Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NUH87_RS21730 Genome accession   NZ_CP144470
Coordinates   4758597..4760297 (-) Length   566 a.a.
NCBI ID   WP_338522622.1    Uniprot ID   -
Organism   Pseudomonas batumici strain Koz11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4753597..4765297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NUH87_RS21690 (NUH87_21690) - 4753682..4753855 (-) 174 WP_338522616.1 DUF3094 family protein -
  NUH87_RS21695 (NUH87_21695) - 4753923..4754549 (-) 627 WP_040071051.1 DUF1780 domain-containing protein -
  NUH87_RS21700 (NUH87_21700) - 4754704..4755390 (+) 687 WP_338522617.1 energy-coupling factor ABC transporter permease -
  NUH87_RS21705 (NUH87_21705) - 4755453..4755668 (+) 216 WP_338522618.1 hypothetical protein -
  NUH87_RS21710 (NUH87_21710) yacG 4755680..4755883 (-) 204 WP_040071046.1 DNA gyrase inhibitor YacG -
  NUH87_RS21715 (NUH87_21715) coaE 4755880..4756503 (-) 624 WP_338522619.1 dephospho-CoA kinase -
  NUH87_RS21720 (NUH87_21720) pilD 4756500..4757372 (-) 873 WP_338522620.1 A24 family peptidase Machinery gene
  NUH87_RS21725 (NUH87_21725) pilC 4757376..4758593 (-) 1218 WP_338522621.1 type II secretion system F family protein Machinery gene
  NUH87_RS21730 (NUH87_21730) pilB 4758597..4760297 (-) 1701 WP_338522622.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NUH87_RS21735 (NUH87_21735) pilA2 4760524..4760925 (+) 402 WP_338522623.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  NUH87_RS21740 (NUH87_21740) - 4761046..4762692 (+) 1647 WP_338522625.1 hypothetical protein -
  NUH87_RS21745 (NUH87_21745) - 4763097..4764152 (+) 1056 WP_338522626.1 O-antigen ligase family protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62687.85 Da        Isoelectric Point: 5.8887

>NTDB_id=934002 NUH87_RS21730 WP_338522622.1 4758597..4760297(-) (pilB) [Pseudomonas batumici strain Koz11]
MNDIALSGLAKQLVAAELITDKNAQQAYQQAQRNRVSLVSYLVQNKLVKSRQVAEIASEHFGVALLDLNCLDKESQPKGL
VSEKLIRQHHVLPLWRRGNKLFVGISDPSNHQAITDIQFSTGLTTEAILVEDDKLTDAIDKFFDSNSTGFEEMGDVDLEG
LDIESVDDKKQEQIAGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKVYRVRLRTDGILREVAKPPIHLATRIAARL
KVMASLDISERRKPQDGRIKMRISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLHNMGIPGFNIATSVNLIIAQRLARKLCTYCKKEIDVPEEALIKEGFPP
EKIGTFKIYEPVGCEQCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSIEIATQMRKDGFNDLRTSGLQKAMHGLTSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=934002 NUH87_RS21730 WP_338522622.1 4758597..4760297(-) (pilB) [Pseudomonas batumici strain Koz11]
ATGAACGATATCGCCCTTTCCGGTCTGGCCAAGCAACTGGTTGCCGCCGAGCTGATTACCGACAAGAACGCTCAGCAGGC
GTATCAACAGGCCCAGCGCAATCGGGTGTCCCTCGTCAGCTATCTGGTCCAGAACAAACTGGTCAAAAGCCGCCAGGTCG
CGGAAATCGCCTCGGAGCATTTCGGTGTTGCCCTGCTCGACCTGAACTGTCTCGACAAGGAAAGCCAGCCCAAAGGCCTG
GTCAGCGAGAAGCTGATCCGCCAGCACCATGTCCTGCCACTCTGGCGACGCGGCAATAAGTTGTTCGTCGGCATATCCGA
TCCTAGCAATCATCAAGCGATTACCGATATCCAGTTCAGCACCGGTCTCACGACCGAGGCCATTCTGGTAGAGGACGACA
AGCTCACCGACGCAATCGACAAGTTTTTCGACAGCAACTCCACCGGTTTTGAAGAGATGGGGGACGTCGACCTTGAAGGT
TTGGATATCGAATCGGTCGACGACAAGAAGCAGGAACAGATTGCCGGCCAGGATGCCGACGATGCCCCGGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGACGCCATCAAGGGTGGCTCCTCGGACTTGCACTTCGAGCCCTATGAAAAGGTCTACCGCG
TTCGCCTGCGCACCGACGGCATCCTGCGCGAAGTCGCCAAACCGCCGATCCATCTCGCCACCCGCATCGCCGCACGCCTG
AAAGTCATGGCCAGCCTGGATATCTCCGAACGCCGCAAACCCCAGGATGGCCGGATCAAAATGCGCATCTCGAAGACCAA
AGCCATCGACTTCCGGGTAAACACCCTGCCGACGCTGTGGGGCGAAAAGATCGTGATGCGGATCCTCGACCCGTCCAGTG
CCCAGATGGGTATTGATGCGCTCGGATACGAACCGGATCAGAAAGACCTCTATATGGCGGCCCTGAAACAACCGCAGGGC
ATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAAACCGTTTCGCTCTACACCGGCCTGAATATCCTCAATACCGTGGA
CATCAATATCTCCACCGCCGAAGACCCGGTGGAAATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCCAAACAGG
GCCTGGACTTCGCCCAGGCCCTGCGCTCGTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGAGAGATCCGCGACCTG
GAAACCGCCGAGATCGCCATCAAGGCCGCCCAGACCGGCCACATGGTCCTGTCCACCCTGCATACCAACAGCGCGGCGGA
AACCCTGACGCGCCTGCACAACATGGGCATTCCCGGTTTCAACATCGCCACCTCGGTGAACCTGATCATCGCCCAGCGAC
TGGCACGCAAACTCTGTACCTACTGCAAGAAGGAAATCGATGTCCCCGAGGAGGCACTGATCAAGGAAGGTTTCCCGCCG
GAGAAAATCGGCACCTTCAAGATCTACGAACCCGTTGGTTGTGAGCAATGCAACGGCGGCTACAAAGGTCGGGTCGGGAT
TTACGAAGTGGTCAAGAACACCCCGGACCTGCAACGGTTGATCATGGCCGAAGGCAACTCCATCGAGATCGCCACCCAGA
TGCGCAAGGACGGCTTCAATGACCTACGCACCTCGGGCCTGCAAAAAGCCATGCACGGGCTGACCAGCCTGGAAGAAGTC
AATCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.657

100

0.587
  pilB Acinetobacter baumannii D1279779

58.407

99.823

0.583
  pilB Legionella pneumophila strain ERS1305867

56.814

99.823

0.567
  pilB Vibrio cholerae strain A1552

51.418

99.647

0.512
  pilB Vibrio parahaemolyticus RIMD 2210633

48.951

100

0.495
  pilB Vibrio campbellii strain DS40M4

48.947

100

0.493
  pilF Neisseria gonorrhoeae MS11

48.845

99.47

0.486
  pilF Thermus thermophilus HB27

36.673

98.763

0.362
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.679

93.64

0.362