Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   WKK05_RS31965 Genome accession   NZ_CP151099
Coordinates   7385643..7387655 (-) Length   670 a.a.
NCBI ID   WP_341527011.1    Uniprot ID   -
Organism   Nostoc sp. UHCC 0302     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 7380643..7392655
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKK05_RS31945 (WKK05_31510) - 7381012..7382034 (+) 1023 WP_341527007.1 saccharopine dehydrogenase-like oxidoreductase -
  WKK05_RS31950 (WKK05_31515) - 7382100..7382660 (-) 561 WP_341527008.1 hypothetical protein -
  WKK05_RS31955 (WKK05_31520) - 7382834..7384057 (-) 1224 WP_341527009.1 type II secretion system F family protein -
  WKK05_RS31960 (WKK05_31525) pilT 7384133..7385254 (-) 1122 WP_341527010.1 type IV pilus twitching motility protein PilT Machinery gene
  WKK05_RS31965 (WKK05_31530) pilB/pilB1 7385643..7387655 (-) 2013 WP_341527011.1 ATPase, T2SS/T4P/T4SS family Machinery gene
  WKK05_RS31970 (WKK05_31535) - 7388014..7388914 (+) 901 Protein_6336 IS982 family transposase -
  WKK05_RS31980 (WKK05_31545) grpE 7390454..7391200 (+) 747 WP_341527012.1 nucleotide exchange factor GrpE -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 74774.70 Da        Isoelectric Point: 5.1761

>NTDB_id=974853 WKK05_RS31965 WP_341527011.1 7385643..7387655(-) (pilB/pilB1) [Nostoc sp. UHCC 0302]
MTSSSPQRRSTALTTRTEFSPFGNKLVQSGYINSEQMRQALIESRKSGIPLTEVLESITGQQLSPELLRQYKKQQLFELK
ILYGVEFLDPEVNQVGNTMVGNLIETLIPVDICRRHRLVPLSKQEDQKPPSVLVAMVAPDNLEASDELNRILRPQGLALQ
RMVITQEDYQQLINQYLDELAVRQKHLEQEKFTDINQDLENLGNLDISDAPEEMEADLGAAMKGAEDAPVINLVNRILAK
ALHEKVSDIHIEPQEENLRIRFRKDGVLREAFDPLPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRMFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLNKLITDPETLHIVQDMVSRPFGLILVTGPTGSGKTTSLYSALSEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFATALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSECRVPYTPTIEELARYGLSASSDVGITFYKANTLTLDAIAEAKSKNQLCPACNG
VGYKGRCGVYEVMRVTENLQTLINEEAPTERIKEVAVEEGMKTLLAYSLDLVRQGATTLEEVERVTFTDTGLEAELKAKR
KTGLTCRTCDATLKPEWLDCPYCMTSRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=974853 WKK05_RS31965 WP_341527011.1 7385643..7387655(-) (pilB/pilB1) [Nostoc sp. UHCC 0302]
ATGACTTCCTCGTCACCACAACGGCGCAGTACCGCTTTAACTACCAGAACAGAGTTTTCGCCTTTCGGCAACAAGCTAGT
GCAATCCGGCTATATCAATAGCGAACAGATGAGGCAAGCACTAATTGAAAGCCGCAAATCTGGCATACCGTTAACAGAAG
TATTAGAGTCAATTACTGGGCAACAACTATCACCTGAGTTGCTCAGGCAATACAAGAAACAGCAGCTATTTGAACTTAAA
ATACTATACGGAGTTGAATTCCTTGATCCGGAAGTCAACCAAGTTGGCAACACGATGGTGGGGAACCTAATTGAAACCCT
GATCCCAGTGGATATTTGTCGTCGCCATCGGTTAGTACCACTGTCGAAACAAGAGGATCAAAAACCGCCCTCAGTTTTAG
TGGCGATGGTCGCTCCGGATAATCTAGAGGCTTCTGATGAACTCAATCGCATCTTGCGTCCGCAAGGGTTAGCATTGCAG
CGCATGGTGATTACGCAGGAGGATTACCAGCAGCTAATTAACCAATACTTAGATGAACTGGCTGTTCGCCAAAAGCACCT
AGAACAAGAAAAGTTTACAGATATTAATCAGGATTTAGAAAATCTCGGAAATCTAGATATTTCAGATGCTCCTGAAGAAA
TGGAGGCTGATCTAGGGGCAGCGATGAAGGGTGCGGAGGATGCCCCAGTCATCAACCTAGTCAACAGAATCCTAGCTAAA
GCCTTGCATGAGAAGGTTTCTGATATTCATATCGAACCGCAAGAAGAAAACTTACGCATTCGCTTTCGGAAAGATGGGGT
ACTGCGTGAAGCTTTTGATCCCTTACCGAAAAAAATCATCCCGGCAGTAACAGCCCGATTTAAAATTATTTCTAATTTAG
ATATTGCCGAAAGACGTTTACCTCAAGATGGACGCATTCGGCGGATGTTTGAGGGACGTAAAGTGGACTTCCGTGTGAAC
ACCTTGCCCAGTCGCTACGGGGAAAAGGTGGTGCTGCGGATTTTGGATAACTCCTCTACCCAACTGGGATTGAATAAGTT
AATTACCGATCCGGAGACTTTGCATATTGTCCAAGATATGGTTAGCCGTCCCTTTGGTCTAATTTTGGTAACTGGGCCAA
CTGGTTCTGGAAAAACAACTTCGCTGTATTCTGCACTCTCAGAAAAGAACGATCCCGGAATTAATATCAGTACCGTAGAA
GACCCAATTGAGTACAGTCTTCCAGGGATTACTCAAGTACAGGTGATTCGGGAAAAAGGGCTGGATTTTGCTACCGCCCT
GCGGGCTTTCTTGCGGCAAGATCCAGATGTGCTGCTGGTGGGTGAAACACGGGACAAGGAAACGGCAAAAACAGCAATTG
AGGCTGCCTTGACCGGTCACTTAGTATTAACTACCTTACATACCAATGATGCCCCAGGTGCGATCGCTCGTTTGGGTGAA
ATGGGGATTGAGCCTTTCATGGTTTCTAGTTCCCTAATTGGCGTTTTAGCTCAACGTTTGGTGCGGCGTGTATGTTCTGA
ATGTCGTGTCCCCTACACGCCCACAATCGAGGAACTAGCTCGTTATGGTCTATCAGCTTCCTCTGATGTGGGAATTACCT
TCTATAAAGCTAACACCTTGACATTAGATGCGATCGCAGAAGCCAAAAGTAAAAATCAGCTTTGCCCAGCGTGTAATGGT
GTCGGCTACAAAGGACGTTGTGGCGTTTATGAAGTCATGCGAGTCACTGAAAATCTGCAAACTCTCATCAACGAGGAAGC
ACCCACGGAACGCATCAAAGAAGTGGCAGTAGAAGAGGGGATGAAAACCTTGCTGGCTTACAGTTTGGACTTAGTGCGCC
AAGGTGCTACCACCCTAGAAGAAGTAGAACGCGTGACCTTTACTGATACTGGTTTGGAAGCCGAGTTAAAAGCCAAACGT
AAGACTGGCCTTACCTGCCGGACTTGCGATGCCACATTGAAACCGGAATGGCTGGATTGCCCCTACTGTATGACATCCCG
GTTTCAAGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(236-503)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(27-179)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

65.625

100

0.658
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.569

95.97

0.37

Multiple sequence alignment