Detailed information NTG0096394

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: DAETH_RS00960
• Genome accession: NZ_AP026560
• Coordinates: 198017..200689 (+)
• Length: 890 a.a.
• NCBI ID: WP_264776092.1
• Uniprot ID: -
• Organism: Deinococcus aetherius strain ST0316
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 193017..205689

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DAETH_RS00930 (DAETH_01810)	-	193781..194026 (-)	246	Protein_181	transposase	-
DAETH_RS00935 (DAETH_01820)	-	194070..195473 (-)	1404	WP_264774760.1	IS30 family transposase	-
DAETH_RS00940 (DAETH_01830)	-	195445..196092 (-)	648	WP_264774761.1	IS3 family transposase	-
DAETH_RS00945 (DAETH_01840)	-	196089..196388 (-)	300	WP_264774762.1	transposase	-
DAETH_RS00950 (DAETH_01860)	pgeF	196646..197419 (+)	774	WP_264776090.1	peptidoglycan editing factor PgeF	-
DAETH_RS00955 (DAETH_01870)	-	197416..197934 (+)	519	WP_264776091.1	YqeG family HAD IIIA-type phosphatase	-
DAETH_RS00960 (DAETH_01880)	pilB	198017..200689 (+)	2673	WP_264776092.1	type II/IV secretion system protein	Machinery gene
DAETH_RS00965 (DAETH_01890)	pilT	200761..202008 (+)	1248	WP_264776093.1	type IV pilus twitching motility protein PilT	Machinery gene
DAETH_RS00970 (DAETH_01900)	-	202054..202671 (+)	618	WP_319993725.1	histidine phosphatase family protein	-
DAETH_RS00975 (DAETH_01910)	-	202695..203120 (+)	426	WP_264776095.1	secondary thiamine-phosphate synthase enzyme YjbQ	-
DAETH_RS00980 (DAETH_01920)	-	203133..205511 (-)	2379	WP_264776096.1	penicillin acylase family protein	-

Sequence

Protein

• Length: 890 a.a.
• Molecular weight: 96548.49 Da
• Isoelectric Point: 4.9433

>NTDB_id=96394 DAETH_RS00960 WP_264776092.1 198017..200689(+) (pilB) [Deinococcus aetherius strain ST0316]
MALSIGDRRLGAILLEQGYVNDLDLQKALVRHAEVGGRLSDILIDSGMVGEKRIARAIEEALGIPLVDLLVITPEPAAVA
AISAQTAQTVQAFPFALDGETLRVALADPLSSVAIEALEDDSGLMIEPYQALRDQILWSIATHYPELDLSAPMPAESQGD
SAGGGRLGQRLIARGLLNESQLGVALDVQQQTGEPLGNTLVTQGILNEDQLYEVLAEQAGVAFVRNPRDFQPTEDVLGGM
LRADALRLQAVPVDEGPQGVVAVASDPRKRDDLAAVLGRPVQLVLAKPRDVEALIERFYPQRGRLGEQMVQQGTLSRAQL
REALQVQARGGKVKPLGEVILELGFAKADEIEGALQKQNAGGGRLEDTLVQSGKLSPEMLARSLAAQLGYEFLDPARNPP
DAKVALMIPESTARRYLVVPVRFQGESLVIAMKDPRNVFALDDLKLITGREIVPAVMAEKDITRLIERYFGSQDMADLGK
RLAAESKSREAKKQEDVDVSALDDNAVVRVVDNLIREAALMEASDIHIEPTETSLRVRYRIDGVLREQNELPRGSAQSIL
ARIKIMGSLDISERRVPQDGRIRFKKGSIDIDLRLSTLPTVYGEKAVMRLLQKASNIPEVEQLGFSEHNFQRYLDVTDKP
NGIFLITGPTGSGKSFTSFSTLKRIARPEKNTTTIEDPIEYEIPGIVQSQVNVAAGMTFARALRAFLRQDPDIIFVGEVR
DVETAKIATEAALTGHLVLATLHTNDAPGAITRLEEMGVEHFNIAAAVVGVLAQRLVRRVCGECKQSTNADPNVLRRLGL
TEAQVSGGNLVRGSGCPRCGGTGYKGRMGIHELMVIDDPLRRAIGAGRTAAEIRDVALAESEMRTLRQDGIEKALQGLTT
LEEVLAVTSN

Nucleotide

• Length: 2673 bp

>NTDB_id=96394 DAETH_RS00960 WP_264776092.1 198017..200689(+) (pilB) [Deinococcus aetherius strain ST0316]
GTGGCACTCTCGATAGGTGACCGGCGCCTCGGCGCAATCCTGCTGGAGCAGGGGTACGTGAACGACCTCGACCTGCAAAA
GGCGCTCGTGCGCCACGCGGAGGTGGGGGGTCGTCTCAGCGACATCCTGATCGACTCGGGGATGGTGGGCGAGAAACGCA
TCGCGCGGGCCATCGAGGAGGCGCTGGGCATTCCCCTGGTGGACCTGCTGGTCATCACGCCCGAGCCAGCGGCGGTCGCG
GCGATCAGTGCCCAGACGGCGCAGACGGTGCAGGCCTTCCCCTTCGCGCTGGACGGCGAGACGCTGCGGGTGGCCCTGGC
CGACCCCCTCTCCAGCGTGGCCATCGAGGCGCTGGAGGACGACAGCGGGCTGATGATCGAGCCGTACCAGGCGCTGCGCG
ACCAGATCCTGTGGTCCATCGCCACCCATTACCCGGAGCTGGACCTCTCGGCCCCCATGCCTGCCGAGTCGCAGGGGGAC
AGCGCGGGGGGTGGGCGGCTCGGGCAGCGCCTGATCGCTCGGGGCCTCCTGAACGAGTCGCAGCTTGGGGTCGCGCTCGA
CGTGCAGCAGCAGACCGGCGAGCCGCTGGGCAACACCCTGGTCACCCAGGGAATCCTCAACGAGGACCAGCTCTACGAGG
TTCTGGCCGAGCAGGCTGGGGTCGCTTTCGTGCGCAACCCGCGCGACTTCCAACCCACCGAGGACGTGCTGGGCGGGATG
CTGCGCGCGGACGCCCTGCGACTCCAGGCCGTGCCGGTGGACGAGGGCCCGCAGGGCGTGGTCGCCGTGGCGAGCGACCC
CCGAAAGCGCGACGACCTCGCGGCGGTGCTCGGGCGCCCGGTGCAGCTCGTCCTCGCCAAGCCGCGCGACGTGGAGGCCC
TGATCGAGCGCTTCTACCCGCAGCGTGGCCGCCTGGGCGAGCAGATGGTCCAGCAGGGGACCCTCTCGCGGGCGCAACTG
CGCGAAGCCCTTCAGGTTCAGGCGCGCGGCGGCAAGGTCAAGCCGCTCGGCGAGGTGATTCTCGAACTGGGCTTCGCCAA
GGCGGACGAGATCGAGGGGGCGTTGCAAAAGCAGAACGCGGGCGGGGGCCGCCTGGAGGATACCCTCGTCCAGTCGGGCA
AGCTCAGCCCCGAGATGCTCGCCCGCTCGCTCGCCGCACAGCTCGGCTACGAGTTCCTCGATCCCGCCCGGAATCCGCCC
GACGCCAAGGTCGCGCTGATGATCCCGGAGTCGACGGCGCGGCGCTACCTGGTGGTGCCCGTGCGGTTCCAGGGCGAATC
GCTCGTCATCGCCATGAAGGACCCGCGCAACGTCTTCGCGCTGGACGACCTCAAGCTCATCACGGGGCGCGAAATCGTCC
CGGCGGTGATGGCGGAGAAAGACATCACCCGGCTGATCGAGCGCTACTTCGGCAGTCAGGACATGGCCGACCTGGGCAAG
AGGCTCGCCGCCGAGAGCAAGAGCCGCGAGGCCAAGAAGCAGGAGGACGTGGACGTCTCCGCCCTCGACGACAACGCCGT
CGTCCGGGTGGTGGACAACCTGATCCGCGAGGCCGCCCTGATGGAGGCCTCCGACATCCACATCGAGCCCACCGAGACGT
CCCTGCGGGTGCGCTACCGCATCGACGGCGTGCTGCGCGAGCAGAACGAGCTGCCCAGGGGAAGCGCCCAGAGCATCCTC
GCCCGCATCAAGATCATGGGAAGCCTCGACATCTCCGAGCGCCGGGTGCCCCAGGACGGCCGCATCCGCTTCAAGAAGGG
TTCCATCGACATCGACCTGCGCCTCTCGACCCTGCCCACCGTGTACGGCGAGAAGGCCGTGATGCGCCTGCTGCAAAAGG
CGAGCAACATCCCGGAGGTCGAGCAGCTCGGCTTCTCCGAGCACAACTTCCAGCGGTATCTGGACGTGACGGACAAGCCC
AACGGCATCTTCCTGATCACCGGGCCGACGGGCTCGGGCAAGTCCTTCACCTCCTTTTCGACCCTCAAGCGCATCGCCCG
CCCCGAGAAGAACACGACGACCATCGAGGACCCCATCGAGTACGAGATTCCGGGCATCGTGCAGTCGCAGGTCAACGTGG
CGGCAGGGATGACCTTCGCGCGGGCGCTGCGGGCCTTCCTGCGCCAGGACCCCGACATCATCTTCGTGGGCGAGGTCCGC
GACGTGGAGACCGCCAAGATCGCCACCGAGGCCGCGCTGACCGGCCACCTCGTGCTGGCGACCCTGCACACGAACGACGC
CCCCGGCGCGATCACGAGGTTGGAGGAGATGGGCGTGGAGCACTTCAACATCGCCGCCGCCGTGGTGGGCGTGCTCGCTC
AGCGCCTCGTCAGAAGGGTGTGCGGCGAGTGCAAGCAGTCCACGAACGCCGACCCCAACGTCCTGCGCCGCCTGGGGCTG
ACCGAGGCCCAGGTCAGCGGCGGCAACCTCGTGCGCGGCTCGGGCTGCCCCCGTTGCGGCGGCACGGGCTACAAGGGCCG
TATGGGCATCCACGAACTCATGGTGATCGACGATCCCCTGCGCCGCGCCATCGGGGCGGGGAGGACCGCCGCCGAGATCC
GCGACGTGGCCCTGGCCGAGAGCGAGATGCGGACCCTCCGGCAAGACGGCATCGAGAAGGCCTTGCAGGGGCTGACCACC
CTGGAGGAAGTGCTGGCGGTGACGAGCAACTAG

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (369-474)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (200-303)

• Type II/IV secretion system protein (T2SSE) (514-779)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	75.618	100	0.756
pilF	Thermus thermophilus HB27	54.688	100	0.551