Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   NUH87_RS21735 Genome accession   NZ_CP144470
Coordinates   4760524..4760925 (+) Length   133 a.a.
NCBI ID   WP_338522623.1    Uniprot ID   -
Organism   Pseudomonas batumici strain Koz11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4755524..4765925
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUH87_RS21710 (NUH87_21710) yacG 4755680..4755883 (-) 204 WP_040071046.1 DNA gyrase inhibitor YacG -
  NUH87_RS21715 (NUH87_21715) coaE 4755880..4756503 (-) 624 WP_338522619.1 dephospho-CoA kinase -
  NUH87_RS21720 (NUH87_21720) pilD 4756500..4757372 (-) 873 WP_338522620.1 A24 family peptidase Machinery gene
  NUH87_RS21725 (NUH87_21725) pilC 4757376..4758593 (-) 1218 WP_338522621.1 type II secretion system F family protein Machinery gene
  NUH87_RS21730 (NUH87_21730) pilB 4758597..4760297 (-) 1701 WP_338522622.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NUH87_RS21735 (NUH87_21735) pilA2 4760524..4760925 (+) 402 WP_338522623.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NUH87_RS21740 (NUH87_21740) - 4761046..4762692 (+) 1647 WP_338522625.1 hypothetical protein -
  NUH87_RS21745 (NUH87_21745) - 4763097..4764152 (+) 1056 WP_338522626.1 O-antigen ligase family protein -
  NUH87_RS21755 (NUH87_21755) - 4764327..4765823 (+) 1497 WP_338522627.1 AlkA N-terminal domain-containing protein -

Sequence

Protein


Download         Length: 133 a.a.        Molecular weight: 13438.45 Da        Isoelectric Point: 6.8894

>NTDB_id=934003 NUH87_RS21735 WP_338522623.1 4760524..4760925(+) (pilA2) [Pseudomonas batumici strain Koz11]
MNAQKGFTLIELMIVVAIIGILAAIALPAYNTYTQKAHFAEVISIADGYKSAVAVCMVENANSSGSLCGAGSNGVPAVMV
STSVSSMTVANGLISMTATNAAGGYTYTLQANTSGNAITWTVGGTCLNAGFCR

Nucleotide


Download         Length: 402 bp        

>NTDB_id=934003 NUH87_RS21735 WP_338522623.1 4760524..4760925(+) (pilA2) [Pseudomonas batumici strain Koz11]
ATGAATGCTCAAAAGGGTTTCACCTTGATCGAACTGATGATCGTTGTGGCGATCATCGGCATTTTGGCGGCGATTGCGTT
GCCTGCGTATAACACCTATACACAAAAGGCCCACTTTGCCGAGGTCATTTCGATTGCGGATGGATACAAGAGTGCTGTGG
CAGTTTGCATGGTAGAAAACGCAAACTCGAGCGGCAGTCTGTGTGGTGCGGGCTCAAATGGTGTGCCGGCAGTAATGGTG
TCGACCAGCGTGTCTTCGATGACGGTCGCGAATGGGCTTATCTCAATGACCGCGACAAATGCTGCAGGCGGTTACACCTA
CACGCTTCAGGCTAACACGTCAGGTAATGCGATTACGTGGACTGTTGGTGGGACCTGCTTGAACGCTGGTTTCTGCAGAT
AA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

45.27

100

0.504
  pilA2 Legionella pneumophila str. Paris

45.27

100

0.504
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.727

100

0.436
  pilA Haemophilus influenzae Rd KW20

40.426

100

0.429
  pilA Ralstonia pseudosolanacearum GMI1000

46.281

90.977

0.421
  pilA Haemophilus influenzae 86-028NP

39.716

100

0.421
  comP Acinetobacter baylyi ADP1

41.481

100

0.421
  pilA Vibrio campbellii strain DS40M4

41.353

100

0.414
  pilA Glaesserella parasuis strain SC1401

44.167

90.226

0.398
  pilA Acinetobacter baumannii strain A118

43.966

87.218

0.383
  pilE Neisseria gonorrhoeae MS11

37.405

98.496

0.368
  pilA Pseudomonas aeruginosa PAK

38.889

94.737

0.368