Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1579_RS22400 Genome accession   NZ_CP143766
Coordinates   4707501..4708481 (-) Length   326 a.a.
NCBI ID   WP_395302176.1    Uniprot ID   -
Organism   Enterobacter mori strain ECC-007     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4702501..4713481
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1579_RS22370 (V1579_22340) - 4703390..4703887 (+) 498 WP_010435446.1 SprT family zinc-dependent metalloprotease -
  V1579_RS22375 (V1579_22345) endA 4703982..4704689 (+) 708 WP_218308353.1 deoxyribonuclease I -
  V1579_RS22380 (V1579_22350) rsmE 4704741..4705472 (+) 732 WP_395302705.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  V1579_RS22385 (V1579_22355) gshB 4705492..4706439 (+) 948 WP_010435454.1 glutathione synthase -
  V1579_RS22390 (V1579_22360) - 4706514..4707074 (+) 561 WP_010435456.1 YqgE/AlgH family protein -
  V1579_RS22395 (V1579_22365) ruvX 4707074..4707490 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  V1579_RS22400 (V1579_22370) pilT 4707501..4708481 (-) 981 WP_395302176.1 type IV pilus twitching motility protein PilT Machinery gene
  V1579_RS22405 (V1579_22375) - 4708499..4709200 (+) 702 WP_281959811.1 YggS family pyridoxal phosphate-dependent enzyme -
  V1579_RS22410 (V1579_22380) - 4709222..4709788 (+) 567 WP_010435467.1 YggT family protein -
  V1579_RS22415 (V1579_22385) yggU 4709785..4710081 (+) 297 WP_042714751.1 DUF167 family protein YggU -
  V1579_RS22420 (V1579_22390) - 4710085..4710678 (+) 594 WP_214598157.1 XTP/dITP diphosphatase -
  V1579_RS22425 (V1579_22395) hemW 4710671..4711813 (+) 1143 WP_395302177.1 radical SAM family heme chaperone HemW -
  V1579_RS22430 (V1579_22400) - 4711877..4712215 (+) 339 WP_395302178.1 endonuclease domain-containing protein -
  V1579_RS22435 (V1579_22405) - 4712297..4713013 (-) 717 WP_042714747.1 DUF2884 domain-containing protein -
  V1579_RS22440 (V1579_22410) - 4713071..4713397 (-) 327 WP_010435485.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35588.58 Da        Isoelectric Point: 6.0390

>NTDB_id=931296 V1579_RS22400 WP_395302176.1 4707501..4708481(-) (pilT) [Enterobacter mori strain ECC-007]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPAPDVEALLKTWLNDEQQGAWWANGQVDFAVTLTGNQRLRG
SAFTHMKGVSIALRLLPLACPQLSALGVPRAIPELLSSDSGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSLSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLQDVQGGRVGLYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=931296 V1579_RS22400 WP_395302176.1 4707501..4708481(-) (pilT) [Enterobacter mori strain ECC-007]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCGCCGCG
CTGGCGCAGATTAGGCCGCCTTGAACCGGCCCCGTTTCCGGCCCCGGACGTTGAGGCGCTATTAAAAACCTGGCTCAACG
ATGAACAGCAGGGCGCGTGGTGGGCAAACGGACAGGTAGACTTTGCCGTCACGCTCACAGGCAATCAGCGTCTTCGCGGC
AGTGCGTTTACGCATATGAAGGGGGTGTCGATCGCCTTGCGCCTGTTGCCGCTCGCGTGCCCGCAGCTTTCTGCACTGGG
TGTGCCCAGGGCTATCCCGGAACTGCTCTCCAGTGACAGCGGGTTGATTCTGGTGACGGGTGCGACCGGTAGCGGAAAAT
CCACGACGCTCGCGGCGATGGTGGATTTTCTCAACCATCACACTGACGGCCATATTCTGACGCTCGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAGCGCTGCCTGATACAGCAGCGGGAGATTGGTCAGCACAGTCTCTCCTTTGCCGAGGCGCTGCG
TAGTGCGCTGCGCGAAGATCCGGACGTTATTCTGTTGGGAGAGCTGCGCGACAGCGAAACCATCCGCCTTGCGCTGACGG
CGGCCGAAACCGGGCACCTGGTGCTGGCGACGCTGCATACGCGCGGCGCTTCGCAGGCGATTGAACGGCTGGTGGATACC
TTCCCGGCGCAGGAGAAAGACCCGGTGCGTAACCAGCTGGCTGGCAGCCTTCGCGCTGTACTGGCGCAAAAACTGCTCCA
GGACGTGCAGGGCGGGCGCGTGGGGCTTTATGAACTGCTGGTGAACACCTCGGCGGCGGCGAATCTCATCCGCGAAGGGA
AAACGTGGCAATTACCCGGCATCATACAAACCGGTCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTCGCGGAAAGG
CGGGCGCAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Legionella pneumophila strain ERS1305867

48.137

98.773

0.475
  pilT Legionella pneumophila strain Lp02

48.137

98.773

0.475
  pilT Acinetobacter baumannii strain A118

47.095

100

0.472
  pilT Acinetobacter baumannii D1279779

47.095

100

0.472
  pilT Pseudomonas aeruginosa PAK

46.789

100

0.469
  pilT Acinetobacter nosocomialis M2

47.5

98.16

0.466
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

37.576

100

0.38
  pilU Vibrio cholerae strain A1552

38.509

98.773

0.38