Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   NGK25_RS18240 Genome accession   NZ_AP025797
Coordinates   3818407..3819387 (-) Length   326 a.a.
NCBI ID   WP_022649354.1    Uniprot ID   A0A145XVK6
Organism   Enterobacter hormaechei strain FUJ80022     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3813407..3824387
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGK25_RS18210 (FJMB80022_35710) - 3814284..3814781 (+) 498 WP_032621896.1 SprT family zinc-dependent metalloprotease -
  NGK25_RS18215 (FJMB80022_35720) endA 3814876..3815583 (+) 708 WP_022649352.1 deoxyribonuclease I -
  NGK25_RS18220 (FJMB80022_35730) rsmE 3815635..3816366 (+) 732 WP_003860033.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  NGK25_RS18225 (FJMB80022_35740) gshB 3816398..3817345 (+) 948 WP_003860032.1 glutathione synthase -
  NGK25_RS18230 (FJMB80022_35750) - 3817420..3817980 (+) 561 WP_022649353.1 YqgE/AlgH family protein -
  NGK25_RS18235 (FJMB80022_35760) ruvX 3817980..3818396 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  NGK25_RS18240 (FJMB80022_35770) pilT 3818407..3819387 (-) 981 WP_022649354.1 type IV pilus twitching motility protein PilT Machinery gene
  NGK25_RS18245 (FJMB80022_35780) - 3819405..3820106 (+) 702 WP_022649355.1 YggS family pyridoxal phosphate-dependent enzyme -
  NGK25_RS18250 (FJMB80022_35790) - 3820128..3820694 (+) 567 WP_003860024.1 YggT family protein -
  NGK25_RS18255 (FJMB80022_35800) yggU 3820691..3820987 (+) 297 WP_022649356.1 DUF167 family protein YggU -
  NGK25_RS18260 (FJMB80022_35810) - 3820991..3821584 (+) 594 WP_022649357.1 XTP/dITP diphosphatase -
  NGK25_RS18265 (FJMB80022_35820) hemW 3821577..3822725 (+) 1149 WP_022649358.1 radical SAM family heme chaperone HemW -
  NGK25_RS18270 (FJMB80022_35830) - 3822782..3823120 (+) 339 WP_022649359.1 endonuclease domain-containing protein -
  NGK25_RS18275 (FJMB80022_35840) - 3823204..3823920 (-) 717 WP_022649360.1 DUF2884 domain-containing protein -
  NGK25_RS18280 (FJMB80022_35850) - 3823978..3824304 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35674.64 Da        Isoelectric Point: 6.5004

>NTDB_id=94315 NGK25_RS18240 WP_022649354.1 3818407..3819387(-) (pilT) [Enterobacter hormaechei strain FUJ80022]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLTGGQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIRQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=94315 NGK25_RS18240 WP_022649354.1 3818407..3819387(-) (pilT) [Enterobacter hormaechei strain FUJ80022]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCCGATGTGGAGGCGTTATTAAAAGCGTGGCTCAACG
ACGAGCAGCAGGGTGCCTGGTGGGCAAACGGGCAGGTAGATTTTGCCGTTACCCTCACAGGCGGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATCGCGCTACGCCTGTTGCCGCTGACGTGTCCGCAGCTCTCTGCGCTAGG
CGTGCCGCGCGCGATCCCGGAGCTTTTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGGAAAT
CGACCACCCTGGCCGCGATGGTCGATTTTCTCAATCACCATACGGACGGGCACATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTCATCCGGCAGCGGGAGATAGGCCAGCATAGCCCGTCCTTTGCCGAGGCCCTGCG
CAGTGCGCTGCGTGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGG
CGGCTGAGACTGGCCATCTGGTGTTGGCCACGCTGCACACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGATACC
TTCCCGGCGCAGGAGAAAGATCCGGTTCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTACTGGCGCAGAAGCTGCGTCA
GGATGTCCAGGGCGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACGTCGGCGGCGGCAAACCTGATCCGCGAAGGCA
AAACCTGGCAACTGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGGATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGA
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(75-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A145XVK6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.765

100

0.509
  pilT Vibrio cholerae strain A1552

50.765

100

0.509
  pilT Pseudomonas stutzeri DSM 10701

48.012

100

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Legionella pneumophila strain ERS1305867

48.447

98.773

0.479
  pilT Legionella pneumophila strain Lp02

48.447

98.773

0.479
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baylyi ADP1

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

47.095

100

0.472
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.344

99.08

0.429
  pilU Pseudomonas stutzeri DSM 10701

38.182

100

0.387
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38

Multiple sequence alignment