Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1603_RS22910 Genome accession   NZ_CP143680
Coordinates   4826438..4827418 (-) Length   326 a.a.
NCBI ID   WP_116341958.1    Uniprot ID   -
Organism   Enterobacter sp. ECC-219     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4821438..4832418
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1603_RS22880 (V1603_22845) - 4822327..4822824 (+) 498 WP_003860035.1 SprT family zinc-dependent metalloprotease -
  V1603_RS22885 (V1603_22850) endA 4822919..4823626 (+) 708 WP_166712308.1 deoxyribonuclease I -
  V1603_RS22890 (V1603_22855) rsmE 4823678..4824409 (+) 732 WP_014833222.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  V1603_RS22895 (V1603_22860) gshB 4824429..4825376 (+) 948 WP_023331278.1 glutathione synthase -
  V1603_RS22900 (V1603_22865) - 4825451..4826011 (+) 561 WP_020883695.1 YqgE/AlgH family protein -
  V1603_RS22905 (V1603_22870) ruvX 4826011..4826427 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  V1603_RS22910 (V1603_22875) pilT 4826438..4827418 (-) 981 WP_116341958.1 type IV pilus twitching motility protein PilT Machinery gene
  V1603_RS22915 (V1603_22880) - 4827436..4828137 (+) 702 WP_116341960.1 YggS family pyridoxal phosphate-dependent enzyme -
  V1603_RS22920 (V1603_22885) - 4828159..4828725 (+) 567 WP_116341962.1 YggT family protein -
  V1603_RS22925 (V1603_22890) yggU 4828722..4829018 (+) 297 WP_014885193.1 DUF167 family protein YggU -
  V1603_RS22930 (V1603_22895) - 4829022..4829615 (+) 594 WP_010435473.1 XTP/dITP diphosphatase -
  V1603_RS22935 (V1603_22900) hemW 4829608..4830750 (+) 1143 WP_395313307.1 radical SAM family heme chaperone HemW -
  V1603_RS22940 (V1603_22905) - 4830814..4831152 (+) 339 WP_166712314.1 endonuclease domain-containing protein -
  V1603_RS22945 (V1603_22910) - 4831235..4831951 (-) 717 WP_166712316.1 DUF2884 domain-containing protein -
  V1603_RS22950 (V1603_22915) - 4832008..4832334 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35719.76 Da        Isoelectric Point: 6.1647

>NTDB_id=930590 V1603_RS22910 WP_116341958.1 4826438..4827418(-) (pilT) [Enterobacter sp. ECC-219]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGTWWANGQVDFAVTVAEDQRLRG
SAFKQMKGISVTLRLLPRTCPQLSSLGVPRAIPELLSSDNGLVLVTGATGSGKSTTLAAMVDFLNRHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFVEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=930590 V1603_RS22910 WP_116341958.1 4826438..4827418(-) (pilT) [Enterobacter sp. ECC-219]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
TTGGCGCAGATTAGGCCGCCTTGAACCGGCGCCGTTTCCGCCGCCTGATGTAGGGGCGTTGTTGAAAGCCTGGCTCAACG
ACGAGCAGCAGGGAACCTGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTGGCGGAAGACCAGCGTCTGCGCGGG
AGCGCGTTTAAGCAGATGAAGGGGATATCCGTCACCCTGCGGCTGTTGCCGCGCACCTGTCCGCAGCTTTCCTCGCTGGG
TGTACCGCGGGCCATTCCCGAACTCTTGTCCAGCGACAACGGGTTGGTTCTGGTAACGGGAGCGACCGGCAGCGGCAAAT
CCACCACGCTGGCCGCAATGGTCGATTTTCTCAACCGCCATACTGACGGCCACATCCTCACGCTCGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCAGCACAGTCCATCATTTGTGGAGGCGCTGCG
CAGTGCATTACGCGAGGACCCGGACGTTATTTTACTGGGTGAGCTGCGCGACAGCGAAACAATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTCCACACTCGCGGGGCATCGCAGGCAATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCGGTGCTGGCGCAAAAGCTGCTTCC
CGATCTGCAGGGCGGGCGCGTCGCGTTGTATGAACTGCTGGTGAACACCGCAGCGGCGGCGAATCTTATTCGTGAAGGAA
AAACGTGGCAGTTGCCCGGCATTATTCAAACCGGCCAGCAGGCAGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCACAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Legionella pneumophila strain Lp02

50

98.773

0.494
  pilT Legionella pneumophila strain ERS1305867

50

98.773

0.494
  pilT Pseudomonas stutzeri DSM 10701

48.624

100

0.488
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Pseudomonas aeruginosa PAK

47.706

100

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.752

98.773

0.393