Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   V9L13_RS16325 Genome accession   NZ_CP146986
Coordinates   3624545..3626245 (+) Length   566 a.a.
NCBI ID   WP_338800027.1    Uniprot ID   -
Organism   Pseudomonas sp. RSB 5.4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3619545..3631245
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V9L13_RS16305 (V9L13_16305) - 3619698..3622655 (+) 2958 WP_338800024.1 DUF748 domain-containing protein -
  V9L13_RS16310 (V9L13_16310) - 3622655..3622951 (+) 297 WP_338800025.1 DUF2845 domain-containing protein -
  V9L13_RS16315 (V9L13_16315) - 3623221..3623577 (-) 357 WP_025108814.1 BON domain-containing protein -
  V9L13_RS16320 (V9L13_16320) pilA 3623876..3624316 (-) 441 WP_338800026.1 pilin Machinery gene
  V9L13_RS16325 (V9L13_16325) pilB 3624545..3626245 (+) 1701 WP_338800027.1 type IV-A pilus assembly ATPase PilB Machinery gene
  V9L13_RS16330 (V9L13_16330) pilC 3626248..3627465 (+) 1218 WP_338800028.1 type II secretion system F family protein Machinery gene
  V9L13_RS16335 (V9L13_16335) pilD 3627467..3628339 (+) 873 WP_338800029.1 A24 family peptidase Machinery gene
  V9L13_RS16340 (V9L13_16340) coaE 3628336..3628959 (+) 624 WP_338800030.1 dephospho-CoA kinase -
  V9L13_RS16345 (V9L13_16345) yacG 3628956..3629153 (+) 198 WP_003228296.1 DNA gyrase inhibitor YacG -
  V9L13_RS16350 (V9L13_16350) - 3629156..3629371 (-) 216 WP_003228298.1 hypothetical protein -
  V9L13_RS16355 (V9L13_16355) - 3629437..3630126 (-) 690 WP_338800031.1 energy-coupling factor ABC transporter permease -
  V9L13_RS16360 (V9L13_16360) - 3630245..3630871 (+) 627 WP_003228301.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62491.51 Da        Isoelectric Point: 5.9749

>NTDB_id=945373 V9L13_RS16325 WP_338800027.1 3624545..3626245(+) (pilB) [Pseudomonas sp. RSB 5.4]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSSQVAEIASEHFGMAFMDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTEAIEKFFDTHASGLEEMADVDLDG
VDVESVDDSRQDAIGGLDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNFRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKTKSIDFRVNTLPTLWGEKVVIRILDPSSAQIGINALGYEPAQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPP
EQIGSFTIYEPVGCDLCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDSQMRRDGFDDLRTSGLHKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=945373 V9L13_RS16325 WP_338800027.1 3624545..3626245(+) (pilB) [Pseudomonas sp. RSB 5.4]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAGCAATTGGTACAGGCCGAACTGCTTACGGAAAAAAGCGCCCAGCAGGC
CTGGCAACAGGCGCAGCGCAATCGGCTGTCGCTGGTCAGCTACCTGGTGCAGAACAAACTGGTGAAAAGCTCACAGGTCG
CCGAGATCGCTTCCGAGCATTTCGGCATGGCCTTCATGGACCTCAATTGCCTGGACAAGGAAACCCAGCCCAAAGGCCTG
GTCAGTGAAAAACTGGTGCGCCAGCATCACGCCCTGCCCTTGTGGCGGCGTGGCAACAAACTGTTCGTGGGCATTTCCGA
CCCGAGCAATCATCAGGCGATCAATGACATTCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTGGAGGACGACA
AGCTCACCGAGGCCATCGAGAAATTCTTCGACACCCACGCCAGCGGCCTCGAAGAAATGGCCGACGTCGATCTCGACGGC
GTGGATGTCGAGTCCGTCGACGACAGCCGCCAGGACGCCATCGGCGGACTCGATGCCGATGACGCGCCGGTCGTGCGTTT
CGTGCACAAGATGCTGCTCGACGCGATCAAAAGTGGTTCGTCGGACCTGCACTTCGAACCCTACGAAAAAAACTTCCGGG
TGCGCGTGCGTACCGACGGCATCCTGCGCGAGGTGGCCAAACCGCCGATCCAGTTGGCCGGGCGAATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATCTCGGAGCGACGCAAACCCCAGGACGGGCGGATCAAGATGCGCCTGTCGAAGACCAA
GTCCATCGACTTCCGGGTCAACACCCTGCCGACCCTGTGGGGCGAAAAAGTGGTGATCCGGATTCTCGATCCCTCCAGTG
CGCAGATCGGCATCAATGCGTTGGGTTACGAGCCGGCGCAGAAAGACCTGTACATGGCAGCACTGAAGCAGCCGCAGGGC
ATGATTCTGGTGACCGGGCCTACCGGGTCGGGGAAAACCGTATCGCTGTACACCGGCCTGAATATTCTCAACACCGTCGA
CATCAACATTTCGACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAACCCCAAGCAAG
GACTGGATTTTGCCCAGGCCCTGCGTTCGTTTCTGCGGCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAAACCGCCGAAATCGCGATCAAGGCCGCGCAGACCGGCCACCTGGTGCTGTCCACTCTGCACACCAACAGCGCCGCCGA
AACCCTGACCCGCCTGCACAACATGGGCATTCCCGGGTTCAACATCGCCACCTCGGTGAGCCTGATCATCGCCCAGCGTC
TGGCGCGCAAGCTGTGCAGCCACTGCAAGAAACCGATCGAGATCCCCCGCGAAACGCTGATCAAGGAAGGTTTCCCGCCG
GAACAGATCGGCAGTTTCACGATCTATGAGCCGGTCGGTTGCGACCTCTGCAACGGCGGCTACAAGGGACGCGTGGGGAT
TTACGAAGTGGTGAAAAACACCCCTGACCTGCAACGGCTGATCATGGCCGAAGGCAATTCGCTGGAAATCGACAGCCAGA
TGCGTCGCGACGGCTTCGACGACCTGCGCACCTCGGGCCTGCACAAGGCCATGCAAGGCATCACCAGCCTGGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.699

99.823

0.576
  pilB Acinetobacter baumannii D1279779

57.27

99.647

0.571
  pilB Legionella pneumophila strain ERS1305867

55.477

100

0.555
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilB Vibrio campbellii strain DS40M4

49.561

100

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

49.733

99.117

0.493
  pilF Neisseria gonorrhoeae MS11

49.201

99.47

0.489
  pilF Thermus thermophilus HB27

36.738

98.587

0.362