Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1580_RS21745 Genome accession   NZ_CP143687
Coordinates   4534826..4535806 (-) Length   326 a.a.
NCBI ID   WP_395277973.1    Uniprot ID   -
Organism   Enterobacter bugandensis strain ECC-190     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4529826..4540806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1580_RS21715 (V1580_21690) - 4530716..4531213 (+) 498 WP_236278301.1 SprT family zinc-dependent metalloprotease -
  V1580_RS21720 (V1580_21695) endA 4531308..4532015 (+) 708 WP_192987106.1 deoxyribonuclease I -
  V1580_RS21725 (V1580_21700) rsmE 4532067..4532798 (+) 732 WP_069732722.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  V1580_RS21730 (V1580_21705) gshB 4532818..4533765 (+) 948 WP_155026349.1 glutathione synthase -
  V1580_RS21735 (V1580_21710) - 4533840..4534400 (+) 561 WP_008499752.1 YqgE/AlgH family protein -
  V1580_RS21740 (V1580_21715) ruvX 4534400..4534816 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  V1580_RS21745 (V1580_21720) pilT 4534826..4535806 (-) 981 WP_395277973.1 type IV pilus twitching motility protein PilT Machinery gene
  V1580_RS21750 (V1580_21725) - 4535824..4536525 (+) 702 WP_395277974.1 YggS family pyridoxal phosphate-dependent enzyme -
  V1580_RS21755 (V1580_21730) - 4536547..4537113 (+) 567 WP_045260768.1 YggT family protein -
  V1580_RS21760 (V1580_21735) yggU 4537110..4537406 (+) 297 WP_047364545.1 DUF167 family protein YggU -
  V1580_RS21765 (V1580_21740) - 4537410..4538003 (+) 594 WP_010435473.1 XTP/dITP diphosphatase -
  V1580_RS21770 (V1580_21745) hemW 4537996..4539138 (+) 1143 WP_395277975.1 radical SAM family heme chaperone HemW -
  V1580_RS21775 (V1580_21750) - 4539202..4539540 (+) 339 WP_150145033.1 endonuclease domain-containing protein -
  V1580_RS21780 (V1580_21755) - 4539623..4540339 (-) 717 WP_041908321.1 DUF2884 domain-containing protein -
  V1580_RS21785 (V1580_21760) - 4540397..4540723 (-) 327 WP_028014375.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35496.51 Da        Isoelectric Point: 6.1627

>NTDB_id=930658 V1580_RS21745 WP_395277973.1 4534826..4535806(-) (pilT) [Enterobacter bugandensis strain ECC-190]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVAGDQRLRG
SAFKQMKGISVTLRLLPRSCPQLPSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNSHTDGHILTLEDPVE
FLYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=930658 V1580_RS21745 WP_395277973.1 4534826..4535806(-) (pilT) [Enterobacter bugandensis strain ECC-190]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTGGGCCGCCTTGAGCCGGCCCCGTTTCCGCCGCCGGATGTCGGGGCGTTGCTAAAGGCGTGGCTCAACG
ACGAACAGCAGGGTGCATGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTCGCAGGAGACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCATCTCCGTTACCCTGCGGCTGTTGCCGCGTTCCTGTCCGCAGCTCCCTTCACTTGG
GGTGCCGCGCGCTATTCCCGAACTGTTGTCCAGCGACAATGGGTTGATTCTGGTAACGGGAGCGACCGGCAGCGGTAAAT
CCACCACGCTGGCCGCAATGGTCGACTTCCTCAACAGCCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTTTGTACCAGAGTGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCAGAGGCGCTGCG
CAGTGCGTTACGCGAGGACCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGGGCAGCACAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCAGTGCTGGCGCAAAAGCTGCTTCC
CGATCTTCAGGGCGGGCGCGTCGCGCTGTACGAGCTGCTGGTAAACACCGCGGCGGCGGCGAATCTGATTCGTGAGGGGA
AGACGTGGCAGCTGCCCGGCATCATTCAAACCGGCCAGCAGGCGGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCACAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

50

98.773

0.494
  pilT Legionella pneumophila strain ERS1305867

50

98.773

0.494
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Pseudomonas aeruginosa PAK

48.012

100

0.482
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.547

98.773

0.42
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39