Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilAI   Type   Machinery gene
Locus tag   NSQ78_RS17080 Genome accession   NZ_CP150199
Coordinates   3664866..3665294 (-) Length   142 a.a.
NCBI ID   WP_149901976.1    Uniprot ID   -
Organism   Stenotrophomonas sp. FSL W7-1447     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3659866..3670294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ78_RS17065 (NSQ78_17065) pilR 3660479..3661852 (+) 1374 WP_049471425.1 sigma-54 dependent transcriptional regulator Regulator
  NSQ78_RS17070 (NSQ78_17070) pilB 3661947..3663677 (-) 1731 WP_053506935.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NSQ78_RS17075 (NSQ78_17075) - 3663838..3664671 (-) 834 WP_064239075.1 sterol desaturase family protein -
  NSQ78_RS17080 (NSQ78_17080) pilA/pilAI 3664866..3665294 (-) 429 WP_149901976.1 pilin Machinery gene
  NSQ78_RS17085 (NSQ78_17085) pilC 3665649..3666908 (+) 1260 WP_064239077.1 type II secretion system F family protein Machinery gene
  NSQ78_RS17090 (NSQ78_17090) - 3666912..3667775 (+) 864 WP_064239078.1 A24 family peptidase -
  NSQ78_RS17095 (NSQ78_17095) coaE 3667787..3668398 (+) 612 WP_064239079.1 dephospho-CoA kinase -
  NSQ78_RS17100 (NSQ78_17100) - 3669214..3669810 (-) 597 WP_149901977.1 hypothetical protein -

Sequence

Protein


Download         Length: 142 a.a.        Molecular weight: 14652.68 Da        Isoelectric Point: 8.4832

>NTDB_id=966061 NSQ78_RS17080 WP_149901976.1 3664866..3665294(-) (pilA/pilAI) [Stenotrophomonas sp. FSL W7-1447]
MKNQKGFTLIELMIVVAIIAILAAIAIPAYQDYVVRSQAASALAEITPGKVGFEQATNEGKTPSTTATDAGFIGVAASTS
YCNVAVTATTIACTTKNGNATKFNTKNITWTRDANSGLWSCSTSLDAKYRPGQCGAAAAPAP

Nucleotide


Download         Length: 429 bp        

>NTDB_id=966061 NSQ78_RS17080 WP_149901976.1 3664866..3665294(-) (pilA/pilAI) [Stenotrophomonas sp. FSL W7-1447]
ATGAAGAACCAGAAGGGCTTCACCCTTATCGAACTGATGATCGTCGTTGCGATCATCGCCATTTTGGCTGCGATTGCGAT
CCCGGCCTATCAGGACTACGTCGTTCGTTCGCAGGCGGCTTCCGCCCTGGCTGAGATTACCCCCGGCAAGGTCGGCTTCG
AACAGGCGACCAACGAAGGCAAGACTCCGTCGACCACCGCAACCGATGCGGGCTTCATCGGCGTTGCTGCCAGCACCTCC
TACTGCAATGTGGCCGTGACTGCCACGACCATTGCCTGCACCACTAAGAACGGCAACGCTACCAAGTTCAACACCAAGAA
CATTACGTGGACCCGCGATGCCAACAGCGGCCTGTGGTCCTGCTCCACCAGCCTCGACGCGAAGTATCGTCCGGGCCAGT
GTGGTGCCGCCGCCGCTCCGGCGCCGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-134)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilAI Pseudomonas stutzeri DSM 10701

68.657

94.366

0.648
  pilA/pilAII Pseudomonas stutzeri DSM 10701

60.902

93.662

0.57
  pilA Acinetobacter baumannii strain A118

47.586

100

0.486
  pilA Pseudomonas aeruginosa PAK

45.946

100

0.479
  comP Acinetobacter baylyi ADP1

44.521

100

0.458
  pilA Vibrio cholerae strain A1552

44.138

100

0.451
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.138

100

0.451
  pilA Vibrio cholerae C6706

44.138

100

0.451
  pilA Ralstonia pseudosolanacearum GMI1000

37.654

100

0.43
  pilE Neisseria gonorrhoeae MS11

34.731

100

0.408
  pilA2 Legionella pneumophila str. Paris

42.336

96.479

0.408
  pilA2 Legionella pneumophila strain ERS1305867

42.336

96.479

0.408

Multiple sequence alignment