Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   WCY31_RS06795 Genome accession   NZ_CP147920
Coordinates   1328586..1329662 (+) Length   358 a.a.
NCBI ID   WP_345969075.1    Uniprot ID   -
Organism   Sulfurimonas sp. HSL3-1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1323586..1334662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WCY31_RS06775 (WCY31_06775) - 1324894..1327401 (-) 2508 WP_345971781.1 sugar phosphate nucleotidyltransferase -
  WCY31_RS06780 (WCY31_06780) - 1327495..1327776 (-) 282 WP_345969072.1 hypothetical protein -
  WCY31_RS06785 (WCY31_06785) gatC 1327955..1328245 (+) 291 WP_345969073.1 Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC -
  WCY31_RS06790 (WCY31_06790) - 1328242..1328589 (+) 348 WP_345971783.1 arsenate reductase family protein -
  WCY31_RS06795 (WCY31_06795) pilT 1328586..1329662 (+) 1077 WP_345969075.1 type IV pilus twitching motility protein PilT Machinery gene
  WCY31_RS06800 (WCY31_06800) - 1329757..1331106 (-) 1350 WP_345971785.1 3-deoxy-7-phosphoheptulonate synthase class II -
  WCY31_RS06805 (WCY31_06805) - 1331257..1331859 (+) 603 WP_345969077.1 CvpA family protein -
  WCY31_RS06810 (WCY31_06810) - 1331856..1332338 (+) 483 WP_345969078.1 Fur family transcriptional regulator -
  WCY31_RS06815 (WCY31_06815) lysS 1332354..1333910 (+) 1557 WP_345971787.1 lysine--tRNA ligase -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39875.85 Da        Isoelectric Point: 6.1120

>NTDB_id=949754 WCY31_RS06795 WP_345969075.1 1328586..1329662(+) (pilT) [Sulfurimonas sp. HSL3-1]
MSQTLDIRKLLKSVVAYNASDLHLVSRSEPQIRIDGRLVALNLPVLDGKTIEDMSYSLLTEKQKKEFEEHNELDFAILLP
EVGRFRANYYKTMGDIACAFRIIPIEIPSLDDLNAKPIFKKLVKREKGLILVTGPTGSGKSTTLAAMLNEINETENRHII
SVEDPVEFVHTNKQCLFSHRNVGTDTANFANALKYAMREDPDVILVGEMRDKETIAAALTAAETGHLVFGTLHTNSAPQT
INRIIDVFSGDEQPQVRAQLSTSIIAIIAQALLPRLGGGRVAAQELMINIPAIANLIREDKVHQLYSQMQLNQQETGMVT
QTQELFDLVRKRVISKQDAIQWSNRPDELIKALDAAAL

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=949754 WCY31_RS06795 WP_345969075.1 1328586..1329662(+) (pilT) [Sulfurimonas sp. HSL3-1]
ATGAGTCAGACCCTGGATATCCGTAAACTATTAAAAAGCGTCGTTGCCTACAATGCATCGGACCTTCACCTCGTTTCCCG
TTCCGAACCGCAGATCCGTATCGACGGGCGCCTGGTCGCACTGAACCTTCCCGTCCTCGACGGCAAAACAATTGAGGATA
TGTCCTACTCCCTGCTGACAGAGAAACAGAAGAAAGAGTTCGAAGAGCATAACGAACTCGACTTCGCGATCCTGCTCCCC
GAAGTCGGCCGTTTCCGTGCGAACTACTATAAAACGATGGGCGACATCGCCTGCGCCTTCCGTATCATTCCGATCGAGAT
CCCGAGCCTGGACGATCTCAATGCCAAGCCGATCTTCAAAAAACTCGTCAAACGCGAAAAAGGACTGATTCTCGTCACCG
GACCGACCGGTAGCGGTAAGTCCACCACCCTGGCCGCGATGCTCAACGAGATCAACGAAACGGAAAACCGCCACATTATC
AGTGTCGAGGACCCGGTCGAATTCGTCCACACCAACAAGCAGTGTCTCTTCTCCCACCGTAACGTCGGGACCGATACCGC
CAACTTCGCCAACGCGCTCAAATACGCGATGCGTGAAGACCCCGACGTCATCCTCGTCGGGGAGATGCGTGACAAGGAGA
CCATCGCCGCAGCACTCACCGCCGCCGAGACCGGTCACCTCGTTTTCGGTACCCTGCACACCAACTCCGCGCCGCAGACG
ATCAACCGTATCATCGACGTCTTCAGCGGGGACGAGCAGCCGCAGGTCCGCGCCCAGCTCTCCACGTCCATCATCGCCAT
TATCGCCCAGGCGCTGCTGCCGCGCCTCGGCGGCGGCCGTGTCGCAGCCCAGGAGCTGATGATCAACATCCCGGCGATCG
CCAACCTGATCCGCGAAGACAAGGTCCACCAGCTCTACTCACAGATGCAGCTCAACCAGCAGGAGACCGGCATGGTCACC
CAGACCCAGGAACTCTTCGACCTGGTACGCAAACGCGTCATCTCCAAGCAAGACGCCATCCAGTGGTCCAACCGCCCCGA
CGAACTCATCAAGGCGCTTGACGCCGCCGCGCTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-274)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

51.734

96.648

0.5
  pilT Acinetobacter baumannii D1279779

51.734

96.648

0.5
  pilT Acinetobacter baumannii strain A118

51.734

96.648

0.5
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

52.994

93.296

0.494
  pilT Vibrio cholerae strain A1552

52.994

93.296

0.494
  pilT Legionella pneumophila strain ERS1305867

52.537

93.575

0.492
  pilT Legionella pneumophila strain Lp02

52.537

93.575

0.492
  pilT Pseudomonas stutzeri DSM 10701

50.435

96.369

0.486
  pilT Acinetobacter baylyi ADP1

50.145

96.369

0.483
  pilT Pseudomonas aeruginosa PAK

49.565

96.369

0.478
  pilT Neisseria gonorrhoeae MS11

49.702

93.855

0.466
  pilT Neisseria meningitidis 8013

49.405

93.855

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.733

100

0.439
  pilU Acinetobacter baylyi ADP1

40.346

96.927

0.391
  pilU Pseudomonas stutzeri DSM 10701

38.439

96.648

0.372