (-) 462 WP_000250537.1 competence type IV pilus minor pilin ComGF Machinery gene
  R8586_RS10340 (PC0038_20320) comGE/cglE 1962705..1963007 (-) 303 WP_000413382.1 competence type IV pilus minor pilin ComGE Machinery gene
  R8586_RS10345 (PC0038_20330) comGD/cglD 1962970..1963374 (-) 405 WP_000588017.1 competence type IV pilus minor pilin ComGD Machinery gene
  R8586_RS10350 (PC0038_20340) comGC/cglC 1963367..1963693 (-) 327 WP_000738625.1 competence type IV pilus major pilin ComGC Machinery gene
  R8586_RS10355 (PC0038_20350) comGB/cglB 1963695..1964711 (-) 1017 WP_001844019.1 competence type IV pilus assembly protein ComGB Machinery gene
  R8586_RS10360 (PC0038_20360) comGA/cglA/cilD 1964659..1965600 (-) 942 WP_000249571.1 competence type IV pilus ATPase ComGA Machinery gene
  R8586_RS10365 (PC0038_20370) - 1965676..1966041 (-) 366 WP_000286415.1 DUF1033 family protein -
  R8586_RS10370 (PC0038_20380) - 1966192..1967250 (-) 1059 WP_000649471.1 zinc-dependent alcohol dehydrogenase family protein -
  R8586_RS10375 (PC0038_20390) nagA 1967413..1968564 (-) 1152 WP_001134457.1 N-acetylglucosamine-6-phosphate deacetylase -

Sequence

Protein


Download         Length: 338 a.a.        Molecular weight: 38540.51 Da        Isoelectric Point: 9.4800

>NTDB_id=98145 R8586_RS10355 WP_001844019.1 1963695..1964711(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900700038]
MDISQVFRLRRKKLATAKQKNIITLFNNLFSSGFHLVETISFLDRSSLLDKQCVTQMRTGLSQGKSFSEMMESLGCSSTI
VTQLSLAEVHGNLHLSLGKIEEYLDNLAKVKKKLIEVATYPLILLDFLLLIMLGLRNYLLPQLDSSNIATRIIGNLPQIF
LGMVGLVSVLALLALTFYKRSSKMSVFSILARLPFIGIFVQTYLTAYYAREWGNMISQGMELTQIFQMMQEQGSQLFKEI
GQDLAQTLKNGREFSQTIGTYPFFRKELSLIIEYGEVKSKLGSELEIYAEKTWEAFFTRVNRTMNLVQPLVFIFVALIIV
LLYAAMLMPMYQNMEVNF

Nucleotide


Download         Length: 1017 bp        

>NTDB_id=98145 R8586_RS10355 WP_001844019.1 1963695..1964711(-) (comGB/cglB) [Streptococcus pneumoniae strain PZ900700038]
ATGGACATATCACAAGTCTTCAGGCTGAGACGGAAAAAATTAGCTACAGCTAAGCAAAAAAATATCATCACCCTATTTAA
CAATCTCTTTTCTAGCGGTTTTCATCTGGTGGAGACTATCTCCTTTTTAGATAGGAGTTCCTTGTTGGACAAGCAGTGTG
TGACCCAGATGCGTACAGGCTTGTCTCAGGGGAAATCATTCTCAGAAATGATGGAAAGTTTGGGATGTTCAAGTACCATT
GTCACTCAGTTATCTCTAGCTGAAGTTCATGGCAATCTTCACCTGAGTTTGGGAAAGATAGAAGAATATCTAGACAATCT
GGCCAAGGTCAAGAAAAAGTTAATTGAAGTAGCGACCTATCCTTTGATTTTGCTGGATTTTCTTCTCTTAATTATGCTGG
GGCTACGGAATTACCTGCTCCCACAACTGGATAGTAGCAATATTGCCACCCGAATTATCGGTAATCTGCCACAAATTTTT
CTAGGCATGGTAGGGCTTGTTTCCGTGCTTGCCCTTTTAGCACTAACTTTTTATAAAAGAAGTTCTAAGATGAGTGTCTT
TTCTATCTTAGCACGCCTTCCCTTTATTGGAATCTTTGTGCAGACCTACTTGACAGCCTATTATGCACGTGAATGGGGGA
ATATGATTTCACAGGGAATGGAGCTGACGCAGATTTTTCAAATGATGCAGGAACAAGGTTCCCAGCTCTTTAAAGAAATC
GGTCAAGATCTGGCTCAAACCCTGAAAAATGGCCGTGAATTTTCTCAGACGATAGGAACCTATCCTTTCTTTAGGAAGGA
ATTGAGTCTCATCATAGAGTATGGGGAAGTTAAGTCCAAGCTGGGTAGTGAGTTGGAAATCTATGCTGAAAAAACTTGGG
AAGCCTTTTTTACCCGAGTCAACCGCACCATGAATTTGGTGCAGCCACTGGTTTTTATCTTTGTGGCACTGATTATCGTT
TTACTTTATGCGGCAATGCTCATGCCCATGTATCAAAATATGGAGGTAAATTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(24-141)

Type II secretion system (T2SS), protein F (T2SSF)

(209-329)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGB/cglB Streptococcus pneumoniae Rx1

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae D39

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae R6

98.225

100

0.982
  comGB/cglB Streptococcus pneumoniae TIGR4

98.225

100

0.982
  comGB/cglB Streptococcus mitis SK321

94.675

100

0.947
  comGB/cglB Streptococcus mitis NCTC 12261

93.787

100

0.938
  comYB Streptococcus gordonii str. Challis substr. CH1

70.238

99.408

0.698
  comYB Streptococcus mutans UA140

58.176

94.083

0.547
  comYB Streptococcus mutans UA159

58.176

94.083

0.547
  comGB Lactococcus lactis subsp. cremoris KW2

50.898

98.817

0.503

Multiple sequence alignment