Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WKV34_RS18865 Genome accession   NZ_CP149825
Coordinates   3917796..3918776 (-) Length   326 a.a.
NCBI ID   WP_060572195.1    Uniprot ID   A0A8E1RQ99
Organism   Enterobacter bugandensis strain 2024CK-00243     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3912796..3923776
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WKV34_RS18835 (WKV34_18835) - 3913678..3914175 (+) 498 WP_028014364.1 SprT family zinc-dependent metalloprotease -
  WKV34_RS18840 (WKV34_18840) endA 3914270..3914977 (+) 708 WP_028014365.1 deoxyribonuclease I -
  WKV34_RS18845 (WKV34_18845) rsmE 3915029..3915760 (+) 732 WP_041908327.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  WKV34_RS18850 (WKV34_18850) gshB 3915780..3916727 (+) 948 WP_033146605.1 glutathione synthase -
  WKV34_RS18855 (WKV34_18855) - 3916810..3917370 (+) 561 WP_028014368.1 YqgE/AlgH family protein -
  WKV34_RS18860 (WKV34_18860) ruvX 3917370..3917786 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  WKV34_RS18865 (WKV34_18865) pilT 3917796..3918776 (-) 981 WP_060572195.1 type IV pilus twitching motility protein PilT Machinery gene
  WKV34_RS18870 (WKV34_18870) - 3918794..3919495 (+) 702 WP_047364543.1 YggS family pyridoxal phosphate-dependent enzyme -
  WKV34_RS18875 (WKV34_18875) - 3919517..3920083 (+) 567 WP_045260768.1 YggT family protein -
  WKV34_RS18880 (WKV34_18880) yggU 3920080..3920376 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  WKV34_RS18885 (WKV34_18885) - 3920380..3920973 (+) 594 WP_010435473.1 XTP/dITP diphosphatase -
  WKV34_RS18890 (WKV34_18890) hemW 3920966..3922108 (+) 1143 WP_047364547.1 radical SAM family heme chaperone HemW -
  WKV34_RS18895 (WKV34_18895) - 3922172..3922510 (+) 339 WP_028014373.1 endonuclease domain-containing protein -
  WKV34_RS18900 (WKV34_18900) - 3922581..3923297 (-) 717 WP_041908321.1 DUF2884 domain-containing protein -
  WKV34_RS18905 (WKV34_18905) - 3923355..3923681 (-) 327 WP_028014375.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35601.69 Da        Isoelectric Point: 6.3904

>NTDB_id=961748 WKV34_RS18865 WP_060572195.1 3917796..3918776(-) (pilT) [Enterobacter bugandensis strain 2024CK-00243]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVAGDQRLRG
SAFKQMKGISVTLRLLPRSCPQLPSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNRHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSMRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=961748 WKV34_RS18865 WP_060572195.1 3917796..3918776(-) (pilT) [Enterobacter bugandensis strain 2024CK-00243]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTGGGCCGCCTTGAGCCGGCCCCGTTTCCGCCGCCGGACGTCGGGGCGTTGCTAAAGGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTCGCAGGAGACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCATCTCCGTTACCCTGCGGCTGTTGCCGCGTTCCTGTCCGCAGCTCCCTTCGCTTGG
GGTGCCGCGCGCTATTCCCGAACTGTTGTCCAGCGACAACGGGTTGATTCTGGTAACGGGAGCGACCGGCAGCGGTAAAT
CCACCACGCTGGCCGCAATGGTCGACTTCCTCAACCGCCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCAGAGGCGCTGCG
CAGTGCGTTACGCGAGGACCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGGGCAGCACAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCATGCGGGCAGTGCTGGCGCAAAAGCTGCTTCC
CGATCTTCAGGGCGGGCGCGTCGCGCTGTACGAGCTGCTGGTAAACACCGCGGCGGCGGCGAATCTGATTCGTGAGGGGA
AGACGTGGCAGCTGCCCGGCATCATTCAAACCGGCCAGCAGGCGGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCACAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Acinetobacter baumannii strain A118

48.012

100

0.482
  pilT Acinetobacter baumannii D1279779

48.012

100

0.482
  pilT Acinetobacter baylyi ADP1

48.012

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Acinetobacter nosocomialis M2

48.438

98.16

0.475
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.547

98.773

0.42
  pilU Pseudomonas stutzeri DSM 10701

38.788

100

0.393
  pilU Vibrio cholerae strain A1552

39.13

98.773

0.386