Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   V9L13_RS16320 Genome accession   NZ_CP146986
Coordinates   3623876..3624316 (-) Length   146 a.a.
NCBI ID   WP_338800026.1    Uniprot ID   -
Organism   Pseudomonas sp. RSB 5.4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3618876..3629316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9L13_RS16305 (V9L13_16305) - 3619698..3622655 (+) 2958 WP_338800024.1 DUF748 domain-containing protein -
  V9L13_RS16310 (V9L13_16310) - 3622655..3622951 (+) 297 WP_338800025.1 DUF2845 domain-containing protein -
  V9L13_RS16315 (V9L13_16315) - 3623221..3623577 (-) 357 WP_025108814.1 BON domain-containing protein -
  V9L13_RS16320 (V9L13_16320) pilA 3623876..3624316 (-) 441 WP_338800026.1 pilin Machinery gene
  V9L13_RS16325 (V9L13_16325) pilB 3624545..3626245 (+) 1701 WP_338800027.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V9L13_RS16330 (V9L13_16330) pilC 3626248..3627465 (+) 1218 WP_338800028.1 type II secretion system F family protein Machinery gene
  V9L13_RS16335 (V9L13_16335) pilD 3627467..3628339 (+) 873 WP_338800029.1 A24 family peptidase Machinery gene
  V9L13_RS16340 (V9L13_16340) coaE 3628336..3628959 (+) 624 WP_338800030.1 dephospho-CoA kinase -
  V9L13_RS16345 (V9L13_16345) yacG 3628956..3629153 (+) 198 WP_003228296.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 146 a.a.        Molecular weight: 14560.69 Da        Isoelectric Point: 8.1087

>NTDB_id=945372 V9L13_RS16320 WP_338800026.1 3623876..3624316(-) (pilA) [Pseudomonas sp. RSB 5.4]
MNNQKGFTLIELLIVVAIIGILATIALPQYSKYQARSKVTAGLAEISALKVPFEDTINQGTDPTLLLVAGSATATTSNCT
LAASGTATTGAGTITCTLVNAPGPVLGKTITLTRTGAATGNTSGVWSCASTVNPDYAPKGCAGTGA

Nucleotide


Download         Length: 441 bp        

>NTDB_id=945372 V9L13_RS16320 WP_338800026.1 3623876..3624316(-) (pilA) [Pseudomonas sp. RSB 5.4]
ATGAACAACCAAAAAGGTTTCACTCTGATCGAGCTGCTGATCGTGGTGGCGATCATCGGCATTCTGGCGACCATCGCCTT
GCCGCAATACTCCAAGTATCAGGCGCGTTCGAAGGTGACGGCAGGTTTGGCCGAAATCAGCGCGCTGAAGGTGCCGTTCG
AGGACACCATTAATCAAGGTACCGATCCAACCCTGTTACTTGTGGCCGGTAGTGCCACAGCGACCACCTCCAACTGCACA
CTGGCGGCTTCGGGAACAGCTACGACAGGGGCCGGCACCATCACCTGCACACTGGTGAACGCCCCGGGCCCGGTGCTGGG
TAAAACCATCACCCTTACCCGCACGGGGGCTGCAACCGGCAACACCTCCGGCGTCTGGAGCTGTGCATCGACCGTCAACC
CGGACTACGCGCCTAAAGGTTGCGCGGGTACCGGTGCCTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(35-141)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

50.34

100

0.507
  pilA/pilAI Pseudomonas stutzeri DSM 10701

46.429

95.89

0.445
  pilA Pseudomonas aeruginosa PAK

42.208

100

0.445
  comP Acinetobacter baylyi ADP1

41.026

100

0.438
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.39

96.575

0.438
  pilA Vibrio cholerae strain A1552

42

100

0.432
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

42

100

0.432
  pilA Vibrio cholerae C6706

42

100

0.432
  pilA Ralstonia pseudosolanacearum GMI1000

38.272

100

0.425
  pilA2 Legionella pneumophila strain ERS1305867

40.845

97.26

0.397
  pilA2 Legionella pneumophila str. Paris

40.845

97.26

0.397
  pilA Vibrio parahaemolyticus RIMD 2210633

39.716

96.575

0.384