Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VPX56_RS19005 Genome accession   NZ_CP142124
Coordinates   4000209..4001189 (-) Length   326 a.a.
NCBI ID   WP_326468291.1    Uniprot ID   -
Organism   Enterobacter wuhouensis strain AV1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3995209..4006189
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VPX56_RS18975 (VPX56_18975) - 3996084..3996581 (+) 498 WP_131633572.1 SprT family zinc-dependent metalloprotease -
  VPX56_RS18980 (VPX56_18980) endA 3996676..3997383 (+) 708 WP_131633571.1 deoxyribonuclease I -
  VPX56_RS18985 (VPX56_18985) rsmE 3997436..3998167 (+) 732 WP_326468286.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VPX56_RS18990 (VPX56_18990) gshB 3998187..3999134 (+) 948 WP_326468287.1 glutathione synthase -
  VPX56_RS18995 (VPX56_18995) - 3999222..3999782 (+) 561 WP_131633568.1 YqgE/AlgH family protein -
  VPX56_RS19000 (VPX56_19000) ruvX 3999782..4000198 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  VPX56_RS19005 (VPX56_19005) pilT 4000209..4001189 (-) 981 WP_326468291.1 type IV pilus twitching motility protein PilT Machinery gene
  VPX56_RS19010 (VPX56_19010) - 4001207..4001908 (+) 702 WP_131633566.1 YggS family pyridoxal phosphate-dependent enzyme -
  VPX56_RS19015 (VPX56_19015) - 4001930..4002496 (+) 567 WP_131633565.1 YggT family protein -
  VPX56_RS19020 (VPX56_19020) yggU 4002493..4002789 (+) 297 WP_028018385.1 DUF167 family protein YggU -
  VPX56_RS19025 (VPX56_19025) - 4002793..4003386 (+) 594 WP_326468295.1 XTP/dITP diphosphatase -
  VPX56_RS19030 (VPX56_19030) hemW 4003379..4004521 (+) 1143 WP_326468297.1 radical SAM family heme chaperone HemW -
  VPX56_RS19035 (VPX56_19035) - 4004585..4004947 (+) 363 WP_326468299.1 endonuclease domain-containing protein -
  VPX56_RS19040 (VPX56_19040) - 4005096..4005812 (-) 717 WP_131633561.1 DUF2884 domain-containing protein -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35658.68 Da        Isoelectric Point: 6.2330

>NTDB_id=921877 VPX56_RS19005 WP_326468291.1 4000209..4001189(-) (pilT) [Enterobacter wuhouensis strain AV1]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVEALLKRWLNDEQQGAWWANGQVDFAVTLAGNQRLRG
SAFNHMLGVSITLRLLPLACPQLSALGVPRAIPELLANDNGLILVTGATGSGKSTTLAAMVDFLNHHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRGALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
KAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=921877 VPX56_RS19005 WP_326468291.1 4000209..4001189(-) (pilT) [Enterobacter wuhouensis strain AV1]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCGCCGCG
CTGGCGCAGATTAGGTCGGCTTGAGCCGGCCCCCTTTCCGCCTCCTGACGTCGAAGCGCTATTAAAAAGATGGCTTAACG
ACGAACAGCAGGGCGCGTGGTGGGCAAACGGGCAGGTGGATTTTGCCGTCACGCTCGCAGGCAACCAGCGCCTGCGCGGC
AGCGCGTTTAACCATATGTTGGGCGTCTCGATAACGCTGCGGCTACTGCCGCTTGCTTGTCCGCAGCTTTCCGCACTGGG
CGTGCCCCGGGCGATCCCGGAGCTGCTAGCGAACGACAACGGGCTGATTCTGGTGACGGGGGCAACCGGCAGCGGTAAAT
CCACGACGCTCGCGGCAATGGTGGATTTTCTCAATCATCATACTGACGGCCATATCCTGACGCTCGAAGATCCGGTTGAA
TTTATGTACCAGAGCGAACGCTGTCTGATTCAGCAGCGCGAGATCGGACAGCACAGCCCCTCCTTTGCCGAGGCGCTGCG
CGGTGCGCTGCGTGAAGATCCGGATGTTATCCTGCTGGGTGAACTGCGCGATAGCGAAACCATCCGCCTGGCGCTGACCG
CGGCAGAAACCGGGCATCTGGTGCTGGCAACGCTGCATACGCGCGGCGCGGCGCAGGCCATAGAGCGGCTGGTGGATACC
TTCCCTGCGCAGGAGAAAGATCCGGTGCGTAATCAGCTGGCGGGCAGCCTGCGCGCCGTGCTGGCGCAAAAGCTGCGTCA
GGATGTTCAGGGCGGGCGCGTGGCGCTGTATGAACTTTTAGTGAATACGTCGGCGGCAGCAAACCTTATCCGTGAAGGGA
AAACCTGGCAGCTGCCTGGCATCATACAAACCGGCCAGCAGGCGGGAATGCAGAATTTCGATCAAAGCCTGGCGGAGAGA
AAGGCGCAGGGGCGGCTATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(109-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.153

100

0.503
  pilT Vibrio cholerae strain A1552

50.153

100

0.503
  pilT Pseudomonas stutzeri DSM 10701

48.318

100

0.485
  pilT Legionella pneumophila strain Lp02

48.758

98.773

0.482
  pilT Legionella pneumophila strain ERS1305867

48.758

98.773

0.482
  pilT Neisseria meningitidis 8013

47.561

100

0.479
  pilT Acinetobacter baumannii strain A118

47.401

100

0.475
  pilT Acinetobacter baylyi ADP1

47.401

100

0.475
  pilT Acinetobacter baumannii D1279779

47.401

100

0.475
  pilT Pseudomonas aeruginosa PAK

47.401

100

0.475
  pilT Neisseria gonorrhoeae MS11

47.256

100

0.475
  pilT Acinetobacter nosocomialis M2

47.812

98.16

0.469
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.034

99.08

0.426
  pilU Pseudomonas stutzeri DSM 10701

38.485

100

0.39
  pilU Vibrio cholerae strain A1552

38.199

98.773

0.377