Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   VI606_RS17235 Genome accession   NZ_CP141537
Coordinates   3553431..3554399 (-) Length   322 a.a.
NCBI ID   WP_324641981.1    Uniprot ID   -
Organism   Enterobacter hormaechei strain EB3_SW1_43.22_ST134     
Function   type IV pilus retraction (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3548431..3559399
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VI606_RS17205 (VI606_17205) - 3549309..3549806 (+) 498 WP_015571833.1 SprT family zinc-dependent metalloprotease -
  VI606_RS17210 (VI606_17210) endA 3549901..3550608 (+) 708 WP_045347336.1 deoxyribonuclease I -
  VI606_RS17215 (VI606_17215) rsmE 3550660..3551391 (+) 732 WP_017692692.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  VI606_RS17220 (VI606_17220) gshB 3551423..3552370 (+) 948 WP_015571835.1 glutathione synthase -
  VI606_RS17225 (VI606_17225) - 3552445..3553005 (+) 561 WP_006811924.1 YqgE/AlgH family protein -
  VI606_RS17230 (VI606_17230) ruvX 3553005..3553421 (+) 417 WP_006811925.1 Holliday junction resolvase RuvX -
  VI606_RS17235 (VI606_17235) pilT 3553431..3554399 (-) 969 WP_324641981.1 type IV pilus twitching motility protein PilT Machinery gene
  VI606_RS17240 (VI606_17240) - 3554417..3555118 (+) 702 WP_045347340.1 YggS family pyridoxal phosphate-dependent enzyme -
  VI606_RS17245 (VI606_17245) - 3555140..3555706 (+) 567 WP_015571838.1 YggT family protein -
  VI606_RS17250 (VI606_17250) yggU 3555703..3555999 (+) 297 WP_023304404.1 DUF167 family protein YggU -
  VI606_RS17255 (VI606_17255) - 3556003..3556596 (+) 594 WP_045327517.1 XTP/dITP diphosphatase -
  VI606_RS17260 (VI606_17260) hemW 3556589..3557737 (+) 1149 WP_017382926.1 radical SAM family heme chaperone HemW -
  VI606_RS17265 (VI606_17265) - 3557794..3558132 (+) 339 WP_045347341.1 endonuclease domain-containing protein -
  VI606_RS17270 (VI606_17270) - 3558214..3558930 (-) 717 WP_015571842.1 DUF2884 domain-containing protein -
  VI606_RS17275 (VI606_17275) - 3558987..3559313 (-) 327 WP_003862421.1 YggL family protein -

Sequence

Protein


Download         Length: 322 a.a.        Molecular weight: 35279.18 Da        Isoelectric Point: 6.2335

>NTDB_id=917177 VI606_RS17235 WP_324641981.1 3553431..3554399(-) (pilT) [Enterobacter hormaechei strain EB3_SW1_43.22_ST134]
MDVEEIVALSVKHNVSDLHLCSDSPPRWRRSGRLEPAPFPPPDVEALLKAWLNDEQQGAWWANGQVDFAVTLAGRQRLRG
SAFKHMHGVSIALRLLPLTCPQLSALGVPRAIPELLSNDNGLILVTGATGSGKSTTLAAMVDFLNHQTDGHILTLEDPVE
FMYQSERCLIQQREIGQHSPSFAEALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAIERLVDTFPAQ
EKDPVRNQLAGSLRAVLAQKLRQDVQGGRVALYELLVNTSAAANLIREGKTWQLPGIIQTGQQAGMQNFEQSLAERRAQG
RL

Nucleotide


Download         Length: 969 bp        

>NTDB_id=917177 VI606_RS17235 WP_324641981.1 3553431..3554399(-) (pilT) [Enterobacter hormaechei strain EB3_SW1_43.22_ST134]
ATGGATGTGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCCGATCTACACCTGTGCAGTGATTCGCCACCGCG
CTGGCGCAGGTCAGGCCGTCTTGAACCTGCGCCGTTTCCGCCCCCGGATGTGGAAGCATTATTAAAAGCGTGGCTCAACG
ATGAACAGCAGGGCGCATGGTGGGCAAACGGGCAGGTGGATTTTGCCGTTACCCTCGCAGGCCGTCAGCGGCTGCGCGGC
AGTGCGTTTAAGCATATGCACGGCGTTTCGATTGCGCTGCGGCTGTTGCCGCTGACGTGCCCGCAGCTCTCTGCGTTAGG
TGTGCCGCGAGCGATCCCGGAGCTGCTGTCCAATGACAATGGCCTGATTCTGGTCACCGGCGCCACCGGCAGCGGAAAAT
CGACCACCCTGGCCGCGATGGTCGATTTCCTCAATCATCAGACAGACGGGCATATTCTGACCCTGGAAGATCCGGTGGAG
TTTATGTACCAGAGCGAACGTTGCCTGATCCAGCAGCGGGAGATAGGCCAGCACAGCCCGTCCTTTGCCGAGGCGCTGCG
TGAAGATCCGGATGTGATTCTGCTTGGTGAGCTGCGCGACAGCGAGACGATACGCCTGGCGCTGACGGCGGCGGAAACCG
GCCATCTGGTGTTAGCCACGCTGCATACGCGCGGTGCGGCGCAGGCGATCGAGCGCCTGGTCGACACCTTTCCGGCTCAG
GAGAAAGATCCGGTGCGTAATCAGCTGGCGGGAAGCCTGCGTGCGGTCCTGGCGCAGAAGCTACGTCAGGATGTCCAGGG
CGGGCGCGTGGCGCTGTATGAGCTACTGGTGAATACCTCGGCGGCGGCAAACCTGATCCGCGAAGGCAAAACCTGGCAAC
TGCCGGGCATTATTCAAACGGGGCAGCAGGCGGGAATGCAGAACTTTGAGCAGAGTCTGGCGGAGCGACGGGCGCAGGGG
CGGCTGTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(89-261)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

49.235

100

0.5
  pilT Vibrio cholerae strain A1552

49.235

100

0.5
  pilT Legionella pneumophila strain ERS1305867

47.516

100

0.475
  pilT Legionella pneumophila strain Lp02

47.516

100

0.475
  pilT Pseudomonas stutzeri DSM 10701

46.789

100

0.475
  pilT Neisseria meningitidis 8013

46.341

100

0.472
  pilT Neisseria gonorrhoeae MS11

46.341

100

0.472
  pilT Pseudomonas aeruginosa PAK

45.872

100

0.466
  pilT Acinetobacter baumannii D1279779

45.872

100

0.466
  pilT Acinetobacter baumannii strain A118

45.872

100

0.466
  pilT Acinetobacter baylyi ADP1

45.566

100

0.463
  pilT Acinetobacter nosocomialis M2

46.25

99.379

0.46
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

42.415

100

0.425
  pilU Pseudomonas stutzeri DSM 10701

37.879

100

0.388
  pilU Vibrio cholerae strain A1552

37.267

100

0.373