Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1605_RS23420 Genome accession   NZ_CP143717
Coordinates   4811856..4812863 (-) Length   335 a.a.
NCBI ID   WP_217186909.1    Uniprot ID   -
Organism   Enterobacter soli strain ECC-115     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4806856..4817863
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1605_RS23385 (V1605_23310) - 4806978..4807475 (+) 498 WP_217186905.1 SprT family zinc-dependent metalloprotease -
  V1605_RS23390 (V1605_23315) endA 4807570..4808277 (+) 708 WP_217186906.1 deoxyribonuclease I -
  V1605_RS23395 (V1605_23320) rsmE 4808330..4809061 (+) 732 WP_217186907.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  V1605_RS23400 (V1605_23325) gshB 4809081..4810028 (+) 948 WP_182062370.1 glutathione synthase -
  V1605_RS23405 (V1605_23330) - 4810103..4810663 (+) 561 WP_014071695.1 YqgE/AlgH family protein -
  V1605_RS23410 (V1605_23335) ruvX 4810663..4811079 (+) 417 WP_014071696.1 Holliday junction resolvase RuvX -
  V1605_RS23415 (V1605_23340) - 4811073..4811795 (-) 723 WP_395265362.1 IclR family transcriptional regulator -
  V1605_RS23420 (V1605_23345) pilT 4811856..4812863 (-) 1008 WP_217186909.1 type IV pilus twitching motility protein PilT Machinery gene
  V1605_RS23425 (V1605_23350) - 4812881..4813585 (+) 705 WP_217186910.1 YggS family pyridoxal phosphate-dependent enzyme -
  V1605_RS23430 (V1605_23355) - 4813604..4814170 (+) 567 WP_182062375.1 YggT family protein -
  V1605_RS23435 (V1605_23360) yggU 4814167..4814463 (+) 297 WP_217186911.1 DUF167 family protein YggU -
  V1605_RS23440 (V1605_23365) - 4814467..4815060 (+) 594 WP_217186912.1 XTP/dITP diphosphatase -
  V1605_RS23445 (V1605_23370) hemW 4815053..4816195 (+) 1143 WP_217186913.1 radical SAM family heme chaperone HemW -
  V1605_RS23450 (V1605_23375) - 4816259..4816597 (+) 339 WP_217186914.1 endonuclease domain-containing protein -
  V1605_RS23455 (V1605_23380) - 4816680..4817399 (-) 720 WP_014071705.1 DUF2884 domain-containing protein -
  V1605_RS23460 (V1605_23385) - 4817453..4817779 (-) 327 WP_182062380.1 YggL family protein -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 36687.75 Da        Isoelectric Point: 6.7171

>NTDB_id=930899 V1605_RS23420 WP_217186909.1 4811856..4812863(-) (pilT) [Enterobacter soli strain ECC-115]
MVMEEMVALSVKHNVSDLHLCSDSPPRWRRAGTLEPAPFPPPDITQLLKTWLNDEQQGTWWANGQVDFAVTLASQQRLRG
SAFSHINGTSLTLRLLPASCPQLSALGAPRTLPVLLDNDSGLILVTGATGSGKSTTLAAMVDHLNRHYDKHILTLEDPVE
FIYQSDRCLIHQREIGQHCPSFAEALRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLCRDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSFAER
RAQGRLHERSVVASG

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=930899 V1605_RS23420 WP_217186909.1 4811856..4812863(-) (pilT) [Enterobacter soli strain ECC-115]
GTGGTGATGGAAGAAATGGTGGCCCTTAGTGTAAAGCATAACGTGTCGGATCTACACCTGTGCAGTGATTCACCGCCACG
CTGGCGCAGGGCTGGTACGCTTGAACCTGCGCCATTCCCGCCGCCGGATATTACGCAGCTATTAAAAACGTGGCTTAACG
ATGAGCAACAGGGGACCTGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGCTCGCCAGCCAGCAGCGTTTACGCGGC
AGCGCATTCAGCCACATTAACGGTACCTCACTCACGCTCAGGCTCTTGCCAGCAAGCTGCCCGCAGCTTTCGGCGCTGGG
GGCGCCGCGTACGCTCCCGGTACTGCTGGACAACGACAGCGGGCTGATTCTGGTGACGGGGGCAACCGGAAGTGGCAAAT
CCACTACGCTTGCGGCAATGGTGGATCATCTTAACCGCCACTACGATAAACACATTTTGACGCTGGAAGACCCGGTTGAG
TTTATTTATCAAAGCGATCGCTGTCTTATCCATCAGCGGGAGATAGGCCAGCACTGCCCTTCATTTGCAGAGGCGCTTCG
CGCCGCATTACGTGAAGATCCTGACGTGATTTTATTAGGGGAGCTTCGCGACAGCGAAACGATCAGGCTGGCCCTTACGG
CTGCGGAAACTGGGCATCTGGTGCTGGCAACGCTGCATACGCGTGGGGCAGCGCAGGCAATAGAGCGCCTGGTCGATACC
TTTCCGGCACAGGAAAAAGACCCGGTGCGTAATCAACTGGCGGGCAGTCTGCGCGCAGTGCTGGCGCAAAAGCTTTGTCG
GGACGTCCAGGGAGGACGCGTGGCGCTGTACGAGCTGCTGGTGAATACCTCAGCGGCAGCAAACCTTATCCGCGAGGGCA
AAACCTGGCAGTTGCCCGGTATTATCCAGACCGGGCAACAGGCAGGGATGCAGAATTTTGACCAAAGCTTTGCCGAACGC
AGAGCACAGGGGCGGCTTCACGAAAGGTCAGTCGTTGCGTCGGGTTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(66-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae strain A1552

50.305

97.91

0.493
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.305

97.91

0.493
  pilT Pseudomonas stutzeri DSM 10701

47.095

97.612

0.46
  pilT Neisseria meningitidis 8013

46.951

97.91

0.46
  pilT Neisseria gonorrhoeae MS11

46.646

97.91

0.457
  pilT Legionella pneumophila strain ERS1305867

47.205

96.119

0.454
  pilT Legionella pneumophila strain Lp02

47.205

96.119

0.454
  pilT Acinetobacter baumannii strain A118

46.483

97.612

0.454
  pilT Acinetobacter baylyi ADP1

46.483

97.612

0.454
  pilT Acinetobacter baumannii D1279779

46.483

97.612

0.454
  pilT Pseudomonas aeruginosa PAK

46.177

97.612

0.451
  pilT Acinetobacter nosocomialis M2

46.875

95.522

0.448
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.188

95.522

0.403
  pilU Pseudomonas stutzeri DSM 10701

38.485

98.507

0.379
  pilU Vibrio cholerae strain A1552

38.037

97.313

0.37