Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PSGK_RS28930 Genome accession   NZ_CP146285
Coordinates   6380236..6381939 (-) Length   567 a.a.
NCBI ID   WP_374979914.1    Uniprot ID   -
Organism   Pseudomonas solani strain GK13     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6375236..6386939
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSGK_RS28890 (PSGK_28740) - 6375435..6375860 (+) 426 WP_265167702.1 GNAT family N-acetyltransferase -
  PSGK_RS28895 (PSGK_28745) - 6375857..6376324 (+) 468 WP_021221829.1 hypothetical protein -
  PSGK_RS28900 (PSGK_28750) - 6376321..6377007 (+) 687 WP_374979911.1 energy-coupling factor ABC transporter permease -
  PSGK_RS28905 (PSGK_28755) - 6377115..6377330 (+) 216 WP_043244210.1 hypothetical protein -
  PSGK_RS28910 (PSGK_28760) yacG 6377334..6377534 (-) 201 WP_021221832.1 DNA gyrase inhibitor YacG -
  PSGK_RS28915 (PSGK_28765) coaE 6377531..6378142 (-) 612 WP_271660079.1 dephospho-CoA kinase -
  PSGK_RS28920 (PSGK_28770) pilD 6378142..6379011 (-) 870 WP_374979912.1 prepilin peptidase Machinery gene
  PSGK_RS28925 (PSGK_28775) pilC 6379012..6380232 (-) 1221 WP_374979913.1 type II secretion system F family protein Machinery gene
  PSGK_RS28930 (PSGK_28780) pilB 6380236..6381939 (-) 1704 WP_374979914.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSGK_RS28935 (PSGK_28785) pilA 6382072..6382497 (-) 426 WP_374979915.1 pilin Machinery gene
  PSGK_RS28940 (PSGK_28790) - 6382601..6384379 (+) 1779 WP_374979916.1 O-antigen ligase family protein -
  PSGK_RS28950 (PSGK_28800) nadC 6384557..6385405 (-) 849 WP_374979917.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62449.45 Da        Isoelectric Point: 5.4723

>NTDB_id=942512 PSGK_RS28930 WP_374979914.1 6380236..6381939(-) (pilB) [Pseudomonas solani strain GK13]
MNDSINLSGLAKQMVLAELMDEKSAHQAQLQAQRNKLSLVTYLVQNKLVKSRALAELSAEQFGVAFFDLSAIDKEGQPKE
LISEKLIRQHRVIPLWRRGNKLFVGVSDPTNHQAVTDIQFSTGLTTEAILVEDDKLGDAIEKFFDSANSGMEDLGDVDLD
GVDIEAVDDDKQDSGAGESADDAPVVRFVNKMLLDAIKGGSSDLHFEPYERSYRVRLRTDGILHEVARPPIQLASRISAR
LKVMAALDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEESQKELYLNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCNTCKKEIEVPRDTLLKEGFP
EDKIGAFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDIAAQMRKDGFNDLRNSGLAKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=942512 PSGK_RS28930 WP_374979914.1 6380236..6381939(-) (pilB) [Pseudomonas solani strain GK13]
ATGAATGACAGCATAAATCTTTCCGGCTTGGCCAAGCAGATGGTTCTCGCGGAGCTGATGGACGAGAAAAGTGCTCATCA
AGCACAACTCCAGGCCCAACGCAACAAACTCTCACTGGTCACGTACCTGGTACAGAACAAGCTGGTTAAAAGCCGGGCAC
TGGCAGAACTATCTGCAGAGCAGTTCGGTGTGGCGTTCTTCGACCTGAGCGCAATTGACAAGGAAGGACAGCCTAAAGAG
CTAATCAGCGAAAAACTGATTCGCCAACACCGTGTGATACCGCTCTGGCGCAGGGGAAACAAGCTCTTCGTTGGGGTTTC
CGACCCCACCAATCACCAAGCCGTGACCGATATCCAGTTCAGCACGGGTCTCACGACTGAAGCGATTCTTGTCGAAGACG
ACAAGCTCGGCGATGCCATCGAAAAATTCTTCGACAGTGCCAACTCCGGAATGGAAGACCTTGGTGATGTCGATCTGGAC
GGCGTTGATATCGAGGCGGTCGACGACGACAAGCAAGACAGCGGAGCAGGTGAAAGCGCTGATGACGCCCCCGTCGTACG
CTTCGTCAACAAGATGCTGCTGGATGCCATCAAGGGGGGGTCCTCCGACCTTCACTTTGAGCCCTATGAGCGCTCATACC
GCGTCCGGCTACGCACCGATGGCATTCTCCACGAAGTAGCGCGCCCACCGATCCAACTGGCCAGCCGCATTTCCGCACGC
TTGAAGGTCATGGCAGCCCTTGATATTTCCGAACGTCGCAAGCCTCAGGACGGCCGGATCAAGATGAAGATATCCAAGAC
CAAAGCCATCGACTTCCGTGTCAACACGCTCCCCACCCTTTGGGGCGAGAAGATCGTGATGCGGATCCTTGACCCATCCA
GCGCACAGATGGGTATCGACGCACTGGGTTACGAAGAGTCTCAGAAGGAGCTCTATCTAAACGCGTTGAAGCAACCTCAA
GGCATGATCCTTGTGACGGGTCCCACAGGCTCGGGTAAAACGGTTTCGCTCTACACGGGCCTGAACATCCTGAACACGGT
CGATATCAACATTTCCACCGCTGAAGACCCGGTAGAAATCAACCTCGAAGGGATCAATCAGGTCAACGTCAATCCACGCC
AAGGCATGGACTTCGCCCAAGCTCTACGTGCCTTCCTTCGACAGGACCCGGACGTGATCATGGTCGGCGAGATCCGGGAC
CTGGAAACCGCAGAGATCGCGATCAAAGCAGCCCAGACCGGCCATATGGTGATGTCCACCCTGCACACCAACAGCGCGGC
GGAAACCTTGACACGCTTGCGCAACATGGGCGTTCCAGCGTTCAACATCGCAACCTCAGTCAATCTGATCATCGCCCAGC
GCCTTGCTCGAAAGCTTTGCAACACCTGCAAAAAGGAGATCGAGGTTCCCCGAGACACCCTTCTCAAGGAAGGTTTCCCC
GAAGACAAGATTGGTGCCTTCAAGATTTACGGCCCCGTAGGTTGCGATAACTGCAAAGGCGGTTACAAAGGCAGGGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCCCTGCAGCGGATTATCATGGAGGAAGGCAACTCCATCGATATCGCTGCGC
AAATGCGTAAAGATGGCTTCAATGATCTGCGCAACTCGGGCCTGGCAAAAGCTATGCAAGGTGTGACCAGCCTCGAGGAA
GTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.605

98.236

0.586
  pilB Acinetobacter baylyi ADP1

58.615

99.295

0.582
  pilB Legionella pneumophila strain ERS1305867

55.556

100

0.556
  pilB Vibrio cholerae strain A1552

50.802

98.942

0.503
  pilB Vibrio parahaemolyticus RIMD 2210633

50.177

99.471

0.499
  pilB Vibrio campbellii strain DS40M4

49.293

99.824

0.492
  pilF Neisseria gonorrhoeae MS11

48.845

99.295

0.485
  pilB/pilB1 Synechocystis sp. PCC 6803

35.266

100

0.386
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.076

93.298

0.374
  pilF Thermus thermophilus HB27

36.46

99.647

0.363