Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   DAETH_RS00965 Genome accession   NZ_AP026560
Coordinates   200761..202008 (+) Length   415 a.a.
NCBI ID   WP_264776093.1    Uniprot ID   -
Organism   Deinococcus aetherius strain ST0316     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 195761..207008
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DAETH_RS00945 (DAETH_01840) - 196089..196388 (-) 300 WP_264774762.1 transposase -
  DAETH_RS00950 (DAETH_01860) pgeF 196646..197419 (+) 774 WP_264776090.1 peptidoglycan editing factor PgeF -
  DAETH_RS00955 (DAETH_01870) - 197416..197934 (+) 519 WP_264776091.1 YqeG family HAD IIIA-type phosphatase -
  DAETH_RS00960 (DAETH_01880) pilB 198017..200689 (+) 2673 WP_264776092.1 type II/IV secretion system protein Machinery gene
  DAETH_RS00965 (DAETH_01890) pilT 200761..202008 (+) 1248 WP_264776093.1 type IV pilus twitching motility protein PilT Machinery gene
  DAETH_RS00970 (DAETH_01900) - 202054..202671 (+) 618 WP_319993725.1 histidine phosphatase family protein -
  DAETH_RS00975 (DAETH_01910) - 202695..203120 (+) 426 WP_264776095.1 secondary thiamine-phosphate synthase enzyme YjbQ -
  DAETH_RS00980 (DAETH_01920) - 203133..205511 (-) 2379 WP_264776096.1 penicillin acylase family protein -
  DAETH_RS00985 (DAETH_01930) - 205557..206435 (-) 879 WP_264776097.1 DMT family transporter -

Sequence

Protein


Download         Length: 415 a.a.        Molecular weight: 44388.86 Da        Isoelectric Point: 9.0950

>NTDB_id=96395 DAETH_RS00965 WP_264776093.1 200761..202008(+) (pilT) [Deinococcus aetherius strain ST0316]
MTNPAPADITDILRVAAEKGASDVIITVGLPPQFKLQGVYDPQGFSELVPTQTRKLMYSMMNEKQQRTFEEKRELDFSFA
LGEKARFRVNAFMQRGNVGGVLRLIPTKIKTAQEMGLPQSIVELGLAPRGLVLVTGPTGSGKSTTLAAIIDHINQTKRAH
IVTIEDPIEFMHTHKQSIVNQREVGADTMSFNAALRAALRQAPDVILVGEMRDYETIKAAVTAAETGHLVMGTLHTNSAP
ESIDRIVDVFPEEQQEQIRVQLAGNLVAVMTQQLLPKADGSGRILAYELLIANPAVRSLIREGKTFQIVSTMQTGAREGM
VTMDAFLANLYRRRVITYDVGVERAVDPKEFARLANDPGAGTGAAAGYAPPAGGGAMPQGAGRAAANPTLSGVPTGRTVA
DAGFGTGGGKPYGRG

Nucleotide


Download         Length: 1248 bp        

>NTDB_id=96395 DAETH_RS00965 WP_264776093.1 200761..202008(+) (pilT) [Deinococcus aetherius strain ST0316]
ATGACCAATCCCGCCCCCGCCGACATCACCGATATCCTGCGCGTGGCCGCCGAGAAGGGCGCGTCCGACGTGATCATCAC
CGTGGGGCTGCCGCCGCAGTTCAAGCTCCAGGGGGTGTACGACCCGCAGGGCTTTTCCGAACTCGTCCCGACCCAGACGC
GCAAGCTGATGTACTCGATGATGAACGAGAAGCAGCAGCGCACCTTCGAGGAGAAACGCGAGCTTGACTTCTCCTTCGCG
CTGGGTGAAAAGGCCCGTTTCCGCGTGAATGCCTTTATGCAGCGCGGGAACGTGGGCGGGGTGCTGCGCCTGATCCCGAC
GAAGATCAAGACCGCACAGGAGATGGGGCTGCCGCAGAGCATCGTGGAACTGGGGCTGGCGCCGCGCGGGCTGGTGCTGG
TGACCGGCCCGACGGGCTCGGGCAAGAGCACCACGCTCGCCGCGATCATCGACCACATCAACCAGACGAAGCGGGCGCAC
ATCGTAACCATCGAGGACCCCATCGAGTTCATGCACACGCACAAGCAGAGCATCGTGAACCAGCGCGAGGTGGGGGCCGA
CACGATGAGTTTCAACGCCGCCCTGCGCGCCGCGCTTCGTCAGGCGCCCGACGTGATCCTGGTGGGCGAGATGCGCGACT
ACGAGACGATCAAGGCCGCCGTCACCGCCGCCGAGACCGGGCACCTCGTGATGGGCACCCTGCACACCAACTCGGCCCCC
GAGAGCATCGACCGCATCGTGGACGTGTTCCCGGAAGAGCAGCAGGAGCAGATCCGGGTGCAGCTCGCGGGCAACCTCGT
CGCGGTGATGACCCAGCAGCTCCTCCCCAAGGCGGACGGCTCAGGCCGCATCCTCGCCTACGAACTCCTGATCGCCAACC
CCGCCGTGCGCTCCCTGATCCGCGAGGGCAAGACCTTCCAGATCGTCTCGACCATGCAGACGGGCGCCCGCGAGGGCATG
GTCACGATGGACGCCTTCCTGGCGAACCTCTACCGCCGCCGCGTGATCACCTACGACGTCGGCGTCGAGCGCGCCGTGGA
CCCCAAGGAGTTCGCCCGCCTCGCCAACGACCCGGGCGCGGGTACCGGGGCCGCCGCCGGGTACGCTCCCCCGGCGGGCG
GCGGGGCCATGCCCCAGGGGGCGGGCCGCGCCGCCGCCAACCCCACCCTGTCGGGCGTGCCCACGGGCCGCACCGTCGCC
GACGCGGGCTTCGGGACGGGCGGGGGCAAGCCGTACGGGCGGGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(43-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

80.601

88.193

0.711
  pilT Pseudomonas stutzeri DSM 10701

51.453

82.892

0.427
  pilT Pseudomonas aeruginosa PAK

51.163

82.892

0.424
  pilT Acinetobacter nosocomialis M2

50.872

82.892

0.422
  pilT Acinetobacter baumannii D1279779

50.872

82.892

0.422
  pilT Acinetobacter baumannii strain A118

50.872

82.892

0.422
  pilT Acinetobacter baylyi ADP1

50

82.892

0.414
  pilT Neisseria meningitidis 8013

49.425

83.855

0.414
  pilT Legionella pneumophila strain Lp02

48.837

82.892

0.405
  pilT Legionella pneumophila strain ERS1305867

48.837

82.892

0.405
  pilT Neisseria gonorrhoeae MS11

48.837

82.892

0.405
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

48.024

79.277

0.381
  pilT Vibrio cholerae strain A1552

48.024

79.277

0.381
  pilU Pseudomonas stutzeri DSM 10701

46.951

79.036

0.371

Multiple sequence alignment