Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   V1587_RS20745 Genome accession   NZ_CP143660
Coordinates   4371091..4372071 (-) Length   326 a.a.
NCBI ID   WP_371897841.1    Uniprot ID   -
Organism   Enterobacter sp. ECC-249     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4366091..4377071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  V1587_RS20715 (V1587_20700) - 4366967..4367464 (+) 498 WP_371897842.1 SprT family zinc-dependent metalloprotease -
  V1587_RS20720 (V1587_20705) endA 4367559..4368266 (+) 708 WP_021242074.1 deoxyribonuclease I -
  V1587_RS20725 (V1587_20710) rsmE 4368318..4369049 (+) 732 WP_021242075.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  V1587_RS20730 (V1587_20715) gshB 4369069..4370016 (+) 948 WP_024906557.1 glutathione synthase -
  V1587_RS20735 (V1587_20720) - 4370104..4370664 (+) 561 WP_028014368.1 YqgE/AlgH family protein -
  V1587_RS20740 (V1587_20725) ruvX 4370664..4371080 (+) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  V1587_RS20745 (V1587_20730) pilT 4371091..4372071 (-) 981 WP_371897841.1 type IV pilus twitching motility protein PilT Machinery gene
  V1587_RS20750 (V1587_20735) - 4372089..4372790 (+) 702 WP_371897840.1 YggS family pyridoxal phosphate-dependent enzyme -
  V1587_RS20755 (V1587_20740) - 4372812..4373378 (+) 567 WP_336668952.1 YggT family protein -
  V1587_RS20760 (V1587_20745) yggU 4373375..4373671 (+) 297 WP_023309131.1 DUF167 family protein YggU -
  V1587_RS20765 (V1587_20750) - 4373675..4374268 (+) 594 WP_371897839.1 XTP/dITP diphosphatase -
  V1587_RS20770 (V1587_20755) hemW 4374261..4375403 (+) 1143 WP_371897838.1 radical SAM family heme chaperone HemW -
  V1587_RS20775 (V1587_20760) - 4375467..4375829 (+) 363 WP_371897837.1 endonuclease domain-containing protein -
  V1587_RS20780 (V1587_20765) - 4375891..4376607 (-) 717 WP_257260149.1 DUF2884 domain-containing protein -
  V1587_RS20785 (V1587_20770) - 4376664..4376990 (-) 327 WP_014885198.1 YggL family protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35498.51 Da        Isoelectric Point: 6.6964

>NTDB_id=930478 V1587_RS20745 WP_371897841.1 4371091..4372071(-) (pilT) [Enterobacter sp. ECC-249]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRTGRLEPAPFPPPDVGALLKTWLNDEQQGIWWASGQVDFAATVAGGQRLRG
SAFKQMRGISVTLRLLPRTCPQLASLGAPRAIPELLSNDAGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FIYQSERCLIQQREIGQHSPSFAEALRSALRQDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGASQAIERLVDT
FPAQEKDPVRNQLAGSMRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=930478 V1587_RS20745 WP_371897841.1 4371091..4372071(-) (pilT) [Enterobacter sp. ECC-249]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCCCG
CTGGCGAAGAACGGGCCGCCTTGAACCGGCACCGTTTCCTCCTCCTGACGTCGGGGCGTTGTTAAAAACGTGGCTCAACG
ATGAGCAGCAGGGGATATGGTGGGCCAGCGGGCAGGTGGATTTTGCCGCGACGGTGGCGGGAGGCCAGCGCCTGCGCGGC
AGTGCCTTTAAGCAGATGCGCGGTATCTCTGTGACGCTGCGGCTGTTGCCGCGTACCTGTCCGCAGCTCGCTTCACTGGG
CGCACCGCGGGCCATCCCGGAACTATTGTCCAATGACGCCGGGTTGATTCTGGTGACGGGGGCGACCGGCAGCGGTAAAT
CCACGACGCTGGCGGCGATGGTCGATTTTCTCAACCACCACACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATCTACCAGAGCGAGCGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCCGAAGCGCTGCG
CAGTGCCTTGCGCCAGGATCCTGACGTGATTCTGCTCGGGGAGTTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAAACCGGGCATCTGGTGCTGGCGACCCTGCATACGCGCGGGGCATCGCAGGCGATTGAGCGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCATGCGTGCGGTGCTGGCGCAGAAACTGCTTCC
CGATCTTCAGGGCGGGCGCGTCGCGCTGTATGAACTGCTGGTGAATACCGCCGCGGCGGCAAATCTCATTCGTGAAGGGA
AAACGTGGCAACTGCCCGGCATCATTCAAACCGGTCAGCAGGCGGGCATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCGCAGGGGCGGCTGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(74-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Legionella pneumophila strain ERS1305867

49.379

98.773

0.488
  pilT Legionella pneumophila strain Lp02

49.379

98.773

0.488
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Neisseria meningitidis 8013

47.866

100

0.482
  pilT Neisseria gonorrhoeae MS11

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.706

100

0.479
  pilT Acinetobacter baylyi ADP1

47.706

100

0.479
  pilT Acinetobacter baumannii D1279779

47.706

100

0.479
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Acinetobacter nosocomialis M2

48.125

98.16

0.472
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.478

98.773

0.429
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

38.82

98.773

0.383