Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NSQ78_RS17070 Genome accession   NZ_CP150199
Coordinates   3661947..3663677 (-) Length   576 a.a.
NCBI ID   WP_053506935.1    Uniprot ID   -
Organism   Stenotrophomonas sp. FSL W7-1447     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3656947..3668677
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NSQ78_RS17055 (NSQ78_17055) sucC 3657234..3658403 (-) 1170 WP_005418614.1 ADP-forming succinate--CoA ligase subunit beta -
  NSQ78_RS17060 (NSQ78_17060) - 3658811..3660424 (+) 1614 WP_049471427.1 ATP-binding protein -
  NSQ78_RS17065 (NSQ78_17065) pilR 3660479..3661852 (+) 1374 WP_049471425.1 sigma-54 dependent transcriptional regulator Regulator
  NSQ78_RS17070 (NSQ78_17070) pilB 3661947..3663677 (-) 1731 WP_053506935.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NSQ78_RS17075 (NSQ78_17075) - 3663838..3664671 (-) 834 WP_064239075.1 sterol desaturase family protein -
  NSQ78_RS17080 (NSQ78_17080) pilA/pilAI 3664866..3665294 (-) 429 WP_149901976.1 pilin Machinery gene
  NSQ78_RS17085 (NSQ78_17085) pilC 3665649..3666908 (+) 1260 WP_064239077.1 type II secretion system F family protein Machinery gene
  NSQ78_RS17090 (NSQ78_17090) - 3666912..3667775 (+) 864 WP_064239078.1 A24 family peptidase -
  NSQ78_RS17095 (NSQ78_17095) coaE 3667787..3668398 (+) 612 WP_064239079.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 576 a.a.        Molecular weight: 62227.11 Da        Isoelectric Point: 5.8722

>NTDB_id=966060 NSQ78_RS17070 WP_053506935.1 3661947..3663677(-) (pilB) [Stenotrophomonas sp. FSL W7-1447]
MNTVTTANLVGITGLARRLVQDGALDEASARDAMAKAAAARQPLPTWFAQNKVVGAAQLAAANAVEFGMPLFDVSTFDAS
QNAMSLVSEELLRKHNVLPLFKRGGKLFVGTSNPTHSLDEIKFHTNLVVEPILVDEDQIRRTLEQWHASHDTLGDALGGD
DDGIGNLDVSGGDEDISGGGDSGIDAKGDDTPVVKFVNKVLVDAIRKGASDIHFEPYEDDYRVRLRIDGLLKMVARAPVK
LNQRIAARLKVMAQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEPDQQKLF
LDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPDI
IMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRPANLPEH
ALLAEGFTQAQLDAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIATIVLAGGNALQIAEAAQQIGVNDLRQSALKKA
AAGITSLAEINRVTKD

Nucleotide


Download         Length: 1731 bp        

>NTDB_id=966060 NSQ78_RS17070 WP_053506935.1 3661947..3663677(-) (pilB) [Stenotrophomonas sp. FSL W7-1447]
ATGAACACCGTAACCACCGCCAATCTCGTCGGCATCACCGGCCTGGCCCGTCGCCTGGTCCAGGATGGCGCCCTGGACGA
GGCTTCTGCCCGTGACGCCATGGCCAAGGCCGCTGCGGCCCGCCAGCCGCTGCCGACCTGGTTCGCGCAGAACAAGGTGG
TCGGGGCGGCCCAGTTGGCGGCCGCCAACGCGGTCGAGTTCGGCATGCCGCTGTTCGACGTGTCCACGTTCGACGCCAGC
CAGAACGCAATGAGCCTGGTCAGCGAGGAACTGCTGCGCAAGCACAACGTGCTGCCGCTGTTCAAACGCGGCGGCAAGCT
GTTCGTGGGCACCAGCAATCCGACCCATTCGCTGGACGAGATCAAGTTCCACACCAACCTGGTGGTGGAGCCGATCCTGG
TGGATGAAGACCAGATCCGCCGCACGCTCGAGCAGTGGCATGCCAGCCATGACACGCTGGGCGACGCGCTGGGCGGTGAC
GACGATGGCATTGGCAACCTGGATGTGTCCGGCGGTGACGAGGACATTTCCGGGGGCGGTGACTCCGGCATCGACGCCAA
GGGCGACGACACCCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGACGCGATCCGCAAGGGCGCCTCGGACATCCACT
TCGAGCCCTATGAAGACGACTACCGCGTGCGCCTGCGCATTGATGGCCTGCTGAAGATGGTCGCGCGCGCGCCGGTGAAG
CTGAACCAGCGTATCGCCGCACGCCTGAAGGTGATGGCGCAGCTGGACATCGCCGAGAAGCGCGTGCCGCAGGACGGACG
CATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCCTGCCGACCCTGTTCGGCGAGAAGGTGG
TGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGACAAGCTGGGGTACGAGCCGGACCAGCAGAAGCTGTTC
CTGGACGCCATCCACAAGCCCTACGGCATGGTGCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTGTACAC
CGCGCTGGGCATCCTCAACGACGAGACGCGTAACATCTCCACCGCCGAAGACCCGGTGGAAATCCGCTTGCCCGGCGTCA
ACCAGGTGCAGCAGAACAACAAGCGCGGCATGACCTTCGCCGCGGCACTGCGCTCGTTCCTGCGACAGGACCCGGACATC
ATCATGGTCGGCGAAATCCGCGACCTGGAGACGGCCGAGATCGCGATCAAGGCGGCCCAGACCGGCCACATGGTGCTGTC
CACGCTGCATACCAACGATGCGCCGCAGACCATCGCGCGCCTGATGAACATGGGCATTGCGCCGTACAACATCACCAGTT
CGGTGACGCTGGTGATTGCCCAGCGCCTGGCGCGCCGGTTGTGCAACAACTGCAAGCGCCCGGCCAACCTGCCGGAGCAC
GCGCTGCTGGCTGAAGGCTTCACCCAGGCGCAGCTGGATGCGGGTATCGAACTGTACGAGGCGGTCGGCTGTGACGAATG
CACCGAGGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATCGCCACGATCGTGCTGGCCG
GTGGCAATGCGTTGCAGATTGCCGAGGCCGCGCAGCAGATCGGGGTGAACGACCTGCGCCAGTCGGCGTTGAAGAAGGCG
GCGGCCGGGATCACCAGCCTGGCCGAGATCAACCGCGTTACCAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(49-150)

Type II/IV secretion system protein (T2SSE)

(199-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.634

98.611

0.549
  pilB Acinetobacter baylyi ADP1

54.799

99.479

0.545
  pilB Legionella pneumophila strain ERS1305867

52.021

98.785

0.514
  pilF Neisseria gonorrhoeae MS11

48.944

98.611

0.483
  pilB Vibrio cholerae strain A1552

48.601

99.306

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

45.629

99.306

0.453
  pilB Vibrio campbellii strain DS40M4

45.518

98.785

0.45

Multiple sequence alignment