Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VK758_RS18715 Genome accession   NZ_CP141897
Coordinates   3877444..3878424 (+) Length   326 a.a.
NCBI ID   WP_041908325.1    Uniprot ID   A0AAU7N8L0
Organism   Enterobacter bugandensis strain Eltt.P1     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3872444..3883424
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VK758_RS18680 (VK758_18680) - 3872807..3873133 (+) 327 WP_028014375.1 YggL family protein -
  VK758_RS18685 (VK758_18685) - 3873191..3873907 (+) 717 WP_041908321.1 DUF2884 domain-containing protein -
  VK758_RS18690 (VK758_18690) hemW 3874112..3875254 (-) 1143 WP_324660752.1 radical SAM family heme chaperone HemW -
  VK758_RS18695 (VK758_18695) - 3875247..3875840 (-) 594 WP_324660753.1 XTP/dITP diphosphatase -
  VK758_RS18700 (VK758_18700) yggU 3875844..3876140 (-) 297 WP_014885193.1 DUF167 family protein YggU -
  VK758_RS18705 (VK758_18705) - 3876137..3876703 (-) 567 WP_045260768.1 YggT family protein -
  VK758_RS18710 (VK758_18710) - 3876725..3877426 (-) 702 WP_047367421.1 YggS family pyridoxal phosphate-dependent enzyme -
  VK758_RS18715 (VK758_18715) pilT 3877444..3878424 (+) 981 WP_041908325.1 type IV pilus twitching motility protein PilT Machinery gene
  VK758_RS18720 (VK758_18720) ruvX 3878435..3878851 (-) 417 WP_021242076.1 Holliday junction resolvase RuvX -
  VK758_RS18725 (VK758_18725) - 3878851..3879411 (-) 561 WP_028014368.1 YqgE/AlgH family protein -
  VK758_RS18730 (VK758_18730) gshB 3879486..3880433 (-) 948 WP_155026349.1 glutathione synthase -
  VK758_RS18735 (VK758_18735) rsmE 3880453..3881184 (-) 732 WP_041908327.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VK758_RS18740 (VK758_18740) endA 3881236..3881943 (-) 708 WP_028014365.1 deoxyribonuclease I -
  VK758_RS18745 (VK758_18745) - 3882038..3882535 (-) 498 WP_028014364.1 SprT family zinc-dependent metalloprotease -

Sequence

Protein


Download         Length: 326 a.a.        Molecular weight: 35583.65 Da        Isoelectric Point: 6.3904

>NTDB_id=920417 VK758_RS18715 WP_041908325.1 3877444..3878424(+) (pilT) [Enterobacter bugandensis strain Eltt.P1]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRLGRLEPAPFPPPDVGALLKAWLNDEQQGAWWANGQVDFAVTVAGDQRLRG
SAFKQMKGISVTLRLLPRSCPQLPSLGVPRAIPELLSSDNGLILVTGATGSGKSTTLAAMVDFLNRHTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALRSALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDT
FPAQEKDPVRNQLAGSLRAVLAQKLLPDLQGGRVALYELLVNTAAAANLIREGKTWQLPGIIQTGQQAGMQNFDQSLAER
RAQGRL

Nucleotide


Download         Length: 981 bp        

>NTDB_id=920417 VK758_RS18715 WP_041908325.1 3877444..3878424(+) (pilT) [Enterobacter bugandensis strain Eltt.P1]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCACCACCGCG
CTGGCGCAGATTGGGCCGCCTTGAGCCGGCCCCGTTTCCGCCGCCGGATGTCGGGGCGTTGCTAAAGGCGTGGCTCAACG
ACGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTCGATTTTGCCGTGACGGTCGCAGGAGACCAGCGTCTGCGCGGC
AGTGCGTTTAAGCAGATGAAGGGCATCTCCGTTACCTTGCGGCTGTTGCCGCGTTCCTGTCCGCAGCTCCCTTCGCTTGG
TGTGCCGCGCGCTATTCCCGAACTGTTGTCCAGCGACAATGGGTTGATTCTGGTAACGGGAGCGACCGGCAGCGGTAAAT
CTACCACGCTGGCCGCAATGGTCGACTTCCTCAACCGCCATACTGACGGCCATATCCTCACGCTTGAAGATCCGGTGGAG
TTTATGTACCAGAGTGAACGTTGCCTGATTCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCATTTGCAGAGGCGCTGCG
CAGTGCGTTACGCGAGGACCCGGACGTTATTTTGCTGGGGGAGCTGCGCGACAGCGAAACGATCCGCCTGGCGCTGACGG
CGGCGGAGACCGGGCATCTGGTGCTGGCGACGCTGCACACGCGCGGGGCAGCACAGGCGATTGAACGGCTGGTCGATACG
TTCCCGGCGCAGGAGAAAGATCCGGTGCGTAACCAGCTGGCCGGCAGCCTGCGGGCAGTGCTGGCGCAAAAGCTGCTTCC
CGATCTTCAGGGCGGGCGCGTCGCGCTGTACGAGCTGCTGGTAAACACCGCGGCGGCGGCGAATCTGATTCGTGAGGGGA
AGACGTGGCAGCTGCCCGGCATCATTCAAACCGGCCAGCAGGCGGGAATGCAGAACTTTGACCAGAGCCTGGCGGAGAGA
CGGGCACAGGGGCGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(90-266)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

50.459

100

0.506
  pilT Vibrio cholerae strain A1552

50.459

100

0.506
  pilT Pseudomonas stutzeri DSM 10701

48.93

100

0.491
  pilT Legionella pneumophila strain Lp02

49.689

98.773

0.491
  pilT Legionella pneumophila strain ERS1305867

49.689

98.773

0.491
  pilT Neisseria meningitidis 8013

48.171

100

0.485
  pilT Acinetobacter baumannii strain A118

48.318

100

0.485
  pilT Acinetobacter baylyi ADP1

48.318

100

0.485
  pilT Acinetobacter baumannii D1279779

48.318

100

0.485
  pilT Neisseria gonorrhoeae MS11

47.866

100

0.482
  pilT Pseudomonas aeruginosa PAK

48.012

100

0.482
  pilT Acinetobacter nosocomialis M2

48.75

98.16

0.479
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.857

98.773

0.423
  pilU Pseudomonas stutzeri DSM 10701

39.091

100

0.396
  pilU Vibrio cholerae strain A1552

39.441

98.773

0.39