Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WKK05_RS31960 Genome accession   NZ_CP151099
Coordinates   7384133..7385254 (-) Length   373 a.a.
NCBI ID   WP_341527010.1    Uniprot ID   -
Organism   Nostoc sp. UHCC 0302     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7379133..7390254
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKK05_RS31925 (WKK05_31490) - 7379488..7379811 (-) 324 WP_341527003.1 type II toxin-antitoxin system PemK/MazF family toxin -
  WKK05_RS31930 (WKK05_31495) - 7379805..7380065 (-) 261 WP_341527004.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
  WKK05_RS31935 (WKK05_31500) - 7380068..7380478 (-) 411 WP_341527005.1 type II toxin-antitoxin system VapC family toxin -
  WKK05_RS31940 (WKK05_31505) - 7380482..7380736 (-) 255 WP_341527006.1 hypothetical protein -
  WKK05_RS31945 (WKK05_31510) - 7381012..7382034 (+) 1023 WP_341527007.1 saccharopine dehydrogenase-like oxidoreductase -
  WKK05_RS31950 (WKK05_31515) - 7382100..7382660 (-) 561 WP_341527008.1 hypothetical protein -
  WKK05_RS31955 (WKK05_31520) - 7382834..7384057 (-) 1224 WP_341527009.1 type II secretion system F family protein -
  WKK05_RS31960 (WKK05_31525) pilT 7384133..7385254 (-) 1122 WP_341527010.1 type IV pilus twitching motility protein PilT Machinery gene
  WKK05_RS31965 (WKK05_31530) pilB/pilB1 7385643..7387655 (-) 2013 WP_341527011.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  WKK05_RS31970 (WKK05_31535) - 7388014..7388914 (+) 901 Protein_6336 IS982 family transposase -

Sequence

Protein


Download         Length: 373 a.a.        Molecular weight: 40833.15 Da        Isoelectric Point: 7.4768

>NTDB_id=974852 WKK05_RS31960 WP_341527010.1 7384133..7385254(-) (pilT) [Nostoc sp. UHCC 0302]
MEMMIEDLMEQLIEMGGSDMHLSAGLPPYFRISGKLTPIGEHVLTADQCQRLIFSMLNNTQRKTLEQTWELDCSYGVKGL
ARFRVNVYKERGAYAACLRALSSKIPNFEKLGLPDVVREMSDKPRGLILVTGPTGSGKTTTLAAMIDLINRTKAEHILTV
EDPIEFVYEPIKSLVHQRQLGEDTKSFANALKAALREDPDIVLVGEMRDLETISLAISAAETGHLVFGTLHTSSAAQTVD
RIIDVFPHERQTQVRVQLSNSLVAVFSQTLIPKKNPKPGEYGRVMAQEILIVTPAISNLIREGKTSQIYSAIQTGGKLGM
QTLEKVLADYYKAGTISFEAAMSKTSKPDEIQRLIGATAPPAGAKPGAAARAH

Nucleotide


Download         Length: 1122 bp        

>NTDB_id=974852 WKK05_RS31960 WP_341527010.1 7384133..7385254(-) (pilT) [Nostoc sp. UHCC 0302]
ATGGAAATGATGATTGAAGACCTGATGGAGCAATTGATTGAAATGGGTGGCTCGGATATGCATTTATCCGCAGGTTTGCC
TCCTTACTTCCGCATCAGTGGCAAACTCACCCCTATAGGTGAGCATGTACTAACGGCAGATCAGTGTCAAAGACTGATTT
TTAGTATGCTCAACAATACTCAGCGTAAAACCTTGGAACAGACTTGGGAGTTAGATTGTTCCTACGGTGTTAAAGGTTTA
GCTCGCTTCCGGGTGAATGTTTATAAAGAGCGTGGTGCATACGCTGCTTGCTTACGGGCATTAAGTTCTAAGATTCCTAA
CTTTGAAAAATTAGGTCTGCCAGATGTAGTGCGAGAAATGTCGGATAAGCCTAGAGGACTAATTCTGGTAACAGGCCCTA
CAGGTTCCGGAAAGACAACTACTTTAGCGGCAATGATTGACTTGATTAACCGCACCAAAGCAGAGCACATTTTAACGGTG
GAAGACCCGATTGAATTTGTTTATGAACCAATTAAAAGCTTAGTTCACCAACGACAACTGGGTGAAGATACTAAGAGCTT
TGCCAATGCTTTGAAAGCAGCTTTGCGGGAAGACCCAGATATTGTTCTGGTGGGAGAAATGCGCGATTTAGAAACGATTT
CTTTGGCAATTTCGGCTGCGGAAACAGGACACTTGGTATTTGGTACGCTACACACAAGTTCCGCTGCACAGACAGTTGAC
CGGATTATCGACGTTTTCCCCCATGAAAGACAAACCCAAGTGCGGGTGCAGTTATCTAACTCATTAGTAGCGGTATTTAG
CCAGACTTTGATACCTAAGAAAAACCCCAAACCCGGTGAGTATGGTCGGGTGATGGCTCAAGAAATTCTGATTGTCACTC
CTGCTATTTCTAACTTGATTCGAGAAGGCAAAACATCTCAAATTTACTCAGCTATTCAGACTGGTGGCAAATTGGGGATG
CAAACTCTGGAGAAGGTTTTAGCTGATTACTACAAAGCAGGAACAATCTCCTTTGAAGCGGCGATGTCTAAAACTTCTAA
GCCAGATGAAATCCAGCGCCTTATCGGTGCTACTGCACCACCAGCAGGTGCAAAACCTGGCGCAGCTGCTAGAGCGCATT
AA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(44-271)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Pseudomonas aeruginosa PAK

55.014

93.566

0.515
  pilT Pseudomonas stutzeri DSM 10701

53.868

93.566

0.504
  pilT Legionella pneumophila strain ERS1305867

52.45

93.029

0.488
  pilT Acinetobacter baylyi ADP1

52.45

93.029

0.488
  pilT Legionella pneumophila strain Lp02

52.45

93.029

0.488
  pilT Acinetobacter baumannii strain A118

52.161

93.029

0.485
  pilT Acinetobacter nosocomialis M2

52.161

93.029

0.485
  pilT Acinetobacter baumannii D1279779

52.161

93.029

0.485
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

48.857

93.834

0.458
  pilT Neisseria gonorrhoeae MS11

50.147

90.885

0.456
  pilT Neisseria meningitidis 8013

49.853

90.885

0.453
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.123

91.689

0.45
  pilT Vibrio cholerae strain A1552

49.123

91.689

0.45

Multiple sequence alignment