Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PSGK_RS28935 Genome accession   NZ_CP146285
Coordinates   6382072..6382497 (-) Length   141 a.a.
NCBI ID   WP_374979915.1    Uniprot ID   -
Organism   Pseudomonas solani strain GK13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6377072..6387497
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSGK_RS28905 (PSGK_28755) - 6377115..6377330 (+) 216 WP_043244210.1 hypothetical protein -
  PSGK_RS28910 (PSGK_28760) yacG 6377334..6377534 (-) 201 WP_021221832.1 DNA gyrase inhibitor YacG -
  PSGK_RS28915 (PSGK_28765) coaE 6377531..6378142 (-) 612 WP_271660079.1 dephospho-CoA kinase -
  PSGK_RS28920 (PSGK_28770) pilD 6378142..6379011 (-) 870 WP_374979912.1 prepilin peptidase Machinery gene
  PSGK_RS28925 (PSGK_28775) pilC 6379012..6380232 (-) 1221 WP_374979913.1 type II secretion system F family protein Machinery gene
  PSGK_RS28930 (PSGK_28780) pilB 6380236..6381939 (-) 1704 WP_374979914.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSGK_RS28935 (PSGK_28785) pilA 6382072..6382497 (-) 426 WP_374979915.1 pilin Machinery gene
  PSGK_RS28940 (PSGK_28790) - 6382601..6384379 (+) 1779 WP_374979916.1 O-antigen ligase family protein -
  PSGK_RS28950 (PSGK_28800) nadC 6384557..6385405 (-) 849 WP_374979917.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 141 a.a.        Molecular weight: 14309.23 Da        Isoelectric Point: 9.1334

>NTDB_id=942513 PSGK_RS28935 WP_374979915.1 6382072..6382497(-) (pilA) [Pseudomonas solani strain GK13]
MKAQKGFTLIELMIVVAIIGILAAIAIPAYQNYIGRSEAATGLAAIAPLKTAVEDSYAQGRPDTSITIGNLGSSGSASPL
GAITVSNFSNGAGNLTFTFGSAVGGNSGPKTNGRNIVLARNASGTWSCSSSLDPEFKPRNC

Nucleotide


Download         Length: 426 bp        

>NTDB_id=942513 PSGK_RS28935 WP_374979915.1 6382072..6382497(-) (pilA) [Pseudomonas solani strain GK13]
ATGAAAGCACAAAAAGGCTTCACCCTGATCGAACTGATGATTGTCGTTGCAATCATCGGCATCCTGGCCGCCATCGCAAT
CCCCGCATACCAGAACTACATTGGCCGCTCCGAAGCGGCTACCGGTCTGGCCGCTATCGCCCCTCTGAAAACCGCTGTGG
AAGATTCCTACGCCCAAGGCCGTCCTGATACCTCCATCACCATCGGCAACCTCGGCAGCTCTGGCAGTGCCAGCCCGCTG
GGTGCCATCACAGTAAGCAACTTCTCGAACGGCGCAGGCAACCTGACCTTCACCTTTGGCTCCGCCGTCGGCGGCAACTC
TGGCCCGAAAACCAACGGCCGGAACATCGTCCTGGCCCGCAACGCCAGCGGTACCTGGTCCTGCAGCAGCTCCCTGGACC
CCGAGTTCAAGCCGCGTAACTGCTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(36-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

48.344

100

0.518
  pilA/pilAI Pseudomonas stutzeri DSM 10701

48.951

100

0.496
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

44.218

100

0.461
  pilA Vibrio cholerae strain A1552

44.218

100

0.461
  pilA Vibrio cholerae C6706

44.218

100

0.461
  pilA/pilAII Pseudomonas stutzeri DSM 10701

45.324

98.582

0.447
  pilA Acinetobacter baumannii strain A118

43.662

100

0.44
  comP Acinetobacter baylyi ADP1

40.132

100

0.433
  pilA Vibrio parahaemolyticus RIMD 2210633

46.457

90.071

0.418
  pilA2 Legionella pneumophila str. Paris

40.972

100

0.418
  pilA2 Legionella pneumophila strain ERS1305867

40.972

100

0.418
  pilA/pilA1 Eikenella corrodens VA1

40.146

97.163

0.39