Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VJ374_RS11510 Genome accession   NZ_CP142850
Coordinates   2170563..2171609 (-) Length   348 a.a.
NCBI ID   WP_329468826.1    Uniprot ID   -
Organism   Exiguobacterium sp. 9-2     
Function   pseudopilus biogenesis (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2165563..2176609
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VJ374_RS11490 (VJ374_11490) - 2166114..2167853 (-) 1740 WP_329468823.1 fimbrial assembly protein -
  VJ374_RS11495 (VJ374_11495) - 2167872..2168627 (-) 756 WP_290751826.1 A24 family peptidase -
  VJ374_RS11500 (VJ374_11500) - 2168696..2169202 (-) 507 WP_329468824.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VJ374_RS11505 (VJ374_11505) - 2169341..2170558 (-) 1218 WP_329468825.1 type II secretion system F family protein -
  VJ374_RS11510 (VJ374_11510) pilT 2170563..2171609 (-) 1047 WP_329468826.1 type IV pilus twitching motility protein PilT Machinery gene
  VJ374_RS11515 (VJ374_11515) - 2171606..2173270 (-) 1665 WP_329468827.1 ATPase, T2SS/T4P/T4SS family -
  VJ374_RS11520 (VJ374_11520) - 2173279..2173641 (-) 363 WP_329468828.1 hypothetical protein -
  VJ374_RS11525 (VJ374_11525) - 2173610..2174968 (-) 1359 WP_329468829.1 G5 domain-containing protein -
  VJ374_RS11530 (VJ374_11530) - 2175128..2175622 (+) 495 WP_311246621.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  VJ374_RS11535 (VJ374_11535) - 2175576..2176163 (+) 588 WP_329468830.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -

Sequence

Protein


Download         Length: 348 a.a.        Molecular weight: 38172.85 Da        Isoelectric Point: 6.8507

>NTDB_id=927367 VJ374_RS11510 WP_329468826.1 2170563..2171609(-) (pilT) [Exiguobacterium sp. 9-2]
MNSTIERAQIEDILRSSKEQDASDVHITAGSPPVFRINGTLAPFGTEKMKPIIIDGFVRSLITDEMYQQLKQDRDIDFSF
GVPGVARYRVNCFYQRGALSIAFRTIPTEVPTLEQLALPPVLKRFLTKPHGLILVTGPTGSGKSTTLAAMINEINQTMYK
RIITLEDPIEYLHSHHKSLIDQREIGIDAPRFATGLRSALRQDPDVILLGEMRDLETIATAVTAAETGHLVFATVHTSTA
ASTVSRIIDVFPSEQQDQIRAQLANVLAGVISQRLMPRSDGTGRVAALEIMVDTPAVSNLIRTGKEYQIQSVLQTGKQYG
MQTMQMALQDLVSRGLIPASAATPYISG

Nucleotide


Download         Length: 1047 bp        

>NTDB_id=927367 VJ374_RS11510 WP_329468826.1 2170563..2171609(-) (pilT) [Exiguobacterium sp. 9-2]
ATGAACAGTACGATTGAACGTGCCCAAATTGAGGACATACTACGTTCCTCTAAAGAACAAGATGCATCAGATGTCCACAT
CACGGCGGGATCACCACCCGTTTTTCGAATTAATGGAACGTTAGCGCCGTTTGGTACGGAGAAAATGAAGCCAATCATCA
TTGATGGATTCGTTCGATCCCTGATTACGGACGAAATGTATCAACAATTAAAACAAGATCGGGACATCGATTTTTCGTTT
GGAGTACCGGGCGTTGCTCGATATCGGGTCAATTGTTTTTATCAACGGGGCGCATTATCGATAGCATTTCGAACAATCCC
GACAGAAGTACCAACACTTGAGCAATTAGCATTACCTCCTGTCCTAAAGCGTTTTTTGACGAAACCGCACGGCTTGATTC
TTGTGACAGGTCCGACCGGTTCCGGAAAATCGACGACGCTTGCAGCGATGATCAATGAAATCAATCAGACAATGTATAAA
CGCATCATCACGCTCGAAGATCCAATCGAATACTTACATAGTCATCATAAAAGTCTGATTGATCAGCGGGAAATTGGAAT
CGATGCGCCACGCTTTGCTACGGGATTACGGTCGGCGCTCCGTCAGGATCCGGATGTTATCTTGCTCGGTGAGATGCGGG
ATCTTGAGACGATCGCAACTGCCGTTACGGCTGCTGAAACCGGTCACCTTGTCTTTGCGACGGTCCATACGTCAACCGCT
GCTTCGACGGTCAGTCGGATCATCGATGTCTTTCCGTCAGAACAACAAGATCAGATTCGAGCTCAGCTGGCGAACGTCCT
CGCTGGCGTCATCTCACAACGCTTGATGCCCCGCTCAGACGGAACAGGACGTGTCGCGGCACTCGAGATTATGGTCGATA
CACCAGCCGTCTCGAACTTGATTCGGACCGGTAAGGAATATCAGATTCAATCTGTCTTACAGACAGGTAAACAATATGGC
ATGCAGACGATGCAAATGGCGTTGCAGGACCTCGTCAGCCGAGGGTTGATTCCGGCATCTGCTGCAACACCGTACATATC
GGGGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(120-277)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

50.599

95.977

0.486
  pilT Neisseria gonorrhoeae MS11

50.599

95.977

0.486
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.61

94.253

0.477
  pilT Acinetobacter nosocomialis M2

49.552

96.264

0.477
  pilT Pseudomonas aeruginosa PAK

49.55

95.69

0.474
  pilT Acinetobacter baumannii D1279779

49.254

96.264

0.474
  pilT Acinetobacter baumannii strain A118

49.254

96.264

0.474
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.697

94.828

0.471
  pilT Vibrio cholerae strain A1552

49.697

94.828

0.471
  pilT Pseudomonas stutzeri DSM 10701

48.348

95.69

0.463
  pilT Acinetobacter baylyi ADP1

49.085

94.253

0.463
  pilT Legionella pneumophila strain Lp02

47.305

95.977

0.454
  pilT Legionella pneumophila strain ERS1305867

47.305

95.977

0.454
  pilU Acinetobacter baylyi ADP1

40

96.264

0.385
  pilU Pseudomonas stutzeri DSM 10701

39.514

94.54

0.374