Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PSGK_RS28925 Genome accession   NZ_CP146285
Coordinates   6379012..6380232 (-) Length   406 a.a.
NCBI ID   WP_374979913.1    Uniprot ID   -
Organism   Pseudomonas solani strain GK13     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6374012..6385232
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSGK_RS28880 (PSGK_28730) - 6374435..6374608 (-) 174 WP_021221826.1 DUF3094 family protein -
  PSGK_RS28885 (PSGK_28735) - 6374695..6375321 (-) 627 WP_021221827.1 DUF1780 domain-containing protein -
  PSGK_RS28890 (PSGK_28740) - 6375435..6375860 (+) 426 WP_265167702.1 GNAT family N-acetyltransferase -
  PSGK_RS28895 (PSGK_28745) - 6375857..6376324 (+) 468 WP_021221829.1 hypothetical protein -
  PSGK_RS28900 (PSGK_28750) - 6376321..6377007 (+) 687 WP_374979911.1 energy-coupling factor ABC transporter permease -
  PSGK_RS28905 (PSGK_28755) - 6377115..6377330 (+) 216 WP_043244210.1 hypothetical protein -
  PSGK_RS28910 (PSGK_28760) yacG 6377334..6377534 (-) 201 WP_021221832.1 DNA gyrase inhibitor YacG -
  PSGK_RS28915 (PSGK_28765) coaE 6377531..6378142 (-) 612 WP_271660079.1 dephospho-CoA kinase -
  PSGK_RS28920 (PSGK_28770) pilD 6378142..6379011 (-) 870 WP_374979912.1 prepilin peptidase Machinery gene
  PSGK_RS28925 (PSGK_28775) pilC 6379012..6380232 (-) 1221 WP_374979913.1 type II secretion system F family protein Machinery gene
  PSGK_RS28930 (PSGK_28780) pilB 6380236..6381939 (-) 1704 WP_374979914.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PSGK_RS28935 (PSGK_28785) pilA 6382072..6382497 (-) 426 WP_374979915.1 pilin Machinery gene
  PSGK_RS28940 (PSGK_28790) - 6382601..6384379 (+) 1779 WP_374979916.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44015.81 Da        Isoelectric Point: 9.8620

>NTDB_id=942511 PSGK_RS28925 WP_374979913.1 6379012..6380232(-) (pilC) [Pseudomonas solani strain GK13]
MAEKALKASVFKWEGTDKKGTKVTGEISGINPALVKAQLRKQGINPLKVRKKAASIFGAGKKVKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPNMRKLVDEVKQEVAAGNSFANSLRKKPMYFDDLYCNLVDSGEQSGALETLLDRVATYKEKT
EQLKAKIKKAMTYPIAVLIVAVIVSAILLIKVVPQFQDVFKGFGAELPAFTQMVVNLSEALQTWGFLILIATFGIIFGIG
QFYKRSQKFRDAVDKGVLKMPIVGDIVYKSSVARYARTLSTTFAAGVPLVDALDSVSGATGNVVFKTAVNKIKQDVSTGM
QLNFSMRTTGVFPSMAIQMTAIGEESGALDTMLDKVASFYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFT
LGSVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=942511 PSGK_RS28925 WP_374979913.1 6379012..6380232(-) (pilC) [Pseudomonas solani strain GK13]
ATGGCGGAAAAAGCATTAAAAGCGAGTGTCTTCAAATGGGAAGGCACCGACAAGAAAGGTACCAAGGTCACTGGTGAGAT
CAGCGGCATAAACCCGGCGCTGGTGAAAGCACAGCTCCGCAAGCAGGGTATCAACCCGCTCAAGGTACGCAAAAAGGCAG
CCTCGATTTTCGGAGCGGGCAAGAAGGTCAAGCCGCTGGATATCGCGTTGTTTACCCGCCAGATGGCCACGATGATGAAG
GCTGGCGTTCCGTTGCTGCAAAGCTTCGACATCATTGGTGAAGGCTTCGAAAACCCCAACATGCGCAAGCTGGTGGACGA
GGTGAAGCAGGAAGTGGCAGCAGGCAACAGCTTCGCCAACTCTCTGCGCAAGAAACCCATGTATTTCGATGACCTGTATT
GCAACCTGGTGGACTCTGGCGAGCAATCCGGTGCGTTGGAGACATTGCTGGACCGTGTCGCAACCTACAAGGAAAAGACG
GAGCAACTGAAGGCCAAGATCAAAAAGGCCATGACCTACCCGATTGCAGTTCTGATCGTAGCAGTCATCGTGTCAGCTAT
CCTACTGATCAAGGTCGTGCCACAGTTCCAAGACGTTTTCAAAGGTTTTGGCGCAGAGCTGCCTGCATTTACCCAAATGG
TCGTGAACCTTTCCGAAGCGCTTCAAACTTGGGGATTCCTCATACTAATCGCCACATTCGGAATCATTTTTGGTATCGGC
CAATTCTACAAGCGCTCTCAGAAATTCCGAGACGCTGTAGATAAGGGTGTACTAAAAATGCCTATCGTGGGTGATATCGT
CTACAAGTCATCTGTAGCTCGTTATGCACGCACGCTGTCAACCACATTCGCAGCCGGCGTCCCACTGGTAGACGCTCTGG
ACTCTGTATCTGGAGCCACCGGCAACGTTGTATTCAAAACTGCGGTTAATAAGATCAAGCAGGATGTCTCCACCGGTATG
CAACTGAATTTCTCCATGCGTACTACCGGTGTATTCCCGTCAATGGCCATCCAGATGACGGCCATCGGTGAGGAGTCCGG
TGCGCTCGACACGATGCTGGACAAGGTCGCCTCCTTCTACGAAGAAGAAGTCGACAACGCCGTAGACAACCTGACCACCC
TGATGGAGCCCATGATCATGGCCGTCCTCGGCGTACTGGTCGGCGGCCTGATCATCGCGATGTACCTGCCTATCTTCACG
CTCGGTTCGGTAGTGGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.753

99.754

0.796
  pilC Acinetobacter baylyi ADP1

61.33

100

0.613
  pilC Acinetobacter baumannii D1279779

61.481

99.754

0.613
  pilC Legionella pneumophila strain ERS1305867

52.525

97.537

0.512
  pilG Neisseria gonorrhoeae MS11

44.776

99.015

0.443
  pilG Neisseria meningitidis 44/76-A

44.417

99.261

0.441
  pilC Vibrio cholerae strain A1552

42.857

100

0.429
  pilC Vibrio campbellii strain DS40M4

42.065

97.783

0.411
  pilC Thermus thermophilus HB27

36.725

99.261

0.365